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- PDB-7joy: Product structure of SARS-CoV-2 Mpro C145A mutant in complex with... -

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Basic information

Entry
Database: PDB / ID: 7joy
TitleProduct structure of SARS-CoV-2 Mpro C145A mutant in complex with its C-terminal autoprocessing sequence.
Components3C-like proteinase
KeywordsVIRAL PROTEIN / Protease / SARS-CoV-2
Function / homology
Function and homology information


protein guanylyltransferase activity / RNA endonuclease activity, producing 3'-phosphomonoesters / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Maturation of replicase proteins / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs ...protein guanylyltransferase activity / RNA endonuclease activity, producing 3'-phosphomonoesters / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Maturation of replicase proteins / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs / Translation of Replicase and Assembly of the Replication Transcription Complex / snRNP Assembly / Replication of the SARS-CoV-2 genome / TRAF3-dependent IRF activation pathway / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / : / double membrane vesicle viral factory outer membrane / SARS coronavirus main proteinase / host cell endosome / 3'-5'-RNA exonuclease activity / host cell endoplasmic reticulum-Golgi intermediate compartment / symbiont-mediated suppression of host toll-like receptor signaling pathway / symbiont-mediated degradation of host mRNA / mRNA guanylyltransferase / symbiont-mediated suppression of host ISG15-protein conjugation / G-quadruplex RNA binding / SARS-CoV-2 modulates host translation machinery / omega peptidase activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / host cell Golgi apparatus / symbiont-mediated perturbation of host ubiquitin-like protein modification / mRNA (nucleoside-2'-O-)-methyltransferase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / ubiquitinyl hydrolase 1 / DNA helicase / cysteine-type deubiquitinase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / single-stranded RNA binding / host cell endoplasmic reticulum membrane / host cell perinuclear region of cytoplasm / viral protein processing / lyase activity / RNA helicase / induction by virus of host autophagy / RNA-directed RNA polymerase / copper ion binding / symbiont-mediated suppression of host gene expression / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / lipid binding / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding / membrane / identical protein binding
Similarity search - Function
RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / : / : / Coronavirus Nonstructural protein 13, 1B domain / Coronavirus Non-structural protein 13, zinc-binding domain / Coronavirus Nonstructural protein 13, stalk domain / : / Coronavirus Nsp12 Interface domain profile. ...RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / : / : / Coronavirus Nonstructural protein 13, 1B domain / Coronavirus Non-structural protein 13, zinc-binding domain / Coronavirus Nonstructural protein 13, stalk domain / : / Coronavirus Nsp12 Interface domain profile. / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / NSP12 RNA-dependent RNA polymerase, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / Nidovirus 2-O-methyltransferase / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Nidovirus 3'-5' exoribonuclease domain / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Nonstructural protein 13, 1B domain, coronavirus / Arterivirus Nsp11 N-terminal/Coronavirus NSP15 middle domain / Non-structural protein NSP15, N-terminal domain superfamily, coronavirus / Non-structural protein NSP15, middle domain superfamily / Coronavirus replicase NSP15, N-terminal oligomerization / Nonstructural protein 15, middle domain, coronavirus / Coronavirus replicase NSP15, middle domain / Coronavirus replicase NSP15, N-terminal oligomerisation / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. / Non-structural protein NSP16, coronavirus-like / Non-structural protein 14, coronavirus / RNA polymerase, N-terminal, coronavirus / Coronavirus 2'-O-methyltransferase / Coronavirus proofreading exoribonuclease / Coronavirus RNA-dependent RNA polymerase, N-terminal / Nidoviral uridylate-specific endoribonuclease (NendoU) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain / Nonstructural protein 13, zinc-binding domain, coronavirus-like / Coronaviridae zinc-binding (CV ZBD) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Endoribonuclease EndoU-like / NendoU domain, nidovirus / Coronavirus replicase NSP15, uridylate-specific endoribonuclease / DNA2/NAM7 helicase-like, C-terminal / AAA domain / (+) RNA virus helicase core domain / (+)RNA virus helicase core domain profile. / Lipocalin signature. / Non-structural protein NSP3, SUD-N (Mac2) domain, betacoronavirus / Sarbecovirus Nsp3c-N domain profile. / Non-structural protein NSP3, N-terminal, betacoronavirus / Polyprotein cleavage domain PL2pro superfamily, betacoronavirus / Non-structural protein NSP3, SUD-N (Mac2) domain superfamily, betacoronavirus / Betacoronavirus SUD-C domain / Betacoronavirus replicase NSP3, N-terminal / NSP1 globular domain superfamily, betacoronavirus / Non-structural protein 2, SARS-CoV-like / Carbamoyl-phosphate synthase subdomain signature 2. / Coronavirus 3Ecto domain profile. / : / Betacoronavirus Nsp3e group 2-specific marker (G2M) domain profile. / NSP1, C-terminal domain, betacoronavirus / Betacoronavirus Nsp3c-M domain profile. / NSP1, globular domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain superfamily, betacoronavirus / Betacoronavirus replicase NSP1 / Betacoronavirus single-stranded poly(A) binding domain / Betacoronavirus (BetaCoV) Nsp1 C-terminal domain profile. / Betacoronavirus Nsp3c-C domain profile. / Betacoronavirus Nsp3e nucleic acid-binding (NAB) domain profile. / DPUP/SUD, C-terminal, betacoronavirus / Non-structural protein NSP3, nucleic acid-binding domain superfamily, betacoronavirus / Non-structural protein 6, betacoronavirus / Betacoronavirus nucleic acid-binding (NAB) / Non-structural protein NSP3, nucleic acid-binding domain, betacoronavirus / Non-structural protein NSP3A domain-like superfamily / Papain-like protease, N-terminal domain superfamily, coronavirus / Papain-like viral protease, palm and finger domains, coronavirus / : / Coronavirus (CoV) Nsp2 middle domain profile. / Coronavirus (CoV) Nsp2 N-terminal domain profile. / Coronavirus (CoV) Nsp2 C-terminal domain profile. / NSP1, globular domain, alpha/betacoronavirus / : / Coronavirus (CoV) Nsp3 Y domain profile. / Coronavirus (CoV) Nsp1 globular domain profile. / Coronavirus replicase NSP2, N-terminal / Nonstructural protein 2, N-terminal domain, coronavirus / Coronavirus replicase NSP2, C-terminal / Non-structural protein 2, C-terminal domain, coronavirus / Coronavirus Nsp3a Ubl domain profile. / Coronavirus Nsp3d Ubl domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp7 cofactor domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp8 cofactor domain profile. / Coronavirus Nsp9 single-stranded RNA (ssRNA)-binding domain profile. / Coronavirus (CoV) ExoN/MTase coactivator domain profile. / NSP3, first ubiquitin-like (Ubl) domain, coronavirus / NSP3, second ubiquitin-like (Ubl) domain, coronavirus
Similarity search - Domain/homology
Replicase polyprotein 1ab
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsLee, J. / Worrall, L.J. / Paetzel, M. / Strynadka, N.C.J.
Funding support Canada, 1items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR) Canada
CitationJournal: Nat Commun / Year: 2020
Title: Crystallographic structure of wild-type SARS-CoV-2 main protease acyl-enzyme intermediate with physiological C-terminal autoprocessing site.
Authors: Lee, J. / Worrall, L.J. / Vuckovic, M. / Rosell, F.I. / Gentile, F. / Ton, A.T. / Caveney, N.A. / Ban, F. / Cherkasov, A. / Paetzel, M. / Strynadka, N.C.J.
History
DepositionAug 7, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 7, 2020Provider: repository / Type: Initial release
Revision 2.0Nov 4, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Refinement description
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / pdbx_nonpoly_scheme / pdbx_refine_tls / pdbx_refine_tls_group / pdbx_struct_assembly_prop / refine / reflns / reflns_shell / software
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site_anisotrop.U[1][2] / _atom_site_anisotrop.U[2][3] / _pdbx_nonpoly_scheme.auth_seq_num / _pdbx_struct_assembly_prop.value / _refine.ls_R_factor_R_free / _refine.ls_R_factor_R_work / _refine.ls_R_factor_obs / _reflns.pdbx_CC_half / _reflns.pdbx_Rpim_I_all / _reflns.pdbx_Rrim_I_all / _reflns.pdbx_number_measured_all / _reflns.pdbx_scaling_rejects / _software.classification / _software.name / _software.version
Description: Model orientation/position
Details: Model position within unit cell updated with the ACHESYM server. No other changes made.
Provider: author / Type: Coordinate replacement
Revision 2.1Dec 2, 2020Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 2.2Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 3C-like proteinase
B: 3C-like proteinase


