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- PDB-7fbi: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5 -

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Basic information

Entry
Database: PDB / ID: 7fbi
TitleCryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
Components
  • 3A3 heavy chain
  • 3A3 light chain
  • 3A5 heavy chain
  • 3A5 light chain
  • Envelope glycoprotein B
KeywordsVIRAL PROTEIN / EBV / Glycoprotein B / Neutralizing antibody / Cryo-EM
Function / homology
Function and homology information


host cell Golgi membrane / host cell endosome membrane / membrane => GO:0016020 / symbiont entry into host cell / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane
Similarity search - Function
Herpesvirus Glycoprotein B / Herpesvirus Glycoprotein B, PH-like domain 1 / Herpesvirus Glycoprotein B, PH-like domain 2 / Herpesvirus Glycoprotein B / Herpesvirus Glycoprotein B PH-like domain / Herpesvirus Glycoprotein B, PH-like domain 2 superfamily
Similarity search - Domain/homology
Envelope glycoprotein B
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit)
Epstein-Barr virus (Epstein-Barr virus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsZheng, Q. / Li, S. / Zha, Z. / Hong, J. / Chen, Y. / Zhang, X.
CitationJournal: To Be Published
Title: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
Authors: Zheng, Q. / Li, S. / Zha, Z. / Hong, J. / Chen, Y. / Zhang, X.
History
DepositionJul 10, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 13, 2022Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: 3A3 heavy chain
L: 3A3 light chain
A: Envelope glycoprotein B
M: 3A5 light chain
N: 3A5 heavy chain


Theoretical massNumber of molelcules
Total (without water)120,9605
Polymers120,9605
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area6010 Å2
ΔGint-34 kcal/mol
Surface area63160 Å2

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Components

#1: Protein 3A3 heavy chain


Mass: 11744.192 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Oryctolagus cuniculus (rabbit) / Cell line (production host): 293F / Production host: Homo sapiens (human)
#2: Antibody 3A3 light chain


Mass: 11572.933 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Oryctolagus cuniculus (rabbit) / Cell line (production host): 293F / Production host: Homo sapiens (human)
#3: Protein Envelope glycoprotein B / gB


Mass: 73456.188 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Epstein-Barr virus (strain GD1) (Epstein-Barr virus)
Strain: GD1 / Gene: BALF4, gB, EBVaGC1_061 / Cell line (production host): 293F / Production host: Homo sapiens (human) / References: UniProt: R4R670
#4: Antibody 3A5 light chain


Mass: 11672.961 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Oryctolagus cuniculus (rabbit) / Cell line (production host): 293F / Production host: Homo sapiens (human)
#5: Protein 3A5 heavy chain


Mass: 12513.935 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Oryctolagus cuniculus (rabbit) / Cell line (production host): 293F / Production host: Homo sapiens (human)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 60 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.14_3259: / Classification: refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 367966 / Symmetry type: POINT

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