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- PDB-7auw: Inhibitory complex of human meprin beta with mouse fetuin-B. -

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Basic information

Entry
Database: PDB / ID: 7auw
TitleInhibitory complex of human meprin beta with mouse fetuin-B.
Components
  • Fetuin-B
  • Meprin A subunit beta
KeywordsHYDROLASE INHIBITOR / protease inhibitor complex / zinc metallopeptidase / cystatin-type modules / HYDROLASE
Function / homology
Function and homology information


meprin B / meprin A complex / metalloendopeptidase inhibitor activity / binding of sperm to zona pellucida / negative regulation of endopeptidase activity / cysteine-type endopeptidase inhibitor activity / single fertilization / metalloendopeptidase activity / inflammatory response / proteolysis ...meprin B / meprin A complex / metalloendopeptidase inhibitor activity / binding of sperm to zona pellucida / negative regulation of endopeptidase activity / cysteine-type endopeptidase inhibitor activity / single fertilization / metalloendopeptidase activity / inflammatory response / proteolysis / extracellular space / zinc ion binding / extracellular region / identical protein binding / plasma membrane
Similarity search - Function
Proteinase inhibitor I25C, fetuin, conserved site / Fetuin-B-type cystatin domain / Fetuin family signature 1. / Fetuin family signature 2. / Fetuin-B-type cystatin domain profile. / Meprin alpha/beta subunit / Meprin peptidase domain / Astacin-like domain profile. / Peptidase M12A / Astacin (Peptidase family M12A) ...Proteinase inhibitor I25C, fetuin, conserved site / Fetuin-B-type cystatin domain / Fetuin family signature 1. / Fetuin family signature 2. / Fetuin-B-type cystatin domain profile. / Meprin alpha/beta subunit / Meprin peptidase domain / Astacin-like domain profile. / Peptidase M12A / Astacin (Peptidase family M12A) / : / TRAF/meprin, MATH domain / MAM domain signature. / Cystatin domain / Cystatin-like domain / Cystatin domain / Domain in meprin, A5, receptor protein tyrosine phosphatase mu (and others) / Cystatin superfamily / MATH/TRAF domain / MATH/TRAF domain profile. / meprin and TRAF homology / MAM domain, meprin/A5/mu / MAM domain / MAM domain profile. / TRAF-like / Peptidase, metallopeptidase / Zinc-dependent metalloprotease / Metallopeptidase, catalytic domain superfamily / EGF-like domain profile. / EGF-like domain / Neutral zinc metallopeptidases, zinc-binding region signature. / Concanavalin A-like lectin/glucanase domain superfamily
Similarity search - Domain/homology
Meprin A subunit beta / Fetuin-B
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsEckhard, U. / Gomis-Ruth, F.X.
Citation
Journal: Proc.Natl.Acad.Sci.USA / Year: 2021
Title: The crystal structure of a 250-kDa heterotetrameric particle explains inhibition of sheddase meprin beta by endogenous fetuin-B.
Authors: Eckhard, U. / Korschgen, H. / von Wiegen, N. / Stocker, W. / Gomis-Ruth, F.X.
#1: Journal: Sci Rep / Year: 2019
Title: The C-terminal region of human plasma fetuin-B is dispensable for the raised-elephant-trunk mechanism of inhibition of astacin metallopeptidases.
Authors: Guevara, T. / Koerschgen, H. / Cuppari, A. / Schmitz, C. / Kuske, M. / Yiallouros, I. / Floehr, J. / Jahnen-Dechent, W. / Stoecker, W. / Gomis-Ruth, F.X.
#2: Journal: Proc Natl Acad Sci U S A / Year: 2012
Title: Structural basis for the sheddase function of human meprin beta metalloproteinase at the plasma membrane.
Authors: Arolas, J.L. / Broder, C. / Jefferson, T. / Guevara, T. / Sterchi, E.E. / Bode, W. / Stoecker, W. / Becker-Pauly, C. / Gomis-Ruth, F.X.
History
DepositionNov 3, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Apr 14, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Meprin A subunit beta
B: Fetuin-B
C: Meprin A subunit beta
D: Fetuin-B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)228,92528
Polymers212,1184
Non-polymers16,80724
Water3,135174
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration, Heterotrameric assembly of dimeric meprin beta (protease) complexed at both active sites with fetuin-B (inhibitor).
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area30450 Å2
ΔGint181 kcal/mol
Surface area81730 Å2
MethodPISA
Unit cell
Length a, b, c (Å)126.66, 88.24, 156.66
Angle α, β, γ (deg.)90, 113.82, 90
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 2 types, 4 molecules ACBD

#1: Protein Meprin A subunit beta / Endopeptidase-2 / Meprin B / N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta / PABA ...Endopeptidase-2 / Meprin B / N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta / PABA peptide hydrolase / PPH beta


