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- PDB-7dkh: Crystal structure of the Ctr9/Paf1/Cdc73/Rtf1 quaternary complex -

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基本情報

登録情報
データベース: PDB / ID: 7dkh
タイトルCrystal structure of the Ctr9/Paf1/Cdc73/Rtf1 quaternary complex
要素
  • Cell division control protein 73
  • RNA polymerase II-associated protein 1
  • RNA polymerase-associated protein CTR9
  • RNA polymerase-associated protein RTF1
キーワードGENE REGULATION / Paf1 complex / H2B mono-ubiquitination / Rad6/Bre1 / ubiquitin discharge
機能・相同性
機能・相同性情報


: / : / : / snoRNA transcription by RNA polymerase II / : / : / positive regulation of transcription elongation by RNA polymerase I / regulation of transcription-coupled nucleotide-excision repair / sno(s)RNA 3'-end processing / regulation of transcription initiation by RNA polymerase II ...: / : / : / snoRNA transcription by RNA polymerase II / : / : / positive regulation of transcription elongation by RNA polymerase I / regulation of transcription-coupled nucleotide-excision repair / sno(s)RNA 3'-end processing / regulation of transcription initiation by RNA polymerase II / meiotic DNA double-strand break formation / RNA polymerase II C-terminal domain phosphoserine binding / triplex DNA binding / global genome nucleotide-excision repair / Cdc73/Paf1 complex / negative regulation of DNA recombination / rDNA heterochromatin formation / mRNA 3'-end processing / negative regulation of mitophagy / : / RNA polymerase II complex binding / transcription by RNA polymerase I / transcription by RNA polymerase III / transcription elongation by RNA polymerase I / transcription elongation by RNA polymerase II / positive regulation of transcription elongation by RNA polymerase II / DNA-templated transcription termination / transcription coregulator activity / euchromatin / rRNA processing / RNA polymerase II-specific DNA-binding transcription factor binding / chromatin binding / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / DNA binding / RNA binding / nucleus
類似検索 - 分子機能
Plus-3 domain / Plus3-like superfamily / Plus-3 domain / Plus3 domain profile. / Short conserved domain in transcriptional regulators. / Cdc73/Parafibromin / RNA polymerase-associated protein Ctr9 / Cell division control protein 73, C-terminal / Cell division control protein 73, C-terminal domain superfamily / RNA pol II accessory factor, Cdc73 family, C-terminal ...Plus-3 domain / Plus3-like superfamily / Plus-3 domain / Plus3 domain profile. / Short conserved domain in transcriptional regulators. / Cdc73/Parafibromin / RNA polymerase-associated protein Ctr9 / Cell division control protein 73, C-terminal / Cell division control protein 73, C-terminal domain superfamily / RNA pol II accessory factor, Cdc73 family, C-terminal / RNA polymerase II associated factor Paf1 / Paf1 / Tetratricopeptide repeat / Tetratricopeptide repeat / TPR repeat region circular profile. / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily
類似検索 - ドメイン・相同性
RNA polymerase II-associated protein 1 / RNA polymerase-associated protein RTF1 / RNA polymerase-associated protein CTR9 / Cell division control protein 73
類似検索 - 構成要素
生物種Saccharomyces cerevisiae (パン酵母)
手法X線回折 / シンクロトロン / 単波長異常分散 / 解像度: 2.9 Å
データ登録者Chen, F.L. / Liu, B.B. / Guo, L. / Li, D.F. / Zhou, H. / Long, J.F.
資金援助 中国, 2件
組織認可番号
National Natural Science Foundation of China (NSFC)31670758 中国
National Natural Science Foundation of China (NSFC)31870750 中国
引用ジャーナル: J.Mol.Biol. / : 2021
タイトル: Crystal Structure of the Core Module of the Yeast Paf1 Complex.
著者: Chen, F.L. / Liu, B.B. / Zeng, J.W. / Guo, L. / Ge, X. / Feng, W. / Li, D.F. / Zhou, H. / Long, J.F.
履歴
登録2020年11月24日登録サイト: PDBJ / 処理サイト: PDBJ
改定 1.02021年11月24日Provider: repository / タイプ: Initial release
改定 1.12022年1月5日Group: Database references / カテゴリ: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

