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- PDB-6znk: MaeB PTA domain N718D mutant -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 6znk
TitleMaeB PTA domain N718D mutant
ComponentsMalate dehydrogenase
KeywordsOXIDOREDUCTASE / malic enzyme
Function / homology
Function and homology information


malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) / malate dehydrogenase (decarboxylating) (NADP+) activity / oxaloacetate decarboxylase activity / acyltransferase activity / NAD binding / