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- PDB-7f5j: The crystal structure of VyPAL2-I244V, a more efficient mutant of... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7f5j | |||||||||
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Title | The crystal structure of VyPAL2-I244V, a more efficient mutant of VyPAL2 peptide asparaginyl ligase in its active enzyme form | |||||||||
![]() | Peptide Asparaginyl Ligases | |||||||||
![]() | PLANT PROTEIN / AEP / Legumain / Peptide ligase / PAL | |||||||||
Function / homology | ![]() vacuolar protein processing / vacuole / proteolysis involved in protein catabolic process / cysteine-type endopeptidase activity Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Hu, S. / Sahili, A. / Lescar, J. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for proenzyme maturation, substrate recognition, and ligation by a hyperactive peptide asparaginyl ligase. Authors: Hu, S. / El Sahili, A. / Kishore, S. / Wong, Y.H. / Hemu, X. / Goh, B.C. / Zhipei, S. / Wang, Z. / Tam, J.P. / Liu, C.F. / Lescar, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 242.4 KB | Display | ![]() |
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PDB format | ![]() | 193.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7f5pC ![]() 7f5qC ![]() 7fa0C ![]() 6idvS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 31118.818 Da / Num. of mol.: 2 / Mutation: I244V Source method: isolated from a genetically manipulated source Details: D171 (forming SNN), residue 172 is HD0 (HIS + SNN) / Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 3 types, 6 molecules ![](data/chem/img/NAG.gif)
#2: Polysaccharide | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#3: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#4: Sugar | ChemComp-NAG / |
-Non-polymers , 3 types, 510 molecules ![](data/chem/img/EDO.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-EDO / #6: Chemical | ChemComp-GOL / #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.65 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / Details: 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 20, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 1.593→46.06 Å / Num. obs: 74533 / % possible obs: 99.26 % / Redundancy: 13.4 % / Biso Wilson estimate: 29.35 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.08307 / Rpim(I) all: 0.02365 / Rrim(I) all: 0.08643 / Net I/σ(I): 16.5 |
Reflection shell | Resolution: 1.593→1.65 Å / Redundancy: 12.5 % / Mean I/σ(I) obs: 0.83 / Num. unique obs: 6869 / CC1/2: 0.449 / CC star: 0.787 / Rpim(I) all: 0.8825 / % possible all: 92.83 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6IDV Resolution: 1.593→46.06 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.95 / SU R Cruickshank DPI: 0.081 / Cross valid method: FREE R-VALUE / SU R Blow DPI: 0.086 / SU Rfree Blow DPI: 0.085 / SU Rfree Cruickshank DPI: 0.082
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Displacement parameters | Biso mean: 37.91 Å2
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Refine analyze | Luzzati coordinate error obs: 0.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.593→46.06 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.593→1.61 Å
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Refinement TLS params. | Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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