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- PDB-7fis: The crystal structure of beta-1,2-mannobiose phosphorylase in com... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7fis | ||||||
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Title | The crystal structure of beta-1,2-mannobiose phosphorylase in complex with mannose 1-phosphate (M1P) | ||||||
![]() | Beta-1,2-mannobiose phosphorylase | ||||||
![]() | TRANSFERASE / Mannobiose / thermoanaerobacter / mannose 1-phosphate | ||||||
Function / homology | beta-1,2-mannobiose phosphorylase / Mannoside phosphorylase / beta-1,4-mannooligosaccharide phosphorylase / GDP-mannose biosynthetic process / Glycosyl hydrolase, five-bladed beta-propellor domain superfamily / glycosyltransferase activity / 1-O-phosphono-alpha-D-mannopyranose / Beta-1,2-mannobiose phosphorylase![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Dai, L. / Chang, Z. / Yang, J. / Liu, W. / Yang, Y. / Chen, C.-C. / Zhang, L. / Huang, J. / Sun, Y. / Guo, R.-T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural investigation of a thermostable 1,2-beta-mannobiose phosphorylase from Thermoanaerobacter sp. X-514. Authors: Dai, L. / Chang, Z. / Yang, J. / Liu, W. / Yang, Y. / Chen, C.C. / Zhang, L. / Huang, J.W. / Sun, Y. / Guo, R.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 290.3 KB | Display | ![]() |
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PDB format | ![]() | 230.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.3 MB | Display | ![]() |
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Full document | ![]() | 2.4 MB | Display | |
Data in XML | ![]() | 58.3 KB | Display | |
Data in CIF | ![]() | 86.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7fipC ![]() 7fiqC ![]() 7firC ![]() 5b0rS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Sugars , 2 types, 9 molecules CBAD![](data/chem/img/M1P.gif)
![](data/chem/img/M1P.gif)
#1: Protein | Mass: 35856.902 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: X514 / Gene: Teth514_1789 / Production host: ![]() ![]() References: UniProt: B0K2C3, beta-1,2-mannobiose phosphorylase #2: Sugar | ChemComp-M1P / |
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-Non-polymers , 5 types, 1212 molecules ![](data/chem/img/PG4.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/1PE.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/1PE.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | ChemComp-ZN / #5: Chemical | ChemComp-1PE / | #6: Chemical | ChemComp-GOL / | #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.87 Å3/Da / Density % sol: 57.19 % |
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Crystal grow | Temperature: 298 K / Method: evaporation / Details: 10 mM ZnCl2, 24% PEG3350, 180 mM Tris-HCl pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: LIQUID ANODE / Type: BRUKER METALJET / Wavelength: 1.34138 Å |
Detector | Type: BRUKER PHOTON 100 / Detector: CMOS / Date: Nov 5, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.34138 Å / Relative weight: 1 |
Reflection | Resolution: 2.16→35.15 Å / Num. obs: 163537 / % possible obs: 99.8 % / Redundancy: 6 % / CC1/2: 0.994 / Rmerge(I) obs: 0.14 / Net I/σ(I): 6 |
Reflection shell | Resolution: 2.19→2.22 Å / Rmerge(I) obs: 0.498 / Mean I/σ(I) obs: 4.2 / Num. unique obs: 3249 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5B0R Resolution: 2.19→35.15 Å / SU ML: 0.25 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 23.21 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 92.46 Å2 / Biso mean: 27.3606 Å2 / Biso min: 5.21 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.19→35.15 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 28
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