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- PDB-7dmp: Mouse radial spoke complex -

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Basic information

Entry
Database: PDB / ID: 7dmp
TitleMouse radial spoke complex
Components
  • Radial spoke head 1 homolog
  • Radial spoke head protein 4 homolog A
  • Radial spoke head protein 9 homolog
KeywordsSTRUCTURAL PROTEIN / Cilia / Complex / Signal transducer.
Function / homology
Function and homology information


radial spoke assembly / maintenance of ciliary planar beating movement pattern / radial spoke head 1 / radial spoke head 2 / radial spoke head 3 / axonemal central apparatus assembly / outer dense fiber / radial spoke head / radial spoke / mating ...radial spoke assembly / maintenance of ciliary planar beating movement pattern / radial spoke head 1 / radial spoke head 2 / radial spoke head 3 / axonemal central apparatus assembly / outer dense fiber / radial spoke head / radial spoke / mating / epithelial cilium movement involved in extracellular fluid movement / kinocilium / cilium movement involved in cell motility / 9+2 motile cilium / cilium movement / axoneme assembly / flagellated sperm motility / meiotic spindle / motile cilium / axoneme / spermatid development / sperm flagellum / condensed nuclear chromosome / meiotic cell cycle / establishment of localization in cell / extracellular region / cytoplasm
Similarity search - Function
Radial spokehead-like protein / Radial spokehead-like protein / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / MORN motif / MORN repeat
Similarity search - Domain/homology
Radial spoke head protein 4 homolog A / Radial spoke head 1 homolog / Radial spoke head protein 9 homolog
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsZheng, W. / Cong, Y.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Proc Natl Acad Sci U S A / Year: 2021
Title: Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM.
Authors: Wei Zheng / Fan Li / Zhanyu Ding / Hao Liu / Lei Zhu / Cong Xu / Jiawei Li / Qi Gao / Yanxing Wang / Zhenglin Fu / Chao Peng / Xiumin Yan / Xueliang Zhu / Yao Cong /
Abstract: The radial spoke (RS) heads of motile cilia and flagella contact projections of the central pair (CP) apparatus to coordinate motility, but the morphology is distinct for protozoa and metazoa. Here ...The radial spoke (RS) heads of motile cilia and flagella contact projections of the central pair (CP) apparatus to coordinate motility, but the morphology is distinct for protozoa and metazoa. Here we show the murine RS head is compositionally distinct from that of Our reconstituted murine RS head core complex consists of Rsph1, Rsph3b, Rsph4a, and Rsph9, lacking Rsph6a and Rsph10b, whose orthologs exist in the protozoan RS head. We resolve its cryo-electron microscopy (cryo-EM) structure at 3.2-Å resolution. Our atomic model further reveals a twofold symmetric brake pad-shaped structure, in which Rsph4a and Rsph9 form a compact body extended laterally with two long arms of twisted Rsph1 β-sheets and potentially connected dorsally via Rsph3b to the RS stalk. Furthermore, our modeling suggests that the core complex contacts the periodic CP projections either rigidly through its tooth-shaped Rsph4a regions or elastically through both arms for optimized RS-CP interactions and mechanosignal transduction.
History
DepositionDec 5, 2020Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 21, 2021Provider: repository / Type: Initial release
Revision 1.1Mar 27, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Assembly

Deposited unit
B: Radial spoke head protein 4 homolog A
b: Radial spoke head protein 4 homolog A
A: Radial spoke head 1 homolog
a: Radial spoke head 1 homolog
C: Radial spoke head protein 9 homolog
c: Radial spoke head protein 9 homolog


Theoretical massNumber of molelcules
Total (without water)291,5976
Polymers291,5976
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Radial spoke head protein 4 homolog A / Radial spoke protein head 4a / Radial spoke head-like protein 3


Mass: 80214.102 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Rsph4a, Rshl3 / Production host: Homo sapiens (human) / References: UniProt: Q8BYM7
#2: Protein Radial spoke head 1 homolog / Male meiotic metaphase chromosome-associated acidic protein / Meichroacidin / Testis-specific gene A2 protein


Mass: 34218.254 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Rsph1, Tsga2 / Production host: Homo sapiens (human) / References: UniProt: Q8VIG3
#3: Protein Radial spoke head protein 9 homolog / radial spoke protein head 9


Mass: 31365.902 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Rsph9 / Production host: Homo sapiens (human) / References: UniProt: Q9D9V4

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Mouse radial spoke head complex / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Mus musculus (house mouse)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 58 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 302573 / Symmetry type: POINT

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