+Open data
-Basic information
Entry | Database: PDB / ID: 7chq | ||||||
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Title | AcrIE2 | ||||||
Components | anti-CRISPR AcrIE2 | ||||||
Keywords | IMMUNE SYSTEM / AcrIE2 / CRISPR Cascade inhibitor. | ||||||
Function / homology | Uncharacterized protein Function and homology information | ||||||
Biological species | Pseudomonas phage JBD16C (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / AB INITIO PHASING / Resolution: 1.33 Å | ||||||
Authors | Lee, S.Y. / Park, H.H. | ||||||
Funding support | Korea, Republic Of, 1items
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Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2020 Title: A 1.3 angstrom high-resolution crystal structure of an anti-CRISPR protein, AcrI E2. Authors: Lee, S.Y. / Kim, G.E. / Kim, Y.G. / Park, H.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7chq.cif.gz | 30.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7chq.ent.gz | 19 KB | Display | PDB format |
PDBx/mmJSON format | 7chq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7chq_validation.pdf.gz | 416.1 KB | Display | wwPDB validaton report |
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Full document | 7chq_full_validation.pdf.gz | 416.1 KB | Display | |
Data in XML | 7chq_validation.xml.gz | 6 KB | Display | |
Data in CIF | 7chq_validation.cif.gz | 7.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ch/7chq ftp://data.pdbj.org/pub/pdb/validation_reports/ch/7chq | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10432.380 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas phage JBD16C (virus) / Gene: JBD16C_30 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A125RN64 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.88 Å3/Da / Density % sol: 28.48 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop Details: 30% PEG 8000, 0.2M lithium sulfate, 0.1M sodium acetate pH 4.8 |
-Data collection
Diffraction | Mean temperature: 125 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 5C (4A) / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: May 26, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.21→28.05 Å / Num. obs: 275146 / % possible obs: 95.49 % / Redundancy: 12.7 % / Rrim(I) all: 0.047 / Net I/σ(I): 31.1 |
Reflection shell | Resolution: 1.24→1.27 Å / Num. unique obs: 216131 / Rrim(I) all: 0.369 |
-Processing
Software |
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 1.33→28.05 Å / SU ML: 0.14 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 22.36 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.33→28.05 Å
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Refine LS restraints |
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LS refinement shell |
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