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- PDB-7amk: Zebrafish RET Cadherin Like Domains 1 to 4. -

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Basic information

Entry
Database: PDB / ID: 7amk
TitleZebrafish RET Cadherin Like Domains 1 to 4.
ComponentsProto-oncogene tyrosine-protein kinase receptor Ret
KeywordsSIGNALING PROTEIN / Ligand recognition / receptor tyrosine kinase / glycosylation
Function / homology
Function and homology information


branchiomeric skeletal muscle development / pronephros morphogenesis / neural crest cell migration involved in autonomic nervous system development / enteric nervous system development / axon extension / homophilic cell-cell adhesion / transmembrane receptor protein tyrosine kinase activity / endomembrane system / cell surface receptor protein tyrosine kinase signaling pathway / receptor protein-tyrosine kinase ...branchiomeric skeletal muscle development / pronephros morphogenesis / neural crest cell migration involved in autonomic nervous system development / enteric nervous system development / axon extension / homophilic cell-cell adhesion / transmembrane receptor protein tyrosine kinase activity / endomembrane system / cell surface receptor protein tyrosine kinase signaling pathway / receptor protein-tyrosine kinase / neuron projection development / protein-containing complex assembly / receptor complex / axon / calcium ion binding / protein homodimerization activity / ATP binding / plasma membrane
Similarity search - Function
Tyrosine-protein kinase, Ret receptor / Tyrosine-protein kinase receptor Ret, cadherin like domain 3 / Ret, cadherin like domain 1 / RET, cadherin-like domain 4 / : / RET Cadherin like domain 1 / RET Cadherin like domain 3 / RET Cadherin like domain 4 / RET, Cysteine Rich Domain / Cadherin repeats. ...Tyrosine-protein kinase, Ret receptor / Tyrosine-protein kinase receptor Ret, cadherin like domain 3 / Ret, cadherin like domain 1 / RET, cadherin-like domain 4 / : / RET Cadherin like domain 1 / RET Cadherin like domain 3 / RET Cadherin like domain 4 / RET, Cysteine Rich Domain / Cadherin repeats. / Cadherin domain / Cadherin-like / Cadherins domain profile. / Cadherin-like superfamily / : / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
DI(HYDROXYETHYL)ETHER / TRIETHYLENE GLYCOL / Proto-oncogene tyrosine-protein kinase receptor Ret
Similarity search - Component
Biological speciesDanio rerio (zebrafish)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å
AuthorsPurkiss, A.G. / McDonald, N.Q. / Goodman, K.M. / Narowtek, A. / Knowles, P.P.
CitationJournal: Structure / Year: 2021
Title: A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing.
Authors: Sarah E Adams / Andrew G Purkiss / Phillip P Knowles / Andrea Nans / David C Briggs / Annabel Borg / Christopher P Earl / Kerry M Goodman / Agata Nawrotek / Aaron J Borg / Pauline B McIntosh ...Authors: Sarah E Adams / Andrew G Purkiss / Phillip P Knowles / Andrea Nans / David C Briggs / Annabel Borg / Christopher P Earl / Kerry M Goodman / Agata Nawrotek / Aaron J Borg / Pauline B McIntosh / Francesca M Houghton / Svend Kjær / Neil Q McDonald /
Abstract: RET receptor tyrosine kinase plays vital developmental and neuroprotective roles in metazoans. GDNF family ligands (GFLs) when bound to cognate GFRα co-receptors recognize and activate RET ...RET receptor tyrosine kinase plays vital developmental and neuroprotective roles in metazoans. GDNF family ligands (GFLs) when bound to cognate GFRα co-receptors recognize and activate RET stimulating its cytoplasmic kinase function. The principles for RET ligand-co-receptor recognition are incompletely understood. Here, we report a crystal structure of the cadherin-like module (CLD1-4) from zebrafish RET revealing interdomain flexibility between CLD2 and CLD3. Comparison with a cryo-electron microscopy structure of a ligand-engaged zebrafish RET-GDNF-GFRα1a complex indicates conformational changes within a clade-specific CLD3 loop adjacent to the co-receptor. Our observations indicate that RET is a molecular clamp with a flexible calcium-dependent arm that adapts to different GFRα co-receptors, while its rigid arm recognizes a GFL dimer to align both membrane-proximal cysteine-rich domains. We also visualize linear arrays of RET-GDNF-GFRα1a suggesting that a conserved contact stabilizes higher-order species. Our study reveals that ligand-co-receptor recognition by RET involves both receptor plasticity and strict spacing of receptor dimers by GFL ligands.
History
DepositionOct 9, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 3, 2021Provider: repository / Type: Initial release
Revision 1.1Jul 14, 2021Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first
Revision 1.2Jan 31, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.3Oct 16, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Proto-oncogene tyrosine-protein kinase receptor Ret
B: Proto-oncogene tyrosine-protein kinase receptor Ret
hetero molecules