Theoretical massNumber of molelcules
Total (without water)67,5872
Polymers67,5872
Non-polymers00
Water4,828268
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: light scattering, Dimer in AU
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2850 Å2
ΔGint-12 kcal/mol
Surface area26140 Å2
MethodPISA
Unit cell
Length a, b, c (Å)123.665, 80.313, 63.319
Angle α, β, γ (deg.)90.000, 90.200, 90.000
Int Tables number5
Space group name H-MC121

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Components

#1: Protein 3C-like proteinase / 3C-like proteinas


Mass: 33793.480 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Production host: Escherichia coli (E. coli)
References: UniProt: P0DTD1, SARS coronavirus main proteinase
#2: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 268 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.33 Å3/Da / Density % sol: 47.12 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 / Details: 0.1 M MES pH 6, 16-22% PEG 3350, 5% MPD

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.5418 Å
DetectorType: DECTRIS PILATUS3 R 200K-A / Detector: PIXEL / Date: Jun 8, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 2→28.63 Å / Num. obs: 40349 / % possible obs: 96.3 % / Redundancy: 2.8 % / Biso Wilson estimate: 24.95 Å2 / Rmerge(I) obs: 0.138 / Net I/σ(I): 3.9
Reflection shellResolution: 2→2.05 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.872 / Num. unique obs: 2904 / % possible all: 93.6