ダウンロードとリンク

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集合体

登録構造単位
A: RNA polymerase-associated protein CTR9
B: RNA polymerase II-associated protein 1
C: Cell division control protein 73
D: RNA polymerase-associated protein RTF1
E: RNA polymerase-associated protein CTR9
F: RNA polymerase II-associated protein 1
G: Cell division control protein 73
H: RNA polymerase-associated protein RTF1
I: RNA polymerase-associated protein CTR9
J: RNA polymerase II-associated protein 1
K: Cell division control protein 73
L: RNA polymerase-associated protein RTF1


分子量 (理論値)分子数
合計 (水以外)420,73012
ポリマ-420,73012
非ポリマー00
00
1
A: RNA polymerase-associated protein CTR9
B: RNA polymerase II-associated protein 1
C: Cell division control protein 73
D: RNA polymerase-associated protein RTF1


分子量 (理論値)分子数
合計 (水以外)140,2434
ポリマ-140,2434
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
Buried area21220 Å2
ΔGint-135 kcal/mol
Surface area52890 Å2
手法PISA
2
E: RNA polymerase-associated protein CTR9
F: RNA polymerase II-associated protein 1
G: Cell division control protein 73
H: RNA polymerase-associated protein RTF1


分子量 (理論値)分子数
合計 (水以外)140,2434
ポリマ-140,2434
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
Buried area20890 Å2
ΔGint-134 kcal/mol
Surface area53270 Å2
手法PISA
3
I: RNA polymerase-associated protein CTR9
J: RNA polymerase II-associated protein 1
K: Cell division control protein 73
L: RNA polymerase-associated protein RTF1


分子量 (理論値)分子数
合計 (水以外)140,2434
ポリマ-140,2434
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
Buried area20960 Å2
ΔGint-137 kcal/mol
Surface area53100 Å2
手法PISA
単位格子
Length a, b, c (Å)190.195, 190.195, 216.423
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number152
Space group name H-MP3121
Space group name HallP312"
Symmetry operation#1: x,y,z
#2: -y,x-y,z+1/3
#3: -x+y,-x,z+2/3
#4: x-y,-y,-z+2/3
#5: -x,-x+y,-z+1/3
#6: y,x,-z

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要素

#1: タンパク質 RNA polymerase-associated protein CTR9 / Centromere-binding factor 1-dependent protein 1 / Cln three-requiring protein 9


分子量: 113746.820 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
遺伝子: CTR9, CDP1, YOL145C / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P89105
#2: タンパク質 RNA polymerase II-associated protein 1 / Protein PAF1


分子量: 11956.307 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
遺伝子: PAF1, YBR279W, YBR2016 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P38351
#3: タンパク質 Cell division control protein 73 / RNA polymerase-associated protein CDC73


分子量: 6333.072 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
遺伝子: CDC73, YLR418C / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q06697
#4: タンパク質 RNA polymerase-associated protein RTF1


分子量: 8207.274 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
遺伝子: RTF1, CSL3, YGL244W, HRA458 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P53064
研究の焦点であるリガンドがあるかY

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実験情報

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実験

実験手法: X線回折 / 使用した結晶の数: 1

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試料調製

結晶マシュー密度: 2.71 Å3/Da / 溶媒含有率: 54.57 %
結晶化温度: 289 K / 手法: 蒸気拡散法, ハンギングドロップ法 / 詳細: 8% tascimate, pH 6.0, 12% PEG 3350

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データ収集

回折平均測定温度: 97 K / Serial crystal experiment: N
放射光源由来: シンクロトロン / サイト: SSRF / ビームライン: BL19U1 / 波長: 0.97791 Å
検出器タイプ: DECTRIS PILATUS3 6M / 検出器: PIXEL / 日付: 2018年11月3日
放射プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長波長: 0.97791 Å / 相対比: 1
反射解像度: 2.9→48.96 Å / Num. obs: 100181 / % possible obs: 99.9 % / 冗長度: 31.2 % / Biso Wilson estimate: 73.48 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.173 / Rpim(I) all: 0.044 / Rrim(I) all: 0.178 / Net I/σ(I): 17
反射 シェル解像度: 2.9→3.004 Å / Rmerge(I) obs: 1.756 / Mean I/σ(I) obs: 2.5 / Num. unique obs: 9841 / CC1/2: 0.794 / Rpim(I) all: 0.455 / Rrim(I) all: 1.815