Mass: 62468.352 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MEP1B / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q16820, meprin B
#2: Protein Fetuin-B / Fetuin-like protein IRL685


Mass: 43590.547 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Fetub / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q9QXC1

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Sugars , 9 types, 18 molecules

#3: Polysaccharide
alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1203.105 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4[LFucpa1-3][LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/4,7,6/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5][a1122h-1b_1-5][a1122h-1a_1-5]/1-2-1-3-4-4-2/a3-b1_a4-c1_a6-g1_c4-d1_d3-e1_d6-f1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#4: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#5: Polysaccharide alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1040.964 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-6DManpb1-4DGlcpNAcb1-4[LFucpa1-3][LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/4,6,5/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5][a1122h-1b_1-5][a1122h-1a_1-5]/1-2-1-3-4-2/a3-b1_a4-c1_a6-f1_c4-d1_d6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(6+1)][a-D-Manp]{}}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#6: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#7: Polysaccharide alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D- ...alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 2045.809 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpa1-3DManpa1-2DManpa1-2DManpa1-3[DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/4,12,11/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5][a2122h-1a_1-5]/1-1-2-3-3-3-4-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-h1_d2-e1_e2-f1_f3-g1_h3-i1_h6-k1_i2-j1_k2-l1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(3+1)][a-D-Glcp]{}}}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#8: Polysaccharide alpha-L-fucopyranose-(1-3)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 513.490 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
LFucpa1-3[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2-2/a3-b1_a6-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#9: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 732.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4[LFucpa1-3]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5][a1122h-1b_1-5]/1-2-1-3/a3-b1_a4-c1_c4-d1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#10: Polysaccharide alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta- ...alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
LFucpa1-3[DGlcpNAcb1-4]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2-1/a3-b1_a4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#14: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 4 types, 180 molecules

#11: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Na
#12: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#13: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#15: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 174 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.51 Å3/Da / Density % sol: 64.96 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9.3
Details: 20.0% PEG smear medium, 0.1M ammonium sulfate, 0.05M magnesium sulfate heptahydrate, 0.1M bicine pH 9.3, 10% ethylene glycol.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å
DetectorType: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Aug 11, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9763 Å / Relative weight: 1
ReflectionResolution: 2.8→76.3 Å / Num. obs: 73323 / % possible obs: 93.6 % / Redundancy: 6.9 % / Biso Wilson estimate: 76.1 Å2 / CC1/2: 0.998 / Net I/σ(I): 9.2
Reflection shellResolution: 2.8→2.97 Å / Num. measured obs: 509432 / Num. unique obs: 73323 / CC1/2: 0.573 / % possible all: 94.9

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Processing

Software
NameVersionClassification
BUSTER2.10.3refinement
XDSdata reduction
XSCALEdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4GWM