Theoretical massNumber of molelcules
Total (without water)118,65640
Polymers109,2212
Non-polymers9,43538
Water3,945219
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: SAXS, Some evidence of solution dimer for construct under crystallographic conditions.
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area17760 Å2
ΔGint142 kcal/mol
Surface area47420 Å2
MethodPISA
Unit cell
Length a, b, c (Å)51.167, 70.499, 105.438
Angle α, β, γ (deg.)105.408, 100.934, 100.253
Int Tables number1
Space group name H-MP1
Space group name HallP1
Symmetry operation#1: x,y,z
Noncrystallographic symmetry (NCS)NCS oper: (Code: givenMatrix: (0.430793954191, -0.900333557273, -0.0617742234261), (-0.902265964183, -0.431079226797, -0.00931826708919), (-0.0182400359145, 0.0597510323868, -0.998046649821)Vector: ...NCS oper: (Code: given
Matrix: (0.430793954191, -0.900333557273, -0.0617742234261), (-0.902265964183, -0.431079226797, -0.00931826708919), (-0.0182400359145, 0.0597510323868, -0.998046649821)
Vector: 10.4488119906, 11.910090856, 98.3978260294)

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Proto-oncogene tyrosine-protein kinase receptor Ret


Mass: 54610.270 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Danio rerio (zebrafish) / Gene: ret, ret1 / Production host: unidentified baculovirus
References: UniProt: A8E7C6, receptor protein-tyrosine kinase

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Sugars , 7 types, 12 molecules

#2: Polysaccharide alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D- ...alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1219.105 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-6DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/4,7,6/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5][a1221m-1a_1-5]/1-1-2-3-3-3-4/a4-b1_a6-g1_b4-c1_c3-d1_c6-f1_d6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(6+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{}}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#3: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#4: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#5: Polysaccharide alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 894.823 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-6DManpb1-4DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/4,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5][a1221m-1a_1-5]/1-1-2-3-4/a4-b1_a6-e1_b4-c1_c6-d1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(6+1)][a-D-Manp]{}}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#6: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 732.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1221m-1a_1-5]/1-1-2-3/a4-b1_a6-d1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#7: Polysaccharide alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 894.823 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3DManpb1-4DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/4,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5][a1221m-1a_1-5]/1-1-2-3-4/a4-b1_a6-e1_b4-c1_c3-d1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#9: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 6 types, 245 molecules

#8: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Formula: Ca
#10: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Formula: C3H8O3
#11: Chemical
ChemComp-PGE / TRIETHYLENE GLYCOL


Mass: 150.173 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Formula: C6H14O4
#12: Chemical
ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 10
Source method: isolated from a genetically manipulated source
Formula: C4H10O3
#13: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Formula: C6H13NO4S / Comment: pH buffer*YM
#14: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 219 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.28 Å3/Da / Density % sol: 62.53 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.2 / Details: 50 mM MES (pH 6.2), 31.5 % PEG MME 350 (v/v)

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9787 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 24, 2015 / Details: Mirrors
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9787 Å / Relative weight: 1
ReflectionResolution: 2.08→65.96 Å / Num. obs: 79868 / % possible obs: 98.2 % / Redundancy: 3.4 % / Biso Wilson estimate: 28.87 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.08 / Rpim(I) all: 0.051 / Rrim(I) all: 0.095 / Net I/σ(I): 6.2
Reflection shellResolution: 2.08→2.19 Å / Redundancy: 3.3 % / Rmerge(I) obs: 1.186 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 11549 / CC1/2: 0.58 / Rpim(I) all: 0.765 / Rrim(I) all: 1.416 / % possible all: 97.2

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Processing

Software
NameVersionClassification
PHENIXdev_3965refinement
DIALSdata reduction
STARANISOdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2x2u