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解析

ソフトウェア
名称バージョン分類
PHENIX1.14_3260精密化
XDSデータ削減
XDSデータスケーリング
PHENIX位相決定
精密化構造決定の手法: 単波長異常分散 / 解像度: 2.9→48.96 Å / SU ML: 0.4021 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 28.0631 / 立体化学のターゲット値: CDL v1.2
Rfactor反射数%反射
Rfree0.2478 5119 5.11 %
Rwork0.1926 95013 -
obs0.1954 100132 99.81 %
溶媒の処理減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL
原子変位パラメータBiso mean: 90.01 Å2
精密化ステップサイクル: LAST / 解像度: 2.9→48.96 Å
タンパク質核酸リガンド溶媒全体
原子数27198 0 0 0 27198
拘束条件
Refine-IDタイプDev ideal
X-RAY DIFFRACTIONf_bond_d0.004327674
X-RAY DIFFRACTIONf_angle_d0.936137277
X-RAY DIFFRACTIONf_chiral_restr0.04914167
X-RAY DIFFRACTIONf_plane_restr0.00624768
X-RAY DIFFRACTIONf_dihedral_angle_d23.688910648
LS精密化 シェル
解像度 (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.9-2.930.36721620.31013065X-RAY DIFFRACTION96.76
2.93-2.970.32481660.26983150X-RAY DIFFRACTION100
2.97-30.33421610.27023136X-RAY DIFFRACTION100
3-3.040.33731670.27463126X-RAY DIFFRACTION100
3.04-3.080.35491820.27793127X-RAY DIFFRACTION100
3.08-3.120.39471890.26583128X-RAY DIFFRACTION100
3.12-3.170.31932120.26863143X-RAY DIFFRACTION100
3.17-3.220.36521580.26843112X-RAY DIFFRACTION100
3.22-3.270.28751310.2543181X-RAY DIFFRACTION100
3.27-3.320.30221550.24623171X-RAY DIFFRACTION100
3.32-3.380.34091770.24453142X-RAY DIFFRACTION100
3.38-3.440.2691690.22723153X-RAY DIFFRACTION99.97
3.44-3.50.29121670.22953145X-RAY DIFFRACTION99.85
3.5-3.580.30351540.21423164X-RAY DIFFRACTION99.91
3.58-3.650.27061860.21433174X-RAY DIFFRACTION99.97
3.65-3.740.26661690.20553130X-RAY DIFFRACTION99.94
3.74-3.830.28071640.19163164X-RAY DIFFRACTION99.97
3.83-3.940.21871520.18133178X-RAY DIFFRACTION100
3.94-4.050.23431940.1783149X-RAY DIFFRACTION100
4.05-4.180.22181640.17133178X-RAY DIFFRACTION99.97
4.18-4.330.19771780.17173167X-RAY DIFFRACTION99.97
4.33-4.50.25211990.1693146X-RAY DIFFRACTION99.94
4.5-4.710.21181810.15473151X-RAY DIFFRACTION100
4.71-4.960.20331890.15133159X-RAY DIFFRACTION100
4.96-5.270.2321870.17093193X-RAY DIFFRACTION100
5.27-5.670.2741610.19463194X-RAY DIFFRACTION99.97
5.67-6.240.25621370.20483251X-RAY DIFFRACTION99.97
6.24-7.150.25391620.18163243X-RAY DIFFRACTION99.97
7.15-8.990.17491800.15263259X-RAY DIFFRACTION99.88
8.99-48.960.1871660.15873334X-RAY DIFFRACTION98.29
精密化 TLS

