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Open data
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Basic information
Entry | Database: PDB / ID: 70000000 | ||||||
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Title | Trans-3/4-proline-hydroxylase H11 with AKG and L-proline | ||||||
![]() | Phytanoyl-CoA dioxygenase | ||||||
![]() | HYDROLASE / L-proline / Trans / Hydroxylase / AKG / mutant | ||||||
Function / homology | phytanoyl-CoA dioxygenase activity / Phytanoyl-CoA dioxygenase / Phytanoyl-CoA dioxygenase (PhyH) / metal ion binding / 2-OXOGLUTARIC ACID / : / PROLINE / Phytanoyl-CoA dioxygenase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gong, W.G. / Yang, L.Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Trans-3/4-proline-hydroxylase H11 with AKG and L-proline Authors: Gong, W.G. / Yang, L.Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 130.8 KB | Display | ![]() |
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PDB format | ![]() | 100 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.7 MB | Display | ![]() |
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Full document | ![]() | 1.7 MB | Display | |
Data in XML | ![]() | 26.9 KB | Display | |
Data in CIF | ![]() | 38.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7dt0C ![]() 7e00C ![]() 7e05C ![]() 7e06C ![]() 7e09C ![]() 6lns S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 30108.789 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() #2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.42 Å3/Da / Density % sol: 64.01 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.7M Sodium citrate tribasic dihydrate, 0.1M Bis-Tris propane (pH 7.0) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 24, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→85.81 Å / Num. obs: 44086 / % possible obs: 94.46 % / Redundancy: 28.6 % / Rmerge(I) obs: 0.132 / Rpim(I) all: 0.025 / Rrim(I) all: 0.135 / Χ2: 3.133 / Net I/σ(I): 8.3 |
Reflection shell | Resolution: 2.14→2.18 Å / Redundancy: 28.9 % / Rmerge(I) obs: 0.529 / Mean I/σ(I) obs: 1.3 / Num. unique obs: 2262 / CC1/2: 0.981 / CC star: 0.995 / Rpim(I) all: 0.1 / Rrim(I) all: 0.538 / Χ2: 1.419 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6LNS ![]() 6lns Resolution: 2.14→85.81 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.936 / SU B: 4.364 / SU ML: 0.114 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.188 / ESU R Free: 0.173 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 123.01 Å2 / Biso mean: 39 Å2 / Biso min: 15.86 Å2
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Refinement step | Cycle: final / Resolution: 2.14→85.81 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.14→2.195 Å / Rfactor Rfree error: 0
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