2x2u


Resolution: 2.2→65.96 Å / SU ML: 0.3521 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 29.5782
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2694 3097 4.94 %
Rwork0.2251 59574 -
obs0.2273 62671 91.28 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 39.39 Å2
Refinement stepCycle: LAST / Resolution: 2.2→65.96 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7341 0 609 219 8169
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00828107
X-RAY DIFFRACTIONf_angle_d0.888211020
X-RAY DIFFRACTIONf_chiral_restr0.05091392
X-RAY DIFFRACTIONf_plane_restr0.00611348
X-RAY DIFFRACTIONf_dihedral_angle_d14.37973021
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.2-2.230.3631940.30281821X-RAY DIFFRACTION62.62
2.23-2.270.38941360.30322040X-RAY DIFFRACTION69.74
2.27-2.310.34491180.30132075X-RAY DIFFRACTION70.76
2.31-2.350.35191250.28372336X-RAY DIFFRACTION77.66
2.35-2.40.31181210.28182419X-RAY DIFFRACTION82.15
2.4-2.450.34141350.28182532X-RAY DIFFRACTION85.13
2.45-2.50.29071410.28292658X-RAY DIFFRACTION90.38
2.5-2.560.31771440.27482823X-RAY DIFFRACTION93.04
2.56-2.620.34141680.27342812X-RAY DIFFRACTION96.66
2.62-2.690.31831530.26512963X-RAY DIFFRACTION98.08
2.69-2.770.32481470.24292865X-RAY DIFFRACTION98.08
2.77-2.860.28261620.24292910X-RAY DIFFRACTION98.12
2.86-2.960.31231700.24522890X-RAY DIFFRACTION98.42
2.96-3.080.28541660.24052923X-RAY DIFFRACTION98.47
3.08-3.220.32081340.22962929X-RAY DIFFRACTION98.17
3.22-3.390.26811470.21142927X-RAY DIFFRACTION98.53
3.39-3.60.2621520.21142903X-RAY DIFFRACTION98.04
3.61-3.880.27941380.20452940X-RAY DIFFRACTION98.5
3.88-4.270.19771210.19492972X-RAY DIFFRACTION98.66
4.27-4.890.19771360.17492931X-RAY DIFFRACTION98.78
4.89-6.160.21811380.19562961X-RAY DIFFRACTION98.95
6.16-65.960.2111510.20892944X-RAY DIFFRACTION98.95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.67583578694-0.7106266100210.6173712635011.80309617827-0.6215578450381.93664887926-0.107893129509-0.0813318220143-0.1241992065410.4045109035030.0167450588865-0.2343409455050.155661752767-0.04550556787510.06329567534570.197774497034-0.01669342183530.005483044752960.1359775929760.002212263255770.277877518367-12.515379231620.7123630395105.651400189
20.781527938321-0.1225577359550.5998185710642.12267378881.866045016636.560475175690.04142900584180.0613756473978-0.0364462846596-0.3845941414280.03320243280660.0186544734530.371836698742-0.335893091342-0.063965335810.308542649105-0.095297770939-0.001468110083450.256304499434-0.01229228220180.255915739034-29.568889357912.271553382159.8677776744
35.499727301581.48125889753-3.264419534411.24728902399-1.216595816096.431612367250.0692627486091-0.05229433060040.182395024620.1095013912940.236217267095-0.3952250589560.006212978296920.871674241071-0.2891650795310.3850238220530.0131443113195-0.03392663993480.322316425893-0.1642439073710.518957203553-11.52902379089.1576402799118.9450518331
41.481926396830.09787563134470.1012316159742.470614677361.002552950711.8998600338-0.1375274629930.235546782360.130465968854-0.09938142816260.142193454258-0.229373793223-0.1876093584720.153135545477-0.02029115800120.218484720283-0.0388226031303-0.01623046125730.1361266882190.04650500870450.231277690607-21.115011216413.6997875187-5.79528238352
51.201467960780.04012458568341.525053896490.235656952728-0.4016269025627.44908799659-0.13024220282-0.121043580254-0.110214792030.00782327482205-0.101055305250.013738411519-0.2620695337310.3541330177620.2157917847970.4645848450620.0220370511128-0.03735684969880.242331337250.0582935124920.27592163663-16.393276684434.552815626239.2359442659
62.14574681612-1.309861832611.839533522313.56153550555-2.115471685484.683818506090.03316641507990.12694403795-0.225550876227-0.0805431063961-0.0869616177737-0.09400225672590.2645844827690.2264477913650.05909724240780.1274393028550.02639084718830.05518353346380.200936468876-0.03252591439750.222810624532-5.1046867068317.873891159279.6055540559
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and ((resid 23 through 250 ) or resid 801 or resid 806 or ( resid 811 through 818) or resid 861)AC - T23 - 8614
22chain 'A' and ((resid 251 through 383 ) or (resid 821 through 822) or (resid 831 through 832) or (resid 841 through 842) or resid 891)AC - U251 - 891232
33chain 'A' and ((resid 384 through 498 ) or (resid 851 through 852) or resid 892)AC - V384 - 892365
44chain 'B' and ((resid 23 through 250 ) or resid 806 or ( resid 811 through 814) or resid 861)BY - A23 - 8614
55chain 'B' and ((resid 251 through 383 ) or (resid 831 through 832) or (resid 841 through 843) or resid 891)BY - A251 - 843232
66chain 'B' and ((resid 384 through 498 ) or (resid 851 through 852))BY - A384 - 852361

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