手法: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
15.27674820412-1.81129239866-0.687488033344.244840300973.589459356027.662439306090.1393199623360.3753733100540.04782727120540.252222911284-0.4621901587720.827541784170.504833776002-1.257370582760.2934791554170.621066969984-0.1026067690230.1220386791690.721419750149-0.1575449404011.31079788277-15.663585675771.547207090415.6300989559
22.047177823050.387624866150.1799738053353.186297676040.816795997731.231758437280.01804568996660.194451302997-0.5461297160460.132831454584-0.121786413550.3571834803140.199740228392-0.06436422381180.09750712704990.381721977490.03342076771240.0398763716960.438316014343-0.06517903714770.412329376718.8037336922196.265718877225.8377960083
32.783170891072.09538630479-0.09783631512254.952754968670.4766082379451.44748031148-0.1273724417860.4340035677210.439733871215-0.2930754192930.218730120736-0.263104642467-0.294637960120.267990515641-0.1069875524150.341505552333-0.0419189229222-0.0523100793090.5542257076950.1538778917730.54286404394540.926425327138.07217706933.9792462851
48.492737770880.358905142151.064656624221.009194280130.5240282097731.00223370859-0.0149315391509-0.2954116536390.5643795514030.430178895690.164722305965-0.3144590451690.1517259233720.181304850905-0.1613516534560.609007471363-0.0697015091465-0.1157151433260.510178767527-0.04358748784650.55279237880561.540177901140.05317164959.8822140824
51.24025909786-0.841972556918-1.579602900364.603082116891.543364042632.3616000515-0.418505103952-0.5226744492090.614474837950.5118265284920.833580086561-0.8274405197760.1554229952820.453166645373-0.4073606755940.724041012790.116823798618-0.4149708936790.933594934566-0.3873084262271.0155540211694.8007855669141.8930790666.3439746946
65.103247113065.296391658323.297768720558.792288321342.303700874622.42227030345-1.00033702737-1.201389881192.600160161270.4571135038360.1236362088040.370109726385-0.272023299139-0.2391952434230.6211234154510.716491881525-0.1684427698050.02657180607680.79431451406-0.2137017937860.76170683747765.8096705466143.05380304363.6867935236
72.754624345562.542932150055.004370193722.279024363434.451678710318.8954942839-0.7346393826210.4172103697130.233949574365-0.03117081818640.1038992795730.666645151124-0.1407831695381.314467954040.4135189657060.405826035684-0.138216813588-0.07761203416190.694514673340.1358170842880.80668874792546.506503314141.76600850742.9296743555
82.012344700962.1647494468-0.8306087822342.13673847707-0.9223320135910.347865542577-0.9474724658710.629572125921.10266246202-1.417036972680.5013184354160.881240638837-0.1135619873520.04946595472420.3163055024280.531242855335-0.0827195074879-0.1277251250950.5495513385010.1253039763120.48214110641226.56922077127.60249285925.8298470902
97.515268074965.425601025732.563541888477.041169025812.678809953921.362574982160.4257486553550.603610289797-0.5675966132170.976298800896-0.04332381624260.2753343696250.3781246438280.126798389345-0.363254200730.442430357629-0.001533445156690.01768313682140.499549828663-0.1262782778190.38537132149611.535326029198.808464500723.0688721079
104.459804883725.255929313022.833015641886.087754691853.306573493692.082863279260.4979225512490.290435290263-1.01226302561-0.28853924179-0.447429088932-0.2722070677420.5051474781640.0519552052959-0.02775751810710.799935539874-0.05173534395350.134628397860.59338540131-0.1123668858151.26830961898-3.5448316319367.883920318120.8276853704
115.63177180321-0.768276322168-0.9672733357127.316510165450.9054245022758.77259697863-0.4348855766490.377105877427-2.07166430463-0.3041617943551.02048462442-1.465746513650.8454682087551.09685846716-0.6438654393280.6495523236290.224920026498-0.08921904992340.767897066714-0.3374959117921.3526056086919.606993802873.240536883719.780541494
125.039982575133.61977912517-2.577487629133.19734887577-2.217072912217.151459087610.910313563322-0.0561891409071.80548700246-1.692423565390.3713928830510.999345748223-0.246121842877-1.43516399788-1.038133309351.204727377340.161211764420.05653568918861.135148867620.7727445143742.0421330689330.2408673742157.93529641132.35315584
