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- PDB-7d8g: The crystal structure of nucleotide phosphatase Sa1684 from Staph... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7d8g | ||||||
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Title | The crystal structure of nucleotide phosphatase Sa1684 from Staphylococcus aureus | ||||||
![]() | UPF0374 protein SA1684 | ||||||
![]() | CYTOSOLIC PROTEIN / nucleotide phospatase | ||||||
Function / homology | ![]() nucleoside diphosphate phosphatase / nucleoside diphosphate phosphatase activity / ribonucleoside triphosphate phosphatase activity / nucleoside-triphosphate phosphatase / magnesium ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wang, Z. / Li, X. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The structural mechanism for the nucleoside tri- and diphosphate hydrolysis activity of Ntdp from Staphylococcus aureus. Authors: Wang, Z. / Shen, H. / He, B. / Teng, M. / Guo, Q. / Li, X. #1: ![]() Title: Novel Nucleoside Diphosphatase Contributes to Staphylococcus aureus Virulence Authors: Kenta, I. / Yuki, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.6 KB | Display | ![]() |
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PDB format | ![]() | 37.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 9.8 KB | Display | |
Data in CIF | ![]() | 13.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 21734.592 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: N315 / Gene: SA1684 / Production host: ![]() ![]() | ||||||
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#2: Chemical | ChemComp-CIT / | ||||||
#3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.08 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion, hanging drop / Details: 20% PEG400, 0.1M sodium citrate pH 5.5. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jan 18, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98752 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. obs: 29587 / % possible obs: 98.6 % / Redundancy: 6.7 % / Rmerge(I) obs: 0.051 / Net I/σ(I): 27.25 |
Reflection shell | Resolution: 1.5→1.53 Å / Num. unique obs: 1466 / CC1/2: 0.968 / % possible all: 95.3 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 52.17 Å2 / Biso mean: 14.504 Å2 / Biso min: 5.62 Å2
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Refinement step | Cycle: final / Resolution: 1.5→30.32 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.501→1.54 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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