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Processing

Software
NameVersionClassification
BUSTER2.10.3 (20-MAY-2020)refinement
Aimless0.7.4data scaling
PDB_EXTRACT3.25data extraction
xia2data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6LU7
Resolution: 2→28.63 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.91 / SU R Cruickshank DPI: 0.217 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.218 / SU Rfree Blow DPI: 0.178 / SU Rfree Cruickshank DPI: 0.179
RfactorNum. reflection% reflectionSelection details
Rfree0.252 1990 4.94 %RANDOM
Rwork0.217 ---
obs0.219 40282 96.1 %-
Displacement parametersBiso max: 83.91 Å2 / Biso mean: 34.37 Å2 / Biso min: 11.02 Å2
Baniso -1Baniso -2Baniso -3
1-1.4202 Å20 Å2-0.7217 Å2
2---2.5027 Å20 Å2
3---1.0825 Å2
Refine analyzeLuzzati coordinate error obs: 0.31 Å
Refinement stepCycle: final / Resolution: 2→28.63 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4729 0 0 268 4997
Biso mean---36.51 -
Num. residues----612
Refine LS restraints
Refine-IDTypeNumberRestraint functionWeightDev ideal
X-RAY DIFFRACTIONt_dihedral_angle_d1623SINUSOIDAL2
X-RAY DIFFRACTIONt_trig_c_planes
X-RAY DIFFRACTIONt_gen_planes830HARMONIC5
X-RAY DIFFRACTIONt_it4835HARMONIC10
X-RAY DIFFRACTIONt_nbd
X-RAY DIFFRACTIONt_improper_torsion
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_chiral_improper_torsion630SEMIHARMONIC5
X-RAY DIFFRACTIONt_sum_occupancies
X-RAY DIFFRACTIONt_utility_distance
X-RAY DIFFRACTIONt_utility_angle
X-RAY DIFFRACTIONt_utility_torsion
X-RAY DIFFRACTIONt_ideal_dist_contact4005SEMIHARMONIC4
X-RAY DIFFRACTIONt_bond_d4835HARMONIC20.008
X-RAY DIFFRACTIONt_angle_deg6573HARMONIC21
X-RAY DIFFRACTIONt_omega_torsion3.5
X-RAY DIFFRACTIONt_other_torsion18.6
LS refinement shellResolution: 2→2.02 Å / Rfactor Rfree error: 0 / Total num. of bins used: 51
RfactorNum. reflection% reflection
Rfree0.2306 35 4.34 %
Rwork0.2275 771 -
all0.2276 806 -
obs--90.12 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
11.2188-0.66210.72182.4882-1.48581.5357-0.01340.07430.0796-0.0320.0639-0.0918-0.06450.0357-0.05050.0620.0313-0.0382-0.00310.0011-0.121124.898551.79888.601
24.0499-1.30530.264.7761-0.42743.4913-0.08610.20290.3955-0.27210.19930.1074-0.7188-0.1867-0.11310.12930.0829-0.0293-0.09910.1004-0.199719.6262.04311.0415
30.6495-0.70360.55091.9236-0.69892.13970.0102-0.0234-0.0784-0.02650.05430.04490.0144-0.0263-0.06460.04030.0171-0.02530.00120.0126-0.078820.565244.002511.5469
40.8275-0.44150.43472.8651-1.14581.68710.02750.007-0.1036-0.0450.22660.5852-0.0125-0.3726-0.2541-0.02480.0214-0.02850.05670.047-0.041112.654241.564512.8914
53.9163-2.34232.13774.4498-2.20065.77280.40250.0978-0.7723-0.43740.28220.91720.8292-0.2305-0.68470.023-0.0728-0.204-0.18440.05120.027812.803718.347617.1269
64.49291.10960.08932.4011-1.00981.00830.2475-0.0751-0.0779-0.05130.04540.27780.1710.0307-0.29290.0225-0.0248-0.0898-0.06280.0305-0.073619.665424.716620.7477
71.74430.14050.39020.8964-0.32271.06980.00340.0490.25830.1430.2433-0.3486-0.15850.2363-0.2467-0.0190.0376-0.09770.0309-0.14-0.06844.146639.03817.8719
82.4932-0.4691.29495.9669-0.78847.13190.1320.3457-0.1880.18290.361-1.08790.20020.6087-0.493-0.29430.0454-0.1929-0.0061-0.25030.127458.537431.78518.3231
91.30810.3591-0.1832.1117-0.49552.61270.02790.01460.04590.39170.1474-0.2096-0.04580.0832-0.17540.06690.0341-0.1025-0.0221-0.0607-0.103841.070439.134524.697
103.4044-2.8578-3.90134.42483.43392.1625-0.2165-0.4471-0.13440.6580.28240.19230.35460.2054-0.06590.19390.0788-0.1416-0.06750.014-0.222334.11335.237836.904
