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- SASDC72: Alcohol dehydrogenase from bifunctional alcohol/aldehyde dehydrog... -

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Basic information

Entry
Database: SASBDB / ID: SASDC72
SampleAlcohol dehydrogenase from bifunctional alcohol/aldehyde dehydrogenase (P0A9Q8)
  • Aldehyde-alcohol dehydrogenase (protein), AdhE, Escherichia coli O157:H7
Function / homology
Function and homology information


acetaldehyde dehydrogenase (acetylating) / acetaldehyde dehydrogenase (acetylating) activity / alcohol metabolic process / carbon utilization / alcohol dehydrogenase (NAD+) activity / alcohol dehydrogenase / metal ion binding
Similarity search - Function
Bifunctional aldehyde-alcohol dehydrogenase / Bifunctional aldehyde-alcohol dehydrogenase, C-terminal domain / Iron-type alcohol dehydrogenase-like / Iron-containing alcohol dehydrogenases signature 2. / Iron-containing alcohol dehydrogenases signature 1. / Alcohol dehydrogenase, iron-type, conserved site / Alcohol dehydrogenase, iron-type/glycerol dehydrogenase GldA / Iron-containing alcohol dehydrogenase / Aldehyde dehydrogenase domain / Aldehyde dehydrogenase family ...Bifunctional aldehyde-alcohol dehydrogenase / Bifunctional aldehyde-alcohol dehydrogenase, C-terminal domain / Iron-type alcohol dehydrogenase-like / Iron-containing alcohol dehydrogenases signature 2. / Iron-containing alcohol dehydrogenases signature 1. / Alcohol dehydrogenase, iron-type, conserved site / Alcohol dehydrogenase, iron-type/glycerol dehydrogenase GldA / Iron-containing alcohol dehydrogenase / Aldehyde dehydrogenase domain / Aldehyde dehydrogenase family / Aldehyde dehydrogenase, C-terminal / Aldehyde dehydrogenase, N-terminal / Aldehyde/histidinol dehydrogenase
Similarity search - Domain/homology
Bifunctional aldehyde-alcohol dehydrogenase AdhE
Similarity search - Component
Biological speciesEscherichia coli O157:H7 (bacteria)
Contact author
  • Liyana Azmi (University of Glasgow, Glasgow, Scotland, UK)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1157
Type: dummy / Software: (r9217) / Radius of dummy atoms: 3.50 A / Symmetry: P2 / Chi-square value: 1.847 / P-value: 0.213700
Search similar-shape structures of this assembly by Omokage search (details)
Model #1262
Type: dummy / Software: (5.0 r9217) / Radius of dummy atoms: 3.50 A / Symmetry: P2 / Chi-square value: 1.847 / P-value: 0.213700
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Alcohol dehydrogenase from bifunctional alcohol/aldehyde dehydrogenase (P0A9Q8)
Specimen concentration: 10 mg/ml
BufferName: 20 mM Tris 400 mM NaCl 5% v/v glycerol / pH: 7.5
Entity #583Name: AdhE / Type: protein / Description: Aldehyde-alcohol dehydrogenase / Formula weight: 48.33 / Num. of mol.: 2 / Source: Escherichia coli O157:H7 / References: UniProt: P0A9Q8
Sequence: MLWHKLPKSI YFRRGSLPIA LDEVITDGHK RALIVTDRFL FNNGYADQIT SVLKAAGVET EVFFEVEADP TLSIVRKGAE LANSFKPDVI IALGGGSPMD AAKIMWVMYE HPETHFEELA LRFMDIRKRI YKFPKMGVKA KMIAVTTTSG TGSEVTPFAV VTDDATGQKY ...Sequence:
MLWHKLPKSI YFRRGSLPIA LDEVITDGHK RALIVTDRFL FNNGYADQIT SVLKAAGVET EVFFEVEADP TLSIVRKGAE LANSFKPDVI IALGGGSPMD AAKIMWVMYE HPETHFEELA LRFMDIRKRI YKFPKMGVKA KMIAVTTTSG TGSEVTPFAV VTDDATGQKY PLADYALTPD MAIVDANLVM DMPKSLCAFG GLDAVTHAME AYVSVLASEF SDGQALQALK LLKEYLPASY HEGSKNPVAR ERVHSAATIA GIAFANAFLG VCHSMAHKLG SQFHIPHGLA NALLICNVIR YNANDNPTKQ TAFSQYDRPQ ARRRYAEIAD HLGLSAPGDR TAAKIEKLLA WLETLKAELG IPKSIREAGV QEADFLANVD KLSEDAFDDQ CTGANPRYPL ISELKQILLD TYYGRDYVEG ETAAKKEAAP AKAEKKAKKS A

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.04 mm
DetectorName: Pilatus 2M
Scan
Title: Alcohol dehydrogenase from bifunctional alcohol/aldehyde dehydrogenase (P0A9Q8)
Measurement date: Nov 27, 2015 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 1 sec. / Number of frames: 131 / Unit: 1/A /
MinMax
Q0.0045 0.4017
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 992 /
MinMax
Q0.0140907 0.286488
P(R) point1 992
R0 168.7
Result
Type of curve: single_conc
ExperimentalPorodEstimatedEstimated method
MW91.4 kDa---
Volume-146 nm3178 DAMMIN

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.0627 0.0001 0.061 7.0E-5
Radius of gyration, Rg3.55 nm0.23 3.24 nm0.06

MinMax
D-16.87
Guinier point36 130

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