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Open data
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Basic information
Entry | Database: PDB / ID: 7dqg | ||||||
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Title | Crystal structure of Salmonella phage acetyltransferase | ||||||
![]() | Putative acetyltransferase | ||||||
![]() | TRANSFERASE / Salmonella phage / SPN3US / acetyltransferase / acetyl-CoA complex | ||||||
Function / homology | Acetyltransferase (GNAT) domain / N-acetyltransferase activity / Gcn5-related N-acetyltransferase (GNAT) domain profile. / GNAT domain / Acyl-CoA N-acyltransferase / ACETYL COENZYME *A / Putative acetyltransferase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hyun, Y. / Ha, N.-C. | ||||||
![]() | ![]() Title: Crystal structure of the phage-encoded N-acetyltransferase in complex with acetyl-CoA Authors: Hyun, Y. / Oh, H.-M. / Ha, N.-C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.9 KB | Display | ![]() |
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PDB format | ![]() | 34.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 749.2 KB | Display | ![]() |
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Full document | ![]() | 750 KB | Display | |
Data in XML | ![]() | 9.2 KB | Display | |
Data in CIF | ![]() | 11.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5hgzS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21631.705 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ACO / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.42 Å3/Da / Density % sol: 64.07 % |
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Crystal grow | Temperature: 287.15 K / Method: vapor diffusion, hanging drop Details: 0.4 M sodium malonate pH 6.0, 0.1 M MES monohydrate pH 6.0, 0.5% (w/v) PEG 10000 |
-Data collection
Diffraction | Mean temperature: 100.15 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 31, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97941 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 15793 / % possible obs: 98.7 % / Redundancy: 21.1 % / Biso Wilson estimate: 28.62 Å2 / CC1/2: 0.994 / CC star: 0.999 / Rmerge(I) obs: 0.077 / Rpim(I) all: 0.017 / Rrim(I) all: 0.091 / Net I/σ(I): 36.7 |
Reflection shell | Resolution: 2.2→2.24 Å / Rmerge(I) obs: 0.324 / Num. unique obs: 718 / CC1/2: 0.959 / CC star: 0.989 / Rpim(I) all: 0.067 / Rrim(I) all: 0.283 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5HGZ Resolution: 2.21→40.22 Å / SU ML: 0.2629 / Cross valid method: FREE R-VALUE / σ(F): 1.79 / Phase error: 20.1898 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.29 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.21→40.22 Å
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Refine LS restraints |
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LS refinement shell |
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