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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7bgw | |||||||||
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タイトル | 14-3-3 sigma with Pin1 binding site pS72 and covalently bound LvD1011 | |||||||||
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![]() | PEPTIDE BINDING PROTEIN / 1433 / Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 | |||||||||
機能・相同性 | ![]() cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / postsynaptic cytosol / mitogen-activated protein kinase kinase binding / regulation of mitotic nuclear division / negative regulation of SMAD protein signal transduction ...cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / postsynaptic cytosol / mitogen-activated protein kinase kinase binding / regulation of mitotic nuclear division / negative regulation of SMAD protein signal transduction / regulation of epidermal cell division / protein kinase C inhibitor activity / positive regulation of epidermal cell differentiation / keratinocyte development / keratinization / PI5P Regulates TP53 Acetylation / negative regulation of amyloid-beta formation / cytoskeletal motor activity / Regulation of localization of FOXO transcription factors / keratinocyte proliferation / phosphoserine residue binding / RHO GTPases Activate NADPH Oxidases / Activation of BAD and translocation to mitochondria / negative regulation of keratinocyte proliferation / establishment of skin barrier / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / protein peptidyl-prolyl isomerization / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / negative regulation of stem cell proliferation / positive regulation of protein dephosphorylation / RHO GTPases activate PKNs / protein kinase A signaling / protein sequestering activity / negative regulation of innate immune response / protein export from nucleus / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / positive regulation of protein export from nucleus / release of cytochrome c from mitochondria / ciliary basal body / positive regulation of GTPase activity / regulation of cytokinesis / negative regulation of protein binding / stem cell proliferation / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / Translocation of SLC2A4 (GLUT4) to the plasma membrane / Negative regulators of DDX58/IFIH1 signaling / TP53 Regulates Metabolic Genes / phosphoprotein binding / synapse organization / negative regulation of transforming growth factor beta receptor signaling pathway / regulation of protein phosphorylation / negative regulation of protein kinase activity / regulation of protein stability / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / tau protein binding / neuron differentiation / negative regulation of protein catabolic process / negative regulation of ERK1 and ERK2 cascade / ISG15 antiviral mechanism / beta-catenin binding / intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of canonical Wnt signaling pathway / positive regulation of protein binding / midbody / positive regulation of cell growth / regulation of gene expression / Regulation of TP53 Activity through Phosphorylation / protein stabilization / response to hypoxia / regulation of cell cycle / nuclear speck / cadherin binding / positive regulation of protein phosphorylation / cell cycle / glutamatergic synapse / protein kinase binding / negative regulation of transcription by RNA polymerase II / signal transduction / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular exosome / nucleoplasm / identical protein binding / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | ![]() ![]() | |||||||||
![]() | Wolter, M. / Dijck, L.v. / Cossar, P.J. / Ottmann, C. | |||||||||
資金援助 | 2件
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![]() | ![]() タイトル: Reversible Covalent Imine-Tethering for Selective Stabilization of 14-3-3 Hub Protein Interactions. 著者: Cossar, P.J. / Wolter, M. / van Dijck, L. / Valenti, D. / Levy, L.M. / Ottmann, C. / Brunsveld, L. | |||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 73.7 KB | 表示 | ![]() |
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PDB形式 | ![]() | 51.3 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 633 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 633 KB | 表示 | |
XML形式データ | ![]() | 14.4 KB | 表示 | |
CIF形式データ | ![]() | 22.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7aogSC ![]() 7axnC ![]() 7ayfC ![]() 7az1C ![]() 7az2C ![]() 7bdpC ![]() 7bdtC ![]() 7bdyC ![]() 7bfwC ![]() 7bg3C ![]() 7bgqC ![]() 7bgrC ![]() 7bgvC ![]() 7nifC ![]() 7nigC ![]() 7nixC ![]() 7nj6C ![]() 7nj8C ![]() 7njaC ![]() 7nqpC ![]() 7nrkC ![]() 7nrlC ![]() 7nsvC S: 精密化の開始モデル C: 同じ文献を引用 ( |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
-タンパク質 / タンパク質・ペプチド , 2種, 2分子 AP
#1: タンパク質 | 分子量: 28226.518 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: タンパク質・ペプチド | 分子量: 2195.378 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) ![]() |
-非ポリマー , 5種, 352分子 ![](data/chem/img/TLQ.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
#3: 化合物 | ChemComp-TLQ / | ||||||
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#4: 化合物 | #5: 化合物 | ChemComp-CL / | #6: 化合物 | ChemComp-NA / | #7: 水 | ChemComp-HOH / | |
-詳細
研究の焦点であるリガンドがあるか | Y |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.37 Å3/Da / 溶媒含有率: 48.09 % |
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結晶化 | 温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: 0.095 M HEPES Na pH 7.1, 27% PEG400, 0.19M Calcium chloride, 5% Glycerol |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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放射光源 | 由来: SEALED TUBE / タイプ: RIGAKU MICROMAX-003 / 波長: 1.54187 Å | ||||||||||||||||||||||||||||||
検出器 | タイプ: DECTRIS PILATUS 200K / 検出器: PIXEL / 日付: 2020年1月29日 | ||||||||||||||||||||||||||||||
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | ||||||||||||||||||||||||||||||
放射波長 | 波長: 1.54187 Å / 相対比: 1 | ||||||||||||||||||||||||||||||
反射 | 解像度: 1.9→34.424 Å / Num. obs: 22305 / % possible obs: 96.4 % / 冗長度: 6.5 % / Biso Wilson estimate: 10.79 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.094 / Rpim(I) all: 0.039 / Rrim(I) all: 0.102 / Net I/σ(I): 13.3 | ||||||||||||||||||||||||||||||
反射 シェル | Diffraction-ID: 1
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 7AOG 解像度: 1.9→34.424 Å / SU ML: 0.16 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 20.1 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso max: 70.45 Å2 / Biso mean: 14.3277 Å2 / Biso min: 2.45 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: final / 解像度: 1.9→34.424 Å
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拘束条件 |
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LS精密化 シェル | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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