Mass: 17558.746 Da / Num. of mol.: 1 / Fragment: SUBSTRATE BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Strain: JM109(DE3) / Production host: Escherichia coli (E. coli) / Strain (production host): JM109(DE3) / References: UniProt: P63018
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
HNCA
1
2
1
HN(CA)HA
1
3
1
HN(CO)CA
1
4
1
HA(CACO)NH
1
5
1
CP H(C)CCH-TOCSY
1
6
1
CP (H)CCH-TOCSY
1
7
1
CP(H)C(CCACO)NH-TOCSY
1
8
1
15N-RESOLVED NOESY-HSQC
1
9
1
13CRESOLVED NOESY-HMQC
1
10
1
4D13C
1
11
1
13C RESOLVED HMQC-NOESY3D 13C
1
12
1
13N RESOLVED HMQC-NOESY-HSQC3D 13C
1
13
1
13C RESOLVED HMQC-NOESY-HSQC
1
14
1
-HSQC
NMR details
Text: ENERGY MINIMIZED AVERAGE. ASSIGNMENTS OBTAINED WITH TRIPLE RESONANCE NMR SPECTROSCOPY; STEREO-SPECIFIC ASSIGNMENTS WITH 10% 13C LABELED GLUCOSE; STRUCTURE WITH 2 HIGH RESOLUTION 4-D AND 6 3D ...Text: ENERGY MINIMIZED AVERAGE. ASSIGNMENTS OBTAINED WITH TRIPLE RESONANCE NMR SPECTROSCOPY; STEREO-SPECIFIC ASSIGNMENTS WITH 10% 13C LABELED GLUCOSE; STRUCTURE WITH 2 HIGH RESOLUTION 4-D AND 6 3D HETERONUCLEAR-RESOLVED NOESY EXPERIMENTS. SCALAR COUPLINGS WITH HNHA; DYNAMICS WITH T1 AND T2 (NITROGEN)
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Sample preparation
Details
Contents: 1.5 MM 15N OR 15N,13C LABELED PROTEIN 5%D20/95%H2O AND 100%D2O
Sample conditions
Ionic strength: 50 mM SODIUM PHOSPHATE / pH: 7.0 / Pressure: 1 atm / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker AMX 500
Bruker
AMX500
500
1
Bruker AMX 600
Bruker
AMX600
600
2
Bruker DMX 750
Bruker
DMX750
750
3
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Processing
NMR software
Name
Version
Developer
Classification
Discover
BIOSYM
refinement
Felix
structuresolution
MSI INSIGHT
INSIGHT
structuresolution
MSI DISCOVER
DISCOVER
structuresolution
Refinement
Method: DISTANCE GEOMETRY, SIMULATE ANNEALING / Software ordinal: 1 Details: STRUCTURE WAS EXTENSIVELY MINIMIZED. THE PROTOCOL CAN BE FOUND IN THE JMB PAPER (IN PRESS, 1999)
NMR ensemble
Conformer selection criteria: AVERAGE OF LOWEST TOTAL ENERGY Conformers calculated total number: 50 / Conformers submitted total number: 1
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