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Open data
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Basic information
Entry | Database: PDB / ID: 7bwf | ||||||||||||
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Title | YoeB-YefM complex from Staphylococcus aureus | ||||||||||||
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![]() | TOXIN/ANTITOXIN / Toxin-Antitoxin complex ribosome-dependent ribonuclease ribosome-independent ribonuclease Toxin inhibitor / TOXIN / TOXIN-ANTITOXIN complex | ||||||||||||
Function / homology | ![]() | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Lee, B.-J. / Eun, H.-J. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of the YoeBSa1-YefMSa1 complex from Staphylococcus aureus. Authors: Eun, H.-J. / Lee, K.-Y. / Kim, D.-G. / Im, D.S. / Lee, B.-J. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 223.8 KB | Display | ![]() |
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PDB format | ![]() | 183.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.6 KB | Display | ![]() |
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Full document | ![]() | 448.6 KB | Display | |
Data in XML | ![]() | 16.3 KB | Display | |
Data in CIF | ![]() | 22.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2a6qS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10325.804 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | | Mass: 10335.587 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: yefM, BTN44_14530, EP54_09735, EQ90_00255, HMPREF3211_00052, M1K003_0949, NCTC10654_02579, NCTC10702_03766, NCTC5664_01444, NCTC6133_03236, NCTC7988_02488, RK64_12840, SAKG03_24010 Production host: ![]() ![]() #3: Protein | | Mass: 10248.509 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: yefM, BTN44_14530, EP54_09735, EQ90_00255, HMPREF3211_00052, M1K003_0949, NCTC10654_02579, NCTC10702_03766, NCTC5664_01444, NCTC6133_03236, NCTC7988_02488, RK64_12840, SAKG03_24010 Production host: ![]() ![]() #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 44.97 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 0.08 M sodium chloride, 0.02 M magnesium chloride hexahydrate, 0.04 M sodium cacodylate trihydrate pH 7.0, 40% v/v (+/-)-2-methyl-2,4-pentanediol, and 0.012 M spermine tetrahydrochloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jul 31, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97942 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→50 Å / Num. obs: 38302 / % possible obs: 97.4 % / Redundancy: 3.4 % / Rmerge(I) obs: 0.069 / Net I/σ(I): 26.9 |
Reflection shell | Resolution: 1.7→1.73 Å / Rmerge(I) obs: 0.611 / Mean I/σ(I) obs: 2.3 / Num. unique obs: 1944 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2A6Q Resolution: 1.7→44.832 Å / SU ML: 0.21 / Cross valid method: THROUGHOUT / σ(F): 2 / Phase error: 26.31
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 124.07 Å2 / Biso mean: 43.0848 Å2 / Biso min: 18.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.7→44.832 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Origin x: 17.3212 Å / Origin y: 9.135 Å / Origin z: 10.9917 Å
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Refinement TLS group |
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