+Open data
-Basic information
Entry | Database: PDB / ID: 7eec | ||||||
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Title | Crystal structure of EphA7 mutant G656R | ||||||
Components | Ephrin type-A receptor 7 | ||||||
Keywords | MEMBRANE PROTEIN / Kinase domain / Mutant | ||||||
Function / homology | Function and homology information regulation of protein autophosphorylation / regulation of cysteine-type endopeptidase activity involved in apoptotic process / negative regulation of synapse assembly / nephric duct morphogenesis / negative regulation of collateral sprouting / branching morphogenesis of a nerve / axon guidance receptor activity / regulation of cell-cell adhesion / GPI-linked ephrin receptor activity / chemorepellent activity ...regulation of protein autophosphorylation / regulation of cysteine-type endopeptidase activity involved in apoptotic process / negative regulation of synapse assembly / nephric duct morphogenesis / negative regulation of collateral sprouting / branching morphogenesis of a nerve / axon guidance receptor activity / regulation of cell-cell adhesion / GPI-linked ephrin receptor activity / chemorepellent activity / transmembrane-ephrin receptor activity / EPH-Ephrin signaling / regulation of postsynapse organization / negative chemotaxis / retinal ganglion cell axon guidance / EPHA-mediated growth cone collapse / growth factor binding / EPH-ephrin mediated repulsion of cells / ephrin receptor signaling pathway / regulation of peptidyl-tyrosine phosphorylation / regulation of ERK1 and ERK2 cascade / axon guidance / modulation of chemical synaptic transmission / brain development / receptor protein-tyrosine kinase / positive regulation of neuron apoptotic process / protein tyrosine kinase activity / neuron apoptotic process / positive regulation of protein phosphorylation / phosphorylation / glutamatergic synapse / dendrite / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | Chakraborty, S. / Varma, A.K. | ||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2021 Title: Crystal structure of clinically reported mutations Gly656Arg, Gly656Glu and Asp751His identified in the kinase domain of EphA7. Authors: Chakraborty, S. / Varma, A.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7eec.cif.gz | 69.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7eec.ent.gz | 46.7 KB | Display | PDB format |
PDBx/mmJSON format | 7eec.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7eec_validation.pdf.gz | 430.4 KB | Display | wwPDB validaton report |
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Full document | 7eec_full_validation.pdf.gz | 434.1 KB | Display | |
Data in XML | 7eec_validation.xml.gz | 11.7 KB | Display | |
Data in CIF | 7eec_validation.cif.gz | 15 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ee/7eec ftp://data.pdbj.org/pub/pdb/validation_reports/ee/7eec | HTTPS FTP |
-Related structure data
Related structure data | 7eedC 7eefC 2reiS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34682.926 Da / Num. of mol.: 1 / Mutation: G656R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: EPHA7, EHK3, HEK11 / Production host: Escherichia coli (E. coli) References: UniProt: Q15375, receptor protein-tyrosine kinase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 49.23 % |
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Crystal grow | Temperature: 295.15 K / Method: vapor diffusion, hanging drop / Details: PEG3350, ammonium sulphate, HEPES |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ROTATING ANODE / Type: BRUKER AXS MICROSTAR / Wavelength: 1.54184 Å |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Nov 11, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54184 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→72.03 Å / Num. obs: 5944 / % possible obs: 100 % / Redundancy: 1 % / CC1/2: 0.984 / Rmerge(I) obs: 0.175 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 3.1→3.27 Å / Rmerge(I) obs: 0.175 / Num. unique obs: 874 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2REI Resolution: 3.1→44.8428 Å / Cor.coef. Fo:Fc: 0.909 / Cor.coef. Fo:Fc free: 0.893 / SU B: 16.647 / SU ML: 0.294 / Cross valid method: FREE R-VALUE / ESU R Free: 0.415 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.018 Å2
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Refinement step | Cycle: LAST / Resolution: 3.1→44.8428 Å
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Refine LS restraints |
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LS refinement shell |
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