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Open data
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Basic information
Entry | Database: PDB / ID: 7chs | ||||||
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Title | Crystal structure of SARS-CoV-2 antibody P22A-1D1 with RBD | ||||||
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![]() | VIRAL PROTEIN / spike / receptor binding domain / antibody | ||||||
Function / homology | ![]() Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / entry receptor-mediated virion attachment to host cell / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wang, X. / Zhang, L. / Ge, J. / Wang, R. / Zhang, Q. | ||||||
![]() | ![]() Title: Potent and protective IGHV3-53/3-66 public antibodies and their shared escape mutant on the spike of SARS-CoV-2. Authors: Zhang, Q. / Ju, B. / Ge, J. / Chan, J.F. / Cheng, L. / Wang, R. / Huang, W. / Fang, M. / Chen, P. / Zhou, B. / Song, S. / Shan, S. / Yan, B. / Zhang, S. / Ge, X. / Yu, J. / Zhao, J. / Wang, ...Authors: Zhang, Q. / Ju, B. / Ge, J. / Chan, J.F. / Cheng, L. / Wang, R. / Huang, W. / Fang, M. / Chen, P. / Zhou, B. / Song, S. / Shan, S. / Yan, B. / Zhang, S. / Ge, X. / Yu, J. / Zhao, J. / Wang, H. / Liu, L. / Lv, Q. / Fu, L. / Shi, X. / Yuen, K.Y. / Liu, L. / Wang, Y. / Chen, Z. / Zhang, L. / Wang, X. / Zhang, Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 140.7 KB | Display | ![]() |
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PDB format | ![]() | 105.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.3 KB | Display | ![]() |
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Full document | ![]() | 467.4 KB | Display | |
Data in XML | ![]() | 25.8 KB | Display | |
Data in CIF | ![]() | 37.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7choC ![]() 7chpC ![]() 6m0jS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 24600.631 Da / Num. of mol.: 1 / Fragment: receptor binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: S, 2 / Production host: ![]() ![]() |
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#2: Antibody | Mass: 23007.646 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Antibody | Mass: 23262.945 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#4: Sugar | ChemComp-NAG / |
#5: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.34 Å3/Da / Density % sol: 63.2 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 0.1M potassium chloride, 0.1M NaHEPES, pH 7.0, 15% PEG 5000MME |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 17, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9769 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 36392 / % possible obs: 100 % / Redundancy: 6.7 % / CC1/2: 0.989 / Rmerge(I) obs: 0.114 / Net I/σ(I): 13.1 |
Reflection shell | Resolution: 2.4→2.46 Å / Rmerge(I) obs: 0.983 / Num. unique obs: 3573 / CC1/2: 0.655 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6M0J Resolution: 2.401→47.706 Å / SU ML: 0.27 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 23.05 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 124.98 Å2 / Biso mean: 42.0731 Å2 / Biso min: 21.83 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.401→47.706 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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