4gwm


Resolution: 2.8→76.26 Å / Cor.coef. Fo:Fc: 0.892 / Cor.coef. Fo:Fc free: 0.903 / SU R Cruickshank DPI: 0.598 / Cross valid method: FREE R-VALUE / SU R Blow DPI: 0.559 / SU Rfree Blow DPI: 0.314 / SU Rfree Cruickshank DPI: 0.324
RfactorNum. reflection% reflectionSelection details
Rfree0.2607 744 -RANDOM
Rwork0.2363 ---
obs0.2366 72579 93.8 %-
Displacement parametersBiso mean: 122.07 Å2
Baniso -1Baniso -2Baniso -3
1--41.9545 Å20 Å223.4749 Å2
2--24.1487 Å20 Å2
3---17.8058 Å2
Refine analyzeLuzzati coordinate error obs: 0.55 Å
Refinement stepCycle: LAST / Resolution: 2.8→76.26 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12628 0 1116 174 13918
Refine LS restraints
Refine-IDTypeDev idealNumberRestraint functionWeight
X-RAY DIFFRACTIONt_bond_d0.00814157HARMONIC2
X-RAY DIFFRACTIONt_angle_deg119454HARMONIC2
X-RAY DIFFRACTIONt_dihedral_angle_d6408SINUSOIDAL2
X-RAY DIFFRACTIONt_gen_planes2323HARMONIC5
X-RAY DIFFRACTIONt_it14157HARMONIC10
X-RAY DIFFRACTIONt_chiral_improper_torsion2196SEMIHARMONIC5
X-RAY DIFFRACTIONt_utility_distance24HARMONIC1
X-RAY DIFFRACTIONt_ideal_dist_contact10403SEMIHARMONIC4
X-RAY DIFFRACTIONt_omega_torsion3.08
X-RAY DIFFRACTIONt_other_torsion3.11
LS refinement shellResolution: 2.8→2.83 Å
RfactorNum. reflection% reflection
Rfree0.3053 16 -
Rwork0.316 --
obs0.3159 1982 87.2 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.41010.79560.45954.6557-0.76753.83810.17960.11370.03480.11370.00980.48090.03480.4809-0.18940.46570.1152-0.1897-0.2692-0.1102-0.25327.2787-15.997313.548
26.6017-0.5568-1.08784.4419-0.08456.40890.00960.0172-0.5290.01720.0407-0.1101-0.529-0.1101-0.05030.7828-0.0271-0.3392-0.3810.011-0.24412.567818.948923.8415
33.3127-0.4232.15936.0663-0.3085.16670.0176-0.35470.003-0.35470.111-0.12860.003-0.1286-0.12870.4827-0.0161-0.2538-0.44330.0035-0.27913.9050.79863.1618
411.5722-1.6208-2.91152.387-0.93984.8045-0.1962-1.1170.9808-1.117-0.15820.8220.98080.8220.35440.8830.06740.40790.1615-0.36080.442749.7267-35.1935-12.2945
510.2488-12.88362.880907.03791.8094-0.6791-0.9236-0.1913-0.92360.21550.8686-0.19130.86860.46360.6934-0.1255-0.2609-0.4715-0.0739-0.1231.8708-25.84717.2297
67.4233-0.1023-2.78429.10411.20248.4375-0.4272-1.27550.491-1.27550.2404-0.60230.491-0.60230.18680.8719-0.2348-0.2775-0.0256-0.0852-0.211518.1307-28.5717-13.2106
78.329-0.4827-4.65743.43771.006-0.0994-0.15870.42020.45560.42020.03790.61690.45560.61690.12080.75910.19240.17560.1549-0.1160.5253.439-41.4837-7.8386
82.59950.88862.51480-0.37558.08540.5123-2.5231-1.1085-2.5231-0.18860.7523-1.10850.7523-0.3238-0.0921-0.2740.223-0.14920.0232-0.15840.057514.146142.413
97.1708-0.0706-0.52665.5133-0.9255.63340.10670.04320.4570.0432-0.0329-0.15310.457-0.1531-0.07380.72290.0328-0.3499-0.3863-0.0465-0.284422.4155-20.712546.3578
101.8809-0.20141.0396.675-0.86056.38870.07240.318-0.03460.3180.1149-0.0722-0.0346-0.0722-0.18740.61630.0426-0.178-0.4465-0.0159-0.329231.8796-2.559366.6824
114.5446-1.9699-5.3720-2.08631.65160.08630.2011-0.50.2011-0.33081.5687-0.51.56870.24450.8463-0.5244-0.07070.8726-0.15910.807574.008331.677742.5729
124.113.8534-4.99820.0001-5.02911.60670.349-0.38310.5659-0.38310.37030.83870.56590.8387-0.71930.6405-0.097-0.2226-0.4609-0.1015-0.282147.859324.002643.2616
1311.14820.9469-0.0857.10851.15427.0314-0.33370.2825-0.50730.28250.40061.063-0.50731.063-0.06690.72970.0288-0.28-0.002-0.1856-0.090653.712426.549266.9808
146.79271.6704-3.36142.74561.48190.54750.0209-0.3486-0.3671-0.34860.01070.3459-0.36710.3459-0.03170.851-0.35970.03430.69020.15320.389973.397940.72236.4708
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1{ A|62 - A|256 A|702}A62 - 256
2X-RAY DIFFRACTION1{ A|62 - A|256 A|702}A702
3X-RAY DIFFRACTION2{ A|257 - A|427 A|701 }A257 - 427
4X-RAY DIFFRACTION2{ A|257 - A|427 A|701 }A701
5X-RAY DIFFRACTION3{ A|428 - A|593}A428 - 593
6X-RAY DIFFRACTION4{ B|30 - B|145}B30 - 145
7X-RAY DIFFRACTION5{ B|146 - B|160 }B146 - 160
8X-RAY DIFFRACTION6{ B|161 - B|267 }B161 - 267
9X-RAY DIFFRACTION7{ B|303 - B|383 }B303 - 383
10X-RAY DIFFRACTION8{ C|62 - C|256 C|702}C62 - 256
11X-RAY DIFFRACTION8{ C|62 - C|256 C|702}C702
12X-RAY DIFFRACTION9{ C|257 - C|427 C|701 }C257 - 427
13X-RAY DIFFRACTION9{ C|257 - C|427 C|701 }C701
14X-RAY DIFFRACTION10{ C|428 - C|593 }C428 - 593
15X-RAY DIFFRACTION11{ D|30 - D|145 }D30 - 145
16X-RAY DIFFRACTION12{ D|146 - D|160 }D146 - 160
17X-RAY DIFFRACTION13{ D|161 - D|267 }D161 - 267
18X-RAY DIFFRACTION14{ D|304 - D|379 }D304 - 379

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