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- PDB-7k0m: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa/OR... -

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Basic information

Entry
Database: PDB / ID: 7k0m
TitleHuman serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa/ORMDL3, class 1
Components
  • (Serine palmitoyltransferase ...Serine C-palmitoyltransferase) x 3
  • ORM1-like protein 3
KeywordsMEMBRANE PROTEIN / Sphingolipid
Function / homology
Function and homology information


negative regulation of serine C-palmitoyltransferase activity / negative regulation of ceramide biosynthetic process / cellular sphingolipid homeostasis / sphinganine biosynthetic process / regulation of fat cell apoptotic process / serine C-palmitoyltransferase complex / sphingomyelin biosynthetic process / SPOTS complex / serine C-palmitoyltransferase / serine C-palmitoyltransferase activity ...negative regulation of serine C-palmitoyltransferase activity / negative regulation of ceramide biosynthetic process / cellular sphingolipid homeostasis / sphinganine biosynthetic process / regulation of fat cell apoptotic process / serine C-palmitoyltransferase complex / sphingomyelin biosynthetic process / SPOTS complex / serine C-palmitoyltransferase / serine C-palmitoyltransferase activity / regulation of smooth muscle contraction / sphingosine biosynthetic process / sphingolipid biosynthetic process / ceramide biosynthetic process / ceramide metabolic process / positive regulation of lipophagy / sphingolipid metabolic process / motor behavior / negative regulation of B cell apoptotic process / myelination / adipose tissue development / specific granule membrane / positive regulation of autophagy / secretory granule membrane / protein localization / positive regulation of protein localization to nucleus / pyridoxal phosphate binding / endoplasmic reticulum membrane / neutrophil degranulation / endoplasmic reticulum / integral component of membrane / plasma membrane / cytoplasm
ORM1-like protein 3 / Small subunit of serine palmitoyltransferase-like / Pyridoxal phosphate-dependent transferase / Pyridoxal phosphate-dependent transferase domain 1 / Pyridoxal phosphate-dependent transferase, major domain / ORMDL family / Aminotransferase, class I/classII / Aminotransferase, class-II, pyridoxal-phosphate binding site
Serine palmitoyltransferase 1 / Serine palmitoyltransferase 2 / ORM1-like protein 3 / Serine palmitoyltransferase small subunit A
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsWang, Y. / Niu, Y. / Zhang, Z. / Zhao, H. / Myasnikov, A. / Kalathur, R. / Lee, C.H.
CitationJournal: Nat Struct Mol Biol / Year: 2021
Title: Structural insights into the regulation of human serine palmitoyltransferase complexes.
Authors: Yingdi Wang / Yiming Niu / Zhe Zhang / Kenneth Gable / Sita D Gupta / Niranjanakumari Somashekarappa / Gongshe Han / Hongtu Zhao / Alexander G Myasnikov / Ravi C Kalathur / Teresa M Dunn / Chia-Hsueh Lee /
Abstract: Sphingolipids are essential lipids in eukaryotic membranes. In humans, the first and rate-limiting step of sphingolipid synthesis is catalyzed by the serine palmitoyltransferase holocomplex, which ...Sphingolipids are essential lipids in eukaryotic membranes. In humans, the first and rate-limiting step of sphingolipid synthesis is catalyzed by the serine palmitoyltransferase holocomplex, which consists of catalytic components (SPTLC1 and SPTLC2) and regulatory components (ssSPTa and ORMDL3). However, the assembly, substrate processing and regulation of the complex are unclear. Here, we present 8 cryo-electron microscopy structures of the human serine palmitoyltransferase holocomplex in various functional states at resolutions of 2.6-3.4 Å. The structures reveal not only how catalytic components recognize the substrate, but also how regulatory components modulate the substrate-binding tunnel to control enzyme activity: ssSPTa engages SPTLC2 and shapes the tunnel to determine substrate specificity. ORMDL3 blocks the tunnel and competes with substrate binding through its amino terminus. These findings provide mechanistic insights into sphingolipid biogenesis governed by the serine palmitoyltransferase complex.
Validation Report
SummaryFull reportAbout validation report
History
DepositionSep 4, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 24, 2021Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2021Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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Structure visualization

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Assembly

Deposited unit
A: Serine palmitoyltransferase 1
B: Serine palmitoyltransferase 2
C: Serine palmitoyltransferase small subunit A
D: ORM1-like protein 3
G: Serine palmitoyltransferase small subunit A
H: ORM1-like protein 3
E: Serine palmitoyltransferase 1
F: Serine palmitoyltransferase 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)290,42024
Polymers279,2858
Non-polymers11,13516
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551
Buried area39840 Å2
ΔGint-263 kcal/mol
Surface area91180 Å2

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Components

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Serine palmitoyltransferase ... , 3 types, 6 molecules AEBFCG

#1: Protein Serine palmitoyltransferase 1 / Serine C-palmitoyltransferase / Long chain base biosynthesis protein 1 / LCB 1 / Serine-palmitoyl-CoA transferase 1 / SPT1


Mass: 52806.770 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SPTLC1, LCB1 / Production host: Homo sapiens (human) / References: UniProt: O15269, serine C-palmitoyltransferase
#2: Protein Serine palmitoyltransferase 2 / Serine C-palmitoyltransferase / Long chain base biosynthesis protein 2 / LCB 2 / Long chain base biosynthesis protein 2a / LCB2a / ...Long chain base biosynthesis protein 2 / LCB 2 / Long chain base biosynthesis protein 2a / LCB2a / Serine-palmitoyl-CoA transferase 2 / SPT 2


Mass: 60851.902 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SPTLC2, KIAA0526, LCB2 / Production host: Homo sapiens (human) / References: UniProt: O15270, serine C-palmitoyltransferase
#3: Protein Serine palmitoyltransferase small subunit A / Serine C-palmitoyltransferase / Small subunit of serine palmitoyltransferase A / ssSPTa


Mass: 8471.095 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SPTSSA, C14orf147, SSSPTA / Production host: Homo sapiens (human) / References: UniProt: Q969W0

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Protein , 1 types, 2 molecules DH

#4: Protein ORM1-like protein 3


Mass: 17512.594 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ORMDL3 / Production host: Homo sapiens (human) / References: UniProt: Q8N138

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Non-polymers , 2 types, 16 molecules

#5: Chemical ChemComp-PLP / PYRIDOXAL-5'-PHOSPHATE / VITAMIN B6 Phosphate / Pyridoxal phosphate


Mass: 247.142 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H10NO6P / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical
ChemComp-POV / (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / POPC / POPC


Mass: 760.076 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: C42H82NO8P / Comment: phospholipid*YM

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: human serine palmitoyltransferase complexes / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
MicroscopyModel: FEI TALOS ARCTICA
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 72.53 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.18.2_3874: / Classification: refinement
CTF correctionType: NONE
3D reconstructionResolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 169428 / Symmetry type: POINT
Refine LS restraints
Refinement-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00318964
ELECTRON MICROSCOPYf_angle_d0.52425620
ELECTRON MICROSCOPYf_dihedral_angle_d21.147024
ELECTRON MICROSCOPYf_chiral_restr0.0432854
ELECTRON MICROSCOPYf_plane_restr0.0043242

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