133.443240325744.03005826853-3.800441626694.72390891202-4.441284909454.178971680670.1260151544460.707205110754-0.8308367816770.5443911438080.08054006920870.0973614754414-1.28038937609-0.68654675854-0.3972630988660.914362254736-0.00902474670126-0.1380524610830.9257733081910.251982311330.96877869069629.6726554628146.31580309626.6811288233
147.75354270623-0.6435902479874.758959612841.1162002361-0.7721596443.052387091670.04861683173660.3235010171070.6770786342480.298837054414-0.3067327291580.243279688794-0.01186508366460.235512959170.152729668460.477099761472-0.0935246003012-0.006328542616050.452650304354-0.0165068221120.57087278341940.0327985076133.63708994142.534237668
156.311965637852.64750205362.132984363044.886396947864.771156002954.722148633330.0242715085718-1.1259531439-0.4124620713312.4993044197-1.135450616650.8385069150271.34623563735-0.117038608950.8481321588920.98403687499-0.1841102799840.05369707153830.785282311320.1341992404950.67722788599741.955690684128.31148845365.3980079504
166.50262169863-6.10736951692-5.040440449755.769060701934.75005100133.903597413960.09703619613-2.321934364520.066801220662.54536322318-0.3838536984970.8074197956551.312273674380.3148350869940.2151428922721.19447791477-0.4564954560210.0751510385521.33443232984-0.01161867040640.67877361294243.7019291046136.08185600475.2403225273
173.64339275794-1.937677612-1.08226398391.0329277320.5806846277530.3123044483030.0922487089698-0.5848060171880.739887252179-1.3158358949-0.111714254836-1.20352836907-0.05227189922030.745607000673-0.04726038928611.124032825810.1063199335720.2329461689481.395096084-0.121847720741.4204456521358.752470336688.76745326118.39753903246
185.22624876298-7.411767339440.5877250069822.0194098551-0.8495800187470.5746994526470.656317384476-2.655626977690.222173692638-0.8779017886830.346485624789-0.8681540679630.3487404018261.76664226141-0.8179224619060.702765264460.08057432180990.02428495659031.37369370441-0.06192939265291.0974886464141.847659038100.29083241611.9161389152
195.171213880293.562897649632.214775172023.384428977911.64354942585.64009002444-0.1292257591091.679471336340.258007669739-0.7926634055810.1214841276050.220142681113-0.277152752984-0.0169332973610.02580119635990.6750475354410.111964055343-0.04007019048930.896370870856-0.03658452995950.35599593061616.2912831707110.2370229929.22979449579
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367.436871485-1.943029500213.22780307127.771375689325.010762771469.036419571250.251209040598-0.7029515990450.9579425483080.8359771007970.04570262836110.4828293202980.0919829000768-0.740632076196-0.2950575902250.638782234505-0.1799649122760.2223155388850.465585886236-0.05898249381720.60973386647486.7052063367119.63248661156.7280548234
373.56135471058-2.010929517795.67024375031.24843401113-3.204793630689.00264212125-0.211414049056-1.992401231250.4799073634222.587725759060.0246720094308-0.577313715561-1.37314217949-1.867734416430.17880693021.87394715350.00406028484904-0.07235195825411.05850614924-0.1065807068410.82453744897393.6504608445115.9168721274.8092210422
382.92493660979-2.22414318599-4.294445132859.81483124859-0.6146061102898.1282464499-0.03718425028041.39627563314-1.737216555110.391979988624-0.735164973119-0.07515365361290.900360774541-0.9150553144280.5190511512732.122571902010.06105734374160.07559432617421.59666019015-0.521467856131.7049483495543.0020708819128.7568830778.6069569301
398.228805013290.169288721039-3.145923787361.57956847859-1.33390189232.199607369620.6931398587590.744911529769-1.338218947871.37182868221-0.1878140693010.03712011391160.5881256364-0.684204324626-0.3130780834821.15111733992-0.296877100054-0.1768763172111.003895555330.06251672003771.102760466560.7638666331135.76913797312.3845557245
