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Open data
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Basic information
Entry | Database: PDB / ID: 7cqm | ||||||
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Title | PlsY grown in LCP soaked with selenourea for 22 min | ||||||
![]() | Glycerol-3-phosphate acyltransferase | ||||||
![]() | TRANSFERASE / selenourea / Se-SAD / LCP / PlsY | ||||||
Function / homology | ![]() acyl phosphate:glycerol-3-phosphate acyltransferase / acyl-phosphate glycerol-3-phosphate acyltransferase activity / phospholipid biosynthetic process / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Luo, Z.P. / Li, D.F. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Selenourea for experimental phasing of membrane protein crystals grown in lipid cubic phase Authors: Luo, Z.P. / Gu, W. / Wang, Y. / Tang, Y. / Li, D.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 106 KB | Display | ![]() |
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PDB format | ![]() | 82.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 5.7 MB | Display | ![]() |
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Full document | ![]() | 5.8 MB | Display | |
Data in XML | ![]() | 21.2 KB | Display | |
Data in CIF | ![]() | 28.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21863.992 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: O66905, acyl phosphate:glycerol-3-phosphate acyltransferase #2: Chemical | ChemComp-SEY / #3: Chemical | ChemComp-SO4 / #4: Chemical | ChemComp-79M / ( #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.95 Å3/Da / Density % sol: 58.27 % |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase Details: 25-35% (v/v) triethylene glycol, 0.1 M ammonium sulfate, 0.1 M glycine |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 31, 2019 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 0.97907 Å / Relative weight: 1 | |||||||||||||||
Reflection twin |
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Reflection | Resolution: 1.8→50 Å / Num. obs: 46884 / % possible obs: 99.6 % / Redundancy: 6.7 % / CC1/2: 1 / Rmerge(I) obs: 0.094 / Rpim(I) all: 0.039 / Net I/σ(I): 1.69 | |||||||||||||||
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.973 / Mean I/σ(I) obs: 18.59 / Num. unique obs: 4730 / CC1/2: 0.687 / Rpim(I) all: 0.4 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 108.92 Å2 / Biso mean: 25.337 Å2 / Biso min: 9.09 Å2
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Refinement step | Cycle: final / Resolution: 1.8→36.37 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.845 Å
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