- PDB-7a2d: Structure-function analyses of dual-BON domain protein DolP ident... -
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Basic information
Entry
Database: PDB / ID: 7a2d
Title
Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation to the cell division site
BMRB: 34552Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation to the cell division site
Text: THE STRUCTURE WAS CALCULATED USING TRADITIONAL NOE AND DEHEDRAL ANGLE RESTRAINTS
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Sample preparation
Details
Type: solution Contents: 1.5 mM [U-13C; U-15N] DolP, 50 mM NA sodium phosphate, 0.02 % w/v NA NaN3, 50 mM NA sodium chloride, 90% H2O/10% D2O Details: 1.5 mM 13C-15N labelled DolP in 50 mM sodium phosphate (pH 6), 50 mM NaCl and 0.02% NaN3 in 90% H2O/10% D2O. Label: 13C-15N-sample / Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
1.5mM
DolP
[U-13C; U-15N]
1
50mM
sodiumphosphate
NA
1
0.02 % w/v
NaN3
NA
1
50mM
sodiumchloride
NA
1
Sample conditions
Ionic strength: 0.05 M / Label: conditions_1 / pH: 6 / PH err: 0.05 / Pressure: AMBIENT bar / Temperature: 298 K
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