408.52215309671.769918412081.416766259174.15471362157-3.44485952883.88032459928-0.7001792903220.524633991545-0.251386458256-1.877632720640.4080668188230.1016821123490.5975981691170.2641917415710.133280143430.804385055689-0.117277220080.002730558973530.5998523711280.002915372251670.53304297691680.1370383729145.89593775312.6820341346
410.4791498056190.903680426222-1.166492560037.77535676898-3.491905462823.13006038792-0.8019116731681.455727346210.408638080315-2.501653779151.46801448534-0.0167187168707-0.8831279001450.870860352988-0.5840930537281.2123710523-0.33975804390.08572793788671.034039173020.3024993902070.8281342550284.8883414166163.3618129684.73044357801
426.138156407360.604176767445-1.922500982345.18902061917-1.872446315086.05702238333-0.198328249222-0.206779907528-1.20653940075-0.543048108421-0.119395210705-0.2866773690980.9472744449190.8577560026760.2921008821080.7002150792030.1579898669520.22118153530.5998302600530.07705192520661.05994579119136.08269629164.984914357517.2878963056
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476.215140346386.00216360359-1.373613173136.47357842035-2.16172934321.726388262360.373786254-0.02993599475691.406588161171.941958666560.4077044663351.86604238061-0.243024845031-0.222505428083-0.7271316468350.9446001320360.06944617424930.3454251591810.816677906454-0.009262936633720.74270017227819.2753131504135.70438848265.1065334228
488.38568812136-2.099064546845.275465390719.93278855049-3.887779424064.491946831460.406498336729-0.375265481147-0.5837113003341.695969272160.698589419615-0.03213813200220.464427384746-0.123337499053-0.8927067758461.079175752290.0624307877288-0.1481915740280.6304107361090.2034717278270.68129849307830.300726008998.738356696762.1164075733
497.45691618347-0.860678570056-0.4098464345991.141947457530.5783075773062.70384064933-0.2163365333820.991376200920.103229599750.110040232636-0.7087693673610.223414893671-0.260758225048-0.2773918992250.8448833275950.5294351334240.129620917067-0.03555406740690.5475449992070.05015575841151.2081520338348.072646047181.491714926336.7473400855
504.56801170829-3.267863214934.269768455135.60598980045-0.2133787924926.52006156191-0.4526472585742.9274350954-0.997690303278-0.283043892322-0.3809563143591.01933513748-0.284974807084-0.8629941250930.6860843550310.596577094875-0.0811133465613-0.03495247559910.982366460483-0.1057764782930.50838461134572.097457363172.667166630624.1413122691
516.28154893477-0.107110985666-0.3227414875611.09251967350.3496915175920.5284762525990.3123073946780.126724457326-0.151621736039-0.135107643125-0.204365952517-0.353981213429-0.01713365588860.0905602412181-0.1450547467780.4951609677580.0005848301101430.1010126537940.4575450270040.06064015652410.379838764143103.86075861975.302210242523.0120833154
525.37515280401-6.81634689441-1.379858700359.026875721531.052269711762.442799843020.325455682013-0.7331803114771.32755218565-0.212764048367-0.274951014742-1.93974731185-0.214088822811.220273131480.01372695568070.516449154978-0.1037248131370.07228196173150.6542000116370.04500173951390.924455843899135.79629909378.778374795623.3084596382
536.55204357062-0.1769423965290.4566249855642.64182916854-0.6217722145817.896677953270.7457714763930.4800273564822.59761853910.12575839865-0.282207279277-0.619886392037-1.35347223544-0.0662244915385-0.4385181952660.774727337107-0.1371170085170.2386898972130.443790782352-0.04597247207731.14598097723117.70843733595.177393105621.2895400309
543.00232461017-1.221344573582.202938857983.41383936028-2.993764764293.13428723858-0.4039324480670.145648108973-0.803701251709-0.8485075699710.5924724061150.9974127931160.658939471023-0.139446804395-0.2683322443570.657186214411-0.0263844507321-0.1325451004860.652758372215-0.2229525304871.4196722033643.482474106364.459855200226.5577579808
551.89307436036-1.09245204181-2.86396928542.483095269431.80799408777.13004643330.3503154305230.623667612241-0.54016088430.0272828386669-0.132754773514-0.4072774223750.769764766017-0.391950651065-0.1636022917630.6221343385410.12893544762-0.1005377060250.6357809871110.05732343845661.2298641691151.610574824675.008329862433.8010785345
