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Open data
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Basic information
Entry | Database: PDB / ID: 7d0e | |||||||||
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Title | Crystal structure of FIP200 Claw/p-CCPG1 FIR2 | |||||||||
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![]() | SIGNALING PROTEIN/PROTEIN BINDING / autophagy / FIP200 / CCPG1 / SIGNALING PROTEIN / SIGNALING PROTEIN-PROTEIN BINDING complex | |||||||||
Function / homology | ![]() regulation of Rho guanyl-nucleotide exchange factor activity / regulation of protein lipidation / ribophagy / glycophagy / Atg1/ULK1 kinase complex / phagophore assembly site membrane / piecemeal microautophagy of the nucleus / phagophore assembly site / reticulophagy / Macroautophagy ...regulation of Rho guanyl-nucleotide exchange factor activity / regulation of protein lipidation / ribophagy / glycophagy / Atg1/ULK1 kinase complex / phagophore assembly site membrane / piecemeal microautophagy of the nucleus / phagophore assembly site / reticulophagy / Macroautophagy / autophagosome membrane / positive regulation of cell size / autophagosome assembly / positive regulation of autophagy / positive regulation of cell cycle / extrinsic apoptotic signaling pathway / protein-membrane adaptor activity / liver development / negative regulation of extrinsic apoptotic signaling pathway / positive regulation of JNK cascade / autophagy / heart development / nuclear membrane / membrane => GO:0016020 / molecular adaptor activity / lysosome / positive regulation of protein phosphorylation / cell cycle / negative regulation of cell population proliferation / positive regulation of cell population proliferation / endoplasmic reticulum membrane / protein kinase binding / positive regulation of transcription by RNA polymerase II / membrane / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Zhou, Z.X. / Pan, L.F. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Phosphorylation regulates the binding of autophagy receptors to FIP200 Claw domain for selective autophagy initiation. Authors: Zhou, Z. / Liu, J. / Fu, T. / Wu, P. / Peng, C. / Gong, X. / Wang, Y. / Zhang, M. / Li, Y. / Wang, Y. / Xu, X. / Li, M. / Pan, L. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70.1 KB | Display | ![]() |
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PDB format | ![]() | 47 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 847.9 KB | Display | ![]() |
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Full document | ![]() | 849.1 KB | Display | |
Data in XML | ![]() | 8.5 KB | Display | |
Data in CIF | ![]() | 11.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7czgC ![]() 7czmC ![]() 6dceS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 12039.970 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1496.487 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-GO9 / |
#4: Chemical | ChemComp-PEG / |
#5: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.57 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / Details: KCl, Pentaerythritol ethoxylate (15/4 EO/OH), MES |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Apr 12, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97876 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→50 Å / Num. obs: 23548 / % possible obs: 99.8 % / Redundancy: 25.1 % / Biso Wilson estimate: 12.28 Å2 / Rmerge(I) obs: 0.037 / Net I/σ(I): 98.9 |
Reflection shell | Resolution: 1.4→1.42 Å / Num. unique obs: 1143 / Rpim(I) all: 0.174 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6DCE Resolution: 1.4→25.07 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 24.49 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→25.07 Å
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Refine LS restraints |
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