Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 / SH3 and multiple ankyrin repeat domains protein 1 Similarity search - Component
Biological species
Rattus norvegicus (Norway rat) Homo sapiens (human)
A: SH3 and multiple ankyrin repeat domains protein 1,Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 B: SH3 and multiple ankyrin repeat domains protein 1,Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 hetero molecules
Mass: 18.015 Da / Num. of mol.: 122 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
N
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.01 Å3/Da / Density % sol: 59.14 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 100 nL of protein solution (15 mg/ml mutant protein in 10 mM HEPES buffer, pH 8.0, 150 mM NaCl, 5% (v/v) glycerol and 0.5 mM TCEP) were mixed with 100 nL reservoir buffer (16% (w/v) ...Details: 100 nL of protein solution (15 mg/ml mutant protein in 10 mM HEPES buffer, pH 8.0, 150 mM NaCl, 5% (v/v) glycerol and 0.5 mM TCEP) were mixed with 100 nL reservoir buffer (16% (w/v) polyethylene glycol 3350 and 0.2 M ammonium citrate), above a reservoir volume of 20 microL
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N