[English] 日本語
Yorodumi
- EMDB-31516: Cryo-EM structure of EBV gB in complex with nAb 3A3 -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-31516
TitleCryo-EM structure of EBV gB in complex with nAb 3A3
Map data
Sample
  • Complex: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
    • Protein or peptide: EBV gB
Biological speciesOryctolagus cuniculus (rabbit) / Human gammaherpesvirus 4 (Epstein-Barr virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.1 Å
AuthorsZheng Q / Li S / Zha Z / Chen Y / Hong J / Zhang X
CitationJournal: To Be Published
Title: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
Authors: Zheng Q / Li S / Zha Z / Hong J / Chen Y / Zhang X
History
DepositionJul 10, 2021-
Header (metadata) releaseJul 13, 2022-
Map releaseJul 13, 2022-
UpdateJul 13, 2022-
Current statusJul 13, 2022Processing site: PDBj / Status: Released

-
Structure visualization

Supplemental images

emd_31516.png

Downloads & links

-
Map

FileDownload / File: emd_31516.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.12 Å/pix.
x 256 pix.
= 286.72 Å		1.12 Å/pix.
x 256 pix.
= 286.72 Å		1.12 Å/pix.
x 256 pix.
= 286.72 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.12 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 1.5
Minimum - Maximum-1.4915543 - 3.6847138
Average (Standard dev.)-0.010602131 (±0.3138647)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 286.72 Å
α=β=γ: 90.0 °

-
Supplemental data

-
Sample components

-
Entire : Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5

EntireName: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
Components
  • Complex: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
    • Protein or peptide: EBV gB

-
Supramolecule #1: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5

SupramoleculeName: Cryo-EM structure of EBV gB in complex with nAbs 3A3 and 3A5
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Recombinant expressionOrganism: Homo sapiens (human)

-
Macromolecule #1: EBV gB

MacromoleculeName: EBV gB / type: protein_or_peptide / ID: 1 / Enantiomer: DEXTRO
Source (natural)Organism: Human gammaherpesvirus 4 (Epstein-Barr virus)
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AQTPEQPAPP ATTVQPTATR QQTSFPFRVC ELSSHGDLFR FSSDIQCPSF GTRENHTEGL LMVFKDNIIP YSFKVRSYTK IVTNILIYNG HRADSVTNRH EEKFSVESYE TDQMDTIYQC YNAVKMTKDG LTRVYVDRDG VNITVNLKPT GGLANGVRRY ASQTELYDAP ...String:
AQTPEQPAPP ATTVQPTATR QQTSFPFRVC ELSSHGDLFR FSSDIQCPSF GTRENHTEGL LMVFKDNIIP YSFKVRSYTK IVTNILIYNG HRADSVTNRH EEKFSVESYE TDQMDTIYQC YNAVKMTKDG LTRVYVDRDG VNITVNLKPT GGLANGVRRY ASQTELYDAP GRVEATYRTR TTVNCLITDM MAKSNSPFDF FVTTTGQTVE MSPFYDGKNT ETFHERADSF HVRTNYKIVD YDNRGTNPQG ERRAFLDKGT YTLSWKLENR TAYCPLQHWQ TFDSTIATET GKSIHFVTDE GTSSFVTNTT VGIELPDAFK CIEEQVNKTM HEKYEAVQDR YTKGQEAITY FITSGGLLLA WLPLTPRSLA TVKNLTELTT PTSSPPSSPS PPAPPAARGS TSAAVLRRRR RNAGNATTPV PPAAPGKSLG TLNNPATVQI QFAYDSLRRQ INRMLGDLAR AWCLEQKRQN MVLRELTKIN PTTVMSSIYG KAVAAKRLGD VISVSQCVPV NQATVTLRKS MRVPGSETMC YSRPLVSFSF INDTKTYEGQ LGTDNEIFLT KKMTEVCQAT SQYYFQSGNE IHVYNDYHHF KTIELDGIAT LQTFISLNTS LIENIDFASL ELYSRDEQRA SNVFDLEGIF REYNFQAQNI A

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TECNAI F30
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Tecnai F30 / Image courtesy: FEI Company

-
Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 7.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 9270
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more