561.89050193835-0.2992653446760.09354102642612.741066273221.075390524532.79751858191-0.43261274712-0.374438902283-0.07905686316561.23194671643-0.517139218749-1.08327626142-0.1072747433571.353945139890.7645444729191.05222727269-0.0313796983259-0.5379818328210.7276178437140.2252637654991.2166120341652.860386890185.469702949961.259091973
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精密化 TLSグループ
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 10 through 133 )
2X-RAY DIFFRACTION2chain 'A' and (resid 134 through 430 )
3X-RAY DIFFRACTION3chain 'A' and (resid 431 through 646 )
4X-RAY DIFFRACTION4chain 'A' and (resid 647 through 834 )
5X-RAY DIFFRACTION5chain 'A' and (resid 835 through 922 )
6X-RAY DIFFRACTION6chain 'B' and (resid 5 through 14 )
7X-RAY DIFFRACTION7chain 'B' and (resid 15 through 24 )
8X-RAY DIFFRACTION8chain 'B' and (resid 25 through 39 )
9X-RAY DIFFRACTION9chain 'B' and (resid 40 through 65 )
10X-RAY DIFFRACTION10chain 'B' and (resid 66 through 84 )
11X-RAY DIFFRACTION11chain 'B' and (resid 85 through 103 )
12X-RAY DIFFRACTION12chain 'C' and (resid 2 through 7 )
13X-RAY DIFFRACTION13chain 'C' and (resid 8 through 15 )
14X-RAY DIFFRACTION14chain 'C' and (resid 16 through 35 )
15X-RAY DIFFRACTION15chain 'C' and (resid 36 through 45 )
16X-RAY DIFFRACTION16chain 'C' and (resid 46 through 53 )
17X-RAY DIFFRACTION17chain 'D' and (resid 18 through 26 )
18X-RAY DIFFRACTION18chain 'D' and (resid 27 through 43 )
19X-RAY DIFFRACTION19chain 'D' and (resid 44 through 68 )
20X-RAY DIFFRACTION20chain 'E' and (resid 10 through 233 )
21X-RAY DIFFRACTION21chain 'E' and (resid 234 through 454 )
22X-RAY DIFFRACTION22chain 'E' and (resid 455 through 600 )
23X-RAY DIFFRACTION23chain 'E' and (resid 601 through 646 )
24X-RAY DIFFRACTION24chain 'E' and (resid 647 through 922 )
25X-RAY DIFFRACTION25chain 'F' and (resid 5 through 15 )
26X-RAY DIFFRACTION26chain 'F' and (resid 16 through 39 )
27X-RAY DIFFRACTION27chain 'F' and (resid 40 through 55 )
28X-RAY DIFFRACTION28chain 'F' and (resid 56 through 60 )
29X-RAY DIFFRACTION29chain 'F' and (resid 61 through 65 )
30X-RAY DIFFRACTION30chain 'F' and (resid 66 through 80 )
31X-RAY DIFFRACTION31chain 'F' and (resid 81 through 85 )
32X-RAY DIFFRACTION32chain 'F' and (resid 86 through 103 )
33X-RAY DIFFRACTION33chain 'G' and (resid 3 through 7 )
34X-RAY DIFFRACTION34chain 'G' and (resid 8 through 12 )
35X-RAY DIFFRACTION35chain 'G' and (resid 13 through 24 )
36X-RAY DIFFRACTION36chain 'G' and (resid 25 through 45 )
37X-RAY DIFFRACTION37chain 'G' and (resid 46 through 53 )
38X-RAY DIFFRACTION38chain 'H' and (resid 18 through 26 )
39X-RAY DIFFRACTION39chain 'H' and (resid 27 through 43 )
40X-RAY DIFFRACTION40chain 'H' and (resid 44 through 59 )
41X-RAY DIFFRACTION41chain 'H' and (resid 60 through 68 )
42X-RAY DIFFRACTION42chain 'I' and (resid 10 through 133 )
43X-RAY DIFFRACTION43chain 'I' and (resid 134 through 430 )
44X-RAY DIFFRACTION44chain 'I' and (resid 431 through 600 )
45X-RAY DIFFRACTION45chain 'I' and (resid 601 through 730 )
46X-RAY DIFFRACTION46chain 'I' and (resid 731 through 875 )
47X-RAY DIFFRACTION47chain 'I' and (resid 876 through 923 )
48X-RAY DIFFRACTION48chain 'J' and (resid 5 through 14 )
49X-RAY DIFFRACTION49chain 'J' and (resid 15 through 29 )
50X-RAY DIFFRACTION50chain 'J' and (resid 30 through 39 )
51X-RAY DIFFRACTION51chain 'J' and (resid 40 through 70 )
52X-RAY DIFFRACTION52chain 'J' and (resid 71 through 84 )
53X-RAY DIFFRACTION53chain 'J' and (resid 85 through 103 )
54X-RAY DIFFRACTION54chain 'K' and (resid 3 through 12 )
55X-RAY DIFFRACTION55chain 'K' and (resid 13 through 24 )
56X-RAY DIFFRACTION56chain 'K' and (resid 25 through 53 )
57X-RAY DIFFRACTION57chain 'L' and (resid 18 through 43 )
58X-RAY DIFFRACTION58chain 'L' and (resid 44 through 68 )

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月:コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか? / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例:em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る