+Open data
-Basic information
Entry | Database: PDB / ID: 7el1 | |||||||||
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Title | Structure of a protein from bacteria | |||||||||
Components |
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Keywords | UNKNOWN FUNCTION | |||||||||
Function / homology | Function and homology information maintenance of CRISPR repeat elements / endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / DNA binding / RNA binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Staphylococcus aureus (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.22 Å | |||||||||
Authors | Liu, H. / Zhu, Y. / Huang, Z. | |||||||||
Funding support | China, 2items
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Citation | Journal: Nucleic Acids Res. / Year: 2021 Title: Structural basis of Staphylococcus aureus Cas9 inhibition by AcrIIA14. Authors: Liu, H. / Zhu, Y. / Lu, Z. / Huang, Z. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7el1.cif.gz | 300.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7el1.ent.gz | 233.6 KB | Display | PDB format |
PDBx/mmJSON format | 7el1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/el/7el1 ftp://data.pdbj.org/pub/pdb/validation_reports/el/7el1 | HTTPS FTP |
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-Related structure data
Related structure data | 5czzS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 2 molecules AE
#1: Protein | Mass: 124083.648 Da / Num. of mol.: 1 / Mutation: N580A, C946A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: cas9 / Production host: Escherichia coli (E. coli) References: UniProt: J7RUA5, Hydrolases; Acting on ester bonds |
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#5: Protein | Mass: 11937.598 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Production host: Escherichia coli (E. coli) |
-DNA chain , 2 types, 2 molecules CD
#3: DNA chain | Mass: 8469.482 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Staphylococcus aureus (bacteria) |
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#4: DNA chain | Mass: 2465.653 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Staphylococcus aureus (bacteria) |
-RNA chain / Non-polymers , 2 types, 127 molecules B
#2: RNA chain | Mass: 23525.975 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Staphylococcus aureus (bacteria) |
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#6: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.49 Å3/Da / Density % sol: 64.79 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 1.0M sodium citrate dihydrate, 0.3M imidazol-Hcl (pH 10.0), 0.02M sodium malonate (pH 7.0) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-BM / Wavelength: 0.9791 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jan 18, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.22→68.47 Å / Num. obs: 116344 / % possible obs: 99.9 % / Redundancy: 6.4 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 17 |
Reflection shell | Resolution: 2.22→2.34 Å / Rmerge(I) obs: 0.714 / Num. unique obs: 16935 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5czz Resolution: 2.22→50 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 2.22→50 Å
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LS refinement shell | Resolution: 2.22→2.34 Å /
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