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Yorodumi- PDB-2wsu: Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre -
+Open data
-Basic information
Entry | Database: PDB / ID: 2wsu | ||||||
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Title | Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre | ||||||
Components | PUTATIVE FIBER PROTEIN | ||||||
Keywords | VIRAL PROTEIN / CARBOHYDRATE RECOGNITION DOMAIN / TANDEM-REPEAT-TYPE | ||||||
Function / homology | Function and homology information galactoside binding / viral capsid / carbohydrate binding / cell adhesion / membrane raft / virion attachment to host cell / plasma membrane Similarity search - Function | ||||||
Biological species | PORCINE ADENOVIRUS 4 | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Guardado-Calvo, P. / Munoz, E.M. / Llamas-Saiz, A.L. / Fox, G.C. / Glasgow, J.N. / van Raaij, M.J. | ||||||
Citation | Journal: J. Virol. / Year: 2010 Title: Crystallographic structure of porcine adenovirus type 4 fiber head and galectin domains. Authors: Guardado-Calvo, P. / Munoz, E.M. / Llamas-Saiz, A.L. / Fox, G.C. / Kahn, R. / Curiel, D.T. / Glasgow, J.N. / van Raaij, M.J. #1: Journal: Virus Res. / Year: 1995 Title: Sequence Analysis of the Fiber Genomic Region of a Porcine Adenovirus Predicts a Novel Fiber Protein. Authors: Kleiboeker, S.B. #2: Journal: Cancer Biol.Ther. / Year: 2008 Title: Characterization of Infectivity of Knob-Modified Adenoviral Vectors in Glioma. Authors: Paul, C.P.L. / Everts, M. / Glasgow, J.N. / Dent, P. / Fisher, P.B. / Ulasov, I.V. / Lesniak, M.S. / Stoff-Khalili, M.A. / Roth, J.C. / Preuss, M.A. / Dirven, C.M.F. / Lamfers, M.L.M. / ...Authors: Paul, C.P.L. / Everts, M. / Glasgow, J.N. / Dent, P. / Fisher, P.B. / Ulasov, I.V. / Lesniak, M.S. / Stoff-Khalili, M.A. / Roth, J.C. / Preuss, M.A. / Dirven, C.M.F. / Lamfers, M.L.M. / Siegal, G.P. / Zhu, Z.B. / Curiel, D.T. #3: Journal: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. Year: 2009 Title: Crystallization of the head and galectin-like domains of porcine adenovirus isolate NADC-1 fibre. Authors: Guardado-Calvo, P. / Llamas-Saiz, A.L. / Fox, G.C. / Glasgow, J.N. / van Raaij, M.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2wsu.cif.gz | 520.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2wsu.ent.gz | 433.1 KB | Display | PDB format |
PDBx/mmJSON format | 2wsu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2wsu_validation.pdf.gz | 507.6 KB | Display | wwPDB validaton report |
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Full document | 2wsu_full_validation.pdf.gz | 521.6 KB | Display | |
Data in XML | 2wsu_validation.xml.gz | 51 KB | Display | |
Data in CIF | 2wsu_validation.cif.gz | 71.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ws/2wsu ftp://data.pdbj.org/pub/pdb/validation_reports/ws/2wsu | HTTPS FTP |
-Related structure data
Related structure data | 2wstC 2wsvC 2wt0C 2wt1C 2wt2C 1a3kS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 37909.156 Da / Num. of mol.: 4 / Fragment: GALECTIN DOMAIN, RESIDUES 393-703 Source method: isolated from a genetically manipulated source Source: (gene. exp.) PORCINE ADENOVIRUS 4 / Variant: NADC-1 ISOLATE / Plasmid: PET28C / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q83467 #2: Chemical | ChemComp-GOL / #3: Chemical | ChemComp-NO3 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 37.6 % / Description: NONE |
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Crystal grow | pH: 8 Details: 10 MM TRIS-HCL PH 8.0, 1 MM EDTA, 25% (W/V) POLY-ETHYLENE GLYCOL 3350, 500 MM SODIUM NITRATE, 2 MM SIALIC ACID |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-2 / Wavelength: 0.939 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jul 20, 2008 / Details: MIRRORS |
Radiation | Monochromator: DIAMOND (111), GE(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.939 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→40 Å / Num. obs: 89027 / % possible obs: 96.1 % / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 21.6 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 10 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.35 / Mean I/σ(I) obs: 3.7 / % possible all: 90.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1A3K Resolution: 1.9→35 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.951 / SU B: 10.559 / SU ML: 0.134 / Cross valid method: THROUGHOUT / ESU R: 0.191 / ESU R Free: 0.174 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.322 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→35 Å
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Refine LS restraints |
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