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- PDB-7e1s: Crystal structure of dehydrogenase/isomerase FabX from Helicobact... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7e1s | ||||||
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Title | Crystal structure of dehydrogenase/isomerase FabX from Helicobacter pylori in complex with octanoyl-ACP | ||||||
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![]() | FLAVOPROTEIN / Unsaturated fatty acid biosynthesis / FMN / 4Fe-4S cluster | ||||||
Function / homology | ![]() Kdo2-lipid A biosynthetic process / nitronate monooxygenase activity / dioxygenase activity / phosphopantetheine binding / acyl carrier activity / 4 iron, 4 sulfur cluster binding / nucleotide binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhou, J.S. / Zhang, L. / Zhang, L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis. Authors: Zhou, J. / Zhang, L. / Zeng, L. / Yu, L. / Duan, Y. / Shen, S. / Hu, J. / Zhang, P. / Song, W. / Ruan, X. / Jiang, J. / Zhang, Y. / Zhou, L. / Jia, J. / Hang, X. / Tian, C. / Lin, H. / Chen, ...Authors: Zhou, J. / Zhang, L. / Zeng, L. / Yu, L. / Duan, Y. / Shen, S. / Hu, J. / Zhang, P. / Song, W. / Ruan, X. / Jiang, J. / Zhang, Y. / Zhou, L. / Jia, J. / Hang, X. / Tian, C. / Lin, H. / Chen, H.Z. / Cronan, J.E. / Bi, H. / Zhang, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 182.3 KB | Display | ![]() |
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PDB format | ![]() | 141.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 36.9 KB | Display | |
Data in CIF | ![]() | 49.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7e1qC ![]() 7e1rC ![]() 4iqlS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 2 types, 4 molecules ACBD
#1: Protein | Mass: 40871.305 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: AM497_02530, B0X24_07955, B0X43_08370, BB415_05730, BB461_06560, BV499_05860, BZK23_04525, C2840_03950, C2844_03945, C2S07_02490, DB320_04065, DD749_04060, DD750_03440, DD754_03975, DD776_ ...Gene: AM497_02530, B0X24_07955, B0X43_08370, BB415_05730, BB461_06560, BV499_05860, BZK23_04525, C2840_03950, C2844_03945, C2S07_02490, DB320_04065, DD749_04060, DD750_03440, DD754_03975, DD776_04195, DD780_03830, EC524_01920, EC526_01900, EC558_05290, ECB91_05230, ECC12_03705, ECC36_03060, EGW00_07215, HPY1846_07480 Production host: ![]() ![]() #2: Protein | Mass: 9363.529 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The fusion protein of 2 ACP (residues 1-36 and 37-78) Source: (gene. exp.) ![]() ![]() Gene: acpP, C2S08_00655, acpP, ACM27_07645, AEY52_04040, B0X51_03260, B0X63_05470, BB389_02970, BB399_05955, BB436_06630, BFR58_08310, BGL65_04100, BGL68_01550, C2R80_01075, C2S26_06440, D2C76_07900, ...Gene: acpP, C2S08_00655, acpP, ACM27_07645, AEY52_04040, B0X51_03260, B0X63_05470, BB389_02970, BB399_05955, BB436_06630, BFR58_08310, BGL65_04100, BGL68_01550, C2R80_01075, C2S26_06440, D2C76_07900, DB320_02975, DD746_02765, DD771_02980, EC538_03680, EC543_05790, EC570_04560, EC596_03965, EC600_01035, ECC09_04825, ECC20_05115, ECC36_06460, HPF20_0453, HPF211_0816, HPF63_0811, HPF94_0692, HPK25_01094, HPMKF3_0586, HPMKM1_0566, HPMKM6_0558, HPY1198_07760, NCTC12823_00687 Production host: ![]() ![]() |
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-Non-polymers , 4 types, 201 molecules ![](data/chem/img/FMN.gif)
![](data/chem/img/SF4.gif)
![](data/chem/img/66S.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SF4.gif)
![](data/chem/img/66S.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.24 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / Details: 0.1M MES, pH 7.0, 20% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: Nov 18, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→105.479 Å / Num. obs: 46632 / % possible obs: 99.8 % / Redundancy: 13.1 % / Net I/σ(I): 10.1 |
Reflection shell | Resolution: 2.3→2.35 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4IQL Resolution: 2.31→72.62 Å / SU ML: 0.32 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 33.02 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 44.64 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.31→72.62 Å
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LS refinement shell |
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