110.37891.9045-0.75645.56463.06336.8169-0.1259-0.25530.23830.7457-0.3160.7430.4949-0.6680.4420.0136-0.04990.1296-0.0645-0.0246-0.095718.475342.198948.5129
124.5371-0.9926-0.57137.9484-0.22974.8139-0.17760.0398-0.0850.1890.04110.75140.1232-0.15620.13660.0406-0.00420.011-0.12830.0049-0.173221.281543.190142.4357
13-0.00471.23010.6845.41873.31663.0399-0.13630.09040.0709-0.9880.24790.221-0.1241-0.1669-0.11150.3281-0.1384-0.0984-0.0750.0464-0.218122.00855.654432.5817
141.21880.66210.72182.48821.48581.5357-0.0134-0.07430.07960.0320.06390.0918-0.0645-0.0357-0.05050.062-0.0313-0.0382-0.0031-0.0011-0.121136.823512.14423.0583
154.04991.30530.264.77610.42743.4913-0.0861-0.20290.39550.27210.1993-0.1074-0.71880.1867-0.11310.1293-0.0829-0.0293-0.0991-0.1004-0.199742.10222.388330.6178
160.64950.70360.55091.92360.69892.13970.01020.0234-0.07840.02650.0543-0.04490.01440.0263-0.06460.0403-0.0171-0.02530.0012-0.0126-0.078841.15684.347720.1124
170.82750.44150.43472.86511.14581.68710.0275-0.007-0.10360.0450.2266-0.5852-0.01250.3726-0.2541-0.0248-0.0214-0.02850.0567-0.047-0.041149.06781.909718.7679
183.91632.34232.13774.44982.20065.77280.4025-0.0978-0.77230.43740.2822-0.91720.82920.2305-0.68470.0230.0728-0.204-0.1844-0.05120.027848.9183-21.307214.5324
194.4929-1.10960.08932.40111.00981.00830.24750.0751-0.07790.05130.0454-0.27780.171-0.0307-0.29290.02250.0248-0.0898-0.0628-0.0305-0.073642.0566-14.938210.9116
201.7443-0.14050.39020.89640.32271.06980.0034-0.0490.2583-0.1430.24330.3486-0.1585-0.2363-0.2467-0.019-0.0376-0.09770.03090.14-0.06817.5754-0.616813.7874
212.49320.4691.29495.96690.78847.13190.132-0.3457-0.188-0.18290.3611.08790.2002-0.6087-0.493-0.2943-0.0454-0.1929-0.00610.25030.12743.1846-7.869813.3362
221.3081-0.3591-0.1832.11170.49552.61270.0279-0.01460.0459-0.39170.14740.2096-0.0458-0.0832-0.17540.0669-0.0341-0.1025-0.02210.0607-0.103820.6516-0.52036.9623
233.40442.8578-3.90134.4248-3.43392.1625-0.21650.4471-0.1344-0.6580.2824-0.19230.3546-0.2054-0.06590.1939-0.0788-0.1416-0.0675-0.014-0.222327.609-4.417-5.2447
240.3789-1.9045-0.75645.5646-3.06336.8169-0.12590.25530.2383-0.7457-0.316-0.7430.49490.6680.4420.01360.04990.1296-0.06450.0246-0.095743.24672.5441-16.8536
254.53710.9926-0.57137.94840.22974.8139-0.1776-0.0398-0.085-0.1890.0411-0.75140.12320.15620.13660.04060.00420.011-0.1283-0.0049-0.173240.44053.5353-10.7764
26-0.0047-1.23010.6845.4187-3.31663.0399-0.1363-0.09040.07090.9880.2479-0.221-0.12410.1669-0.11150.32810.1384-0.0984-0.075-0.0464-0.218139.71415.9996-0.9224
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth seq-ID
1X-RAY DIFFRACTION1{ A|1 - A|43 }0
2X-RAY DIFFRACTION2{ A|44 - A|100 }0
3X-RAY DIFFRACTION3{ A|101 - A|155 }0
4X-RAY DIFFRACTION4{ A|156 - A|213 }0
5X-RAY DIFFRACTION5{ A|214 - A|260 }0
6X-RAY DIFFRACTION6{ A|261 - A|306 }0
7X-RAY DIFFRACTION7{ B|1 - B|43 }0
8X-RAY DIFFRACTION8{ B|44 - B|91 }0
9X-RAY DIFFRACTION9{ B|92 - B|180 }0
10X-RAY DIFFRACTION10{ B|181 - B|213 }0
11X-RAY DIFFRACTION11{ B|214 - B|237 }0
12X-RAY DIFFRACTION12{ B|238 - B|292 }0
13X-RAY DIFFRACTION13{ B|293 - B|306 }0
14X-RAY DIFFRACTION1{ A|1 - A|43 }0
15X-RAY DIFFRACTION2{ A|44 - A|100 }0
16X-RAY DIFFRACTION3{ A|101 - A|155 }0
17X-RAY DIFFRACTION4{ A|156 - A|213 }0
18X-RAY DIFFRACTION5{ A|214 - A|260 }0
19X-RAY DIFFRACTION6{ A|261 - A|306 }0
20X-RAY DIFFRACTION7{ B|1 - B|43 }0
21X-RAY DIFFRACTION8{ B|44 - B|91 }0
22X-RAY DIFFRACTION9{ B|92 - B|180 }0
23X-RAY DIFFRACTION10{ B|181 - B|213 }0
24X-RAY DIFFRACTION11{ B|214 - B|237 }0
25X-RAY DIFFRACTION12{ B|238 - B|292 }0
26X-RAY DIFFRACTION13{ B|293 - B|306 }0

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