Mass: 22.990 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: Na
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-1H NOESY
1
2
1
isotropic
1
2D 1H-1H TOCSY
1
3
1
isotropic
1
2D 1H-1H COSY
-
Sample preparation
Details
Type: solution Contents: 0.4 mM No RNA, 0.4 mM No Ligand, 10.0 mM No NaPi, 0.02 mM No DSS, 0.1 mM No EDTA, 100% D2O Details: 0.4 mM RNA, 0.4 mM ligand DB213, 10 mM NaPi (pH 7), 0.02 mM DSS, 0.1 mM EDTA. Label: No label / Solvent system: 100% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.4mM
RNA
No
1
0.4mM
Ligand
No
1
10.0mM
NaPi
No
1
0.02mM
DSS
No
1
0.1mM
EDTA
No
1
Sample conditions
Ionic strength: 10.0 mM / Ionic strength err: 0.1 / Label: Condition_1 / pH: 7.0 / PH err: 0.1 / Pressure: 1 bar / Pressure err: 0.01 / Temperature: 283 K / Temperature err: 0.2
-
NMR measurement
NMR spectrometer
Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz
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