+Open data
-Basic information
Entry | Database: PDB / ID: 7bk5 | ||||||
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Title | PfCopC mutant - E27A | ||||||
Components | Putative copper resistance protein | ||||||
Keywords | METAL BINDING PROTEIN / Copper binding / copper transport | ||||||
Function / homology | Function and homology information copper ion transport / response to copper ion / periplasmic space / copper ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Pseudomonas fluorescens (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.54 Å | ||||||
Authors | Muderspach, S.J. / Ipsen, J. / Rollan, C.H. / Bertelsen, A.B. / Norholm, M.H.H. / Johansen, K.S. / Lo Leggio, L. | ||||||
Funding support | Denmark, 1items
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Citation | Journal: Febs Lett. / Year: 2021 Title: Copper binding and reactivity at the histidine brace motif: insights from mutational analysis of the Pseudomonas fluorescens copper chaperone CopC. Authors: Ipsen, J.O. / Hernandez-Rollan, C. / Muderspach, S.J. / Brander, S. / Bertelsen, A.B. / Jensen, P.E. / Norholm, M.H.H. / Lo Leggio, L. / Johansen, K.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7bk5.cif.gz | 37.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7bk5.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 7bk5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7bk5_validation.pdf.gz | 920.3 KB | Display | wwPDB validaton report |
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Full document | 7bk5_full_validation.pdf.gz | 920.8 KB | Display | |
Data in XML | 7bk5_validation.xml.gz | 7.3 KB | Display | |
Data in CIF | 7bk5_validation.cif.gz | 9.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bk/7bk5 ftp://data.pdbj.org/pub/pdb/validation_reports/bk/7bk5 | HTTPS FTP |
-Related structure data
Related structure data | 7bk6C 7bk7C 6nfqS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 10052.258 Da / Num. of mol.: 1 / Mutation: E27A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas fluorescens (strain SBW25) (bacteria) Strain: SBW25 / Gene: PFLU_3946 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: C3JYL7 | ||||||
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#2: Chemical | ChemComp-CU / | ||||||
#3: Chemical | ChemComp-CL / #4: Chemical | ChemComp-PO4 / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.87 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 2.52 M ammonium phosphate dibasic, 0.09 M Tris, 3% (w/v) D-sorbitol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.87313 Å |
Detector | Type: DECTRIS PILATUS3 X 2M / Detector: PIXEL / Date: Oct 7, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87313 Å / Relative weight: 1 |
Reflection | Resolution: 1.54→50 Å / Num. obs: 13059 / % possible obs: 99.9 % / Redundancy: 5.47 % / CC1/2: 0.998 / Rrim(I) all: 0.01011 / Net I/σ(I): 10.11 |
Reflection shell | Resolution: 1.54→1.58 Å / Num. unique obs: 1822 / CC1/2: 0.525 / Rrim(I) all: 0.1753 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6NFQ Resolution: 1.54→40.975 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.963 / SU B: 2.781 / SU ML: 0.095 / Cross valid method: FREE R-VALUE / ESU R: 0.1 / ESU R Free: 0.096 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.664 Å2
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Refinement step | Cycle: LAST / Resolution: 1.54→40.975 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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