A: DtxR family iron (Metal) dependent repressor B: DtxR family iron (Metal) dependent repressor C: DtxR family iron (Metal) dependent repressor D: DtxR family iron (Metal) dependent repressor E: consensus DNA-binding sequence F: consensus DNA-binding sequence aa: DtxR family iron (Metal) dependent repressor dd: DtxR family iron (Metal) dependent repressor ヘテロ分子
分子量: 25587.316 Da / 分子数: 6 / 由来タイプ: 組換発現 詳細: There is a domain swap with symmetry mates. The C-terminal domains associated with chains A and D originate from chains D and A of a symmetry mate, respectively, and have therefore been named ...詳細: There is a domain swap with symmetry mates. The C-terminal domains associated with chains A and D originate from chains D and A of a symmetry mate, respectively, and have therefore been named chains dd and aa. The swap is only possible for chains A and D, but not for chains B and C, due to crystal packing interactions. 由来: (組換発現) Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) (バクテリア) 株: ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338 遺伝子: A8924_2181 / プラスミド: pET-28a(+) / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: A0A2A9J1W2
There is a domain swap with symmetry mates. The C-terminal domains associated with chains A and D ...There is a domain swap with symmetry mates. The C-terminal domains associated with chains A and D originate from chains D and A of a symmetry mate, respectively, and have therefore been named chains dd and aa. The swap is only possible for chains A and D, but not for chains B and C, due to crystal packing interactions.
研究の焦点であるリガンドがあるか
Y
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.44 Å3/Da / 溶媒含有率: 64.22 %
結晶化
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6.5 詳細: 29%(w/v) PEG 3350, 0.28 M ammonium sulfate, 0.1 M MES
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データ収集
回折
平均測定温度: 100 K / Serial crystal experiment: N
放射光源
由来: シンクロトロン / サイト: MAX IV / ビームライン: BioMAX / 波長: 0.97622 Å
解像度: 2.34→93.16 Å / Cor.coef. Fo:Fc: 0.888 / Cor.coef. Fo:Fc free: 0.88 / SU B: 11.716 / SU ML: 0.264 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 1.576 / ESU R Free: 0.402 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2831
1869
5.1 %
RANDOM
Rwork
0.2673
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obs
0.268
35059
53.86 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK