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Open data
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Basic information
Entry | Database: PDB / ID: 7csg | ||||||
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Title | AtPrR2 in apo form | ||||||
![]() | Pinoresinol reductase 2 | ||||||
![]() | OXIDOREDUCTASE / AtPrR2 / homodimer / PLANT PROTEIN | ||||||
Function / homology | (-)-pinoresinol reductase / lignan biosynthetic process / pinoresinol reductase activity / Phenylcoumaran benzylic ether reductase-like / NmrA-like domain / NmrA-like family / plasmodesma / NAD(P)-binding domain superfamily / Pinoresinol reductase 2![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shao, K. / Zhang, P. | ||||||
![]() | ![]() Title: Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases. Authors: Xiao, Y. / Shao, K. / Zhou, J. / Wang, L. / Ma, X. / Wu, D. / Yang, Y. / Chen, J. / Feng, J. / Qiu, S. / Lv, Z. / Zhang, L. / Zhang, P. / Chen, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 160.4 KB | Display | ![]() |
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PDB format | ![]() | 101.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 440.5 KB | Display | ![]() |
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Full document | ![]() | 443.3 KB | Display | |
Data in XML | ![]() | 24.8 KB | Display | |
Data in CIF | ![]() | 36.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7cs2C ![]() 7cs3C ![]() 7cs4C ![]() 7cs5C ![]() 7cs6C ![]() 7cs7C ![]() 7cs8C ![]() 7cs9C ![]() 7csaC ![]() 7csbC ![]() 7cscC ![]() 7csdC ![]() 7cseC ![]() 7csfC ![]() 7cshC ![]() 1qydS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35462.414 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.08 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.2M magnesium chloride, 0.1 M sodium HEPES, pH 7.5, 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR555 FLAT PANEL / Detector: IMAGE PLATE / Date: Mar 12, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 1.996→34.162 Å / Num. obs: 39962 / % possible obs: 97.6 % / Redundancy: 3.6 % / Biso Wilson estimate: 22.5003133031 Å2 / Rmerge(I) obs: 0.115 / Net I/σ(I): 24.3 |
Reflection shell | Resolution: 2→2.07 Å / Rmerge(I) obs: 0.422 / Num. unique obs: 3909 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1QYD Resolution: 1.99689386689→34.1611618469 Å / SU ML: 0.217670236234 / Cross valid method: FREE R-VALUE / σ(F): 1.98030952507 / Phase error: 24.6104888862 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.1118130414 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.99689386689→34.1611618469 Å
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Refine LS restraints |
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LS refinement shell |
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