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Yorodumi- PDB-7bm6: Structure-function analysis of a new PL17 oligoalginate lyase fro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7bm6 | |||||||||
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Title | Structure-function analysis of a new PL17 oligoalginate lyase from the marine bacterium Zobellia galactanivorans DsijT | |||||||||
Components | Alginate lyase, family PL17 | |||||||||
Keywords | HYDROLASE / alginate lyase / delta-guluronate complex / family PL17 / exo-acting marine enzyme | |||||||||
Function / homology | mannuronate-specific alginate lyase / poly(beta-D-mannuronate) lyase activity / Heparinase II/III-like / Heparinase II/III-like protein / Alginate lyase domain / Alginate lyase / Chondroitin AC/alginate lyase / periplasmic space / Alginate lyase, family PL17 Function and homology information | |||||||||
Biological species | Zobellia galactanivorans (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.16 Å | |||||||||
Authors | Czjzek, M. / Roret, T. / Jouanneau, D. / Le Duff, N. / Jeudy, A. | |||||||||
Funding support | France, European Union, 2items
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Citation | Journal: Glycobiology / Year: 2021 Title: Structure-function analysis of a new PL17 oligoalginate lyase from the marine bacterium Zobellia galactanivorans DsijT. Authors: Jouanneau, D. / Klau, L.J. / Larocque, R. / Jaffrennou, A. / Duval, G. / Le Duff, N. / Roret, T. / Jeudy, A. / Aachmann, F.L. / Czjzek, M. / Thomas, F. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7bm6.cif.gz | 585.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7bm6.ent.gz | 480.9 KB | Display | PDB format |
PDBx/mmJSON format | 7bm6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bm/7bm6 ftp://data.pdbj.org/pub/pdb/validation_reports/bm/7bm6 | HTTPS FTP |
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-Related structure data
Related structure data | 7bjtSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein / Sugars , 2 types, 4 molecules AB
#1: Protein | Mass: 85059.336 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The active site tyrosine Y274 was mutated to alanine Y247A The N-terminal peptide (1-23) was not included in recombinant construction defined by electron density Source: (gene. exp.) Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij) (bacteria) Strain: DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij Gene: alyA3, zobellia_2624 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: G0LCA3, mannuronate-specific alginate lyase #2: Polysaccharide | Source method: isolated from a genetically manipulated source |
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-Non-polymers , 4 types, 919 molecules
#3: Chemical | #4: Chemical | ChemComp-MG / #5: Chemical | ChemComp-GOL / | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.84 Å3/Da / Density % sol: 68 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 7 Details: protein solution at 13.4 mg.ml-1, supplemented with 1.6 mM of oligo-mannuronate of DP3, was mixed with 0.2 micro-l of crystallization solution that contained 2.4 M sodium malonate (dibasic ...Details: protein solution at 13.4 mg.ml-1, supplemented with 1.6 mM of oligo-mannuronate of DP3, was mixed with 0.2 micro-l of crystallization solution that contained 2.4 M sodium malonate (dibasic monohydrate) and equilibrated against a reservoir containing 100 micro-l. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.98 Å |
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Jan 25, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.16→45 Å / Num. obs: 137171 / % possible obs: 99.6 % / Redundancy: 21.16 % / CC1/2: 0.999 / Rpim(I) all: 0.054 / Rsym value: 0.12 / Net I/σ(I): 16.3 |
Reflection shell | Resolution: 2.16→2.21 Å / Redundancy: 18 % / Rmerge(I) obs: 1.57 / Num. unique obs: 7343 / CC1/2: 0.641 / Rpim(I) all: 0.356 / % possible all: 99.2 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7BJT Resolution: 2.16→45 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.95 / SU B: 6.495 / SU ML: 0.09 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.134 / ESU R Free: 0.125 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 108.27 Å2 / Biso mean: 33.489 Å2 / Biso min: 17.67 Å2
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Refinement step | Cycle: final / Resolution: 2.16→45 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.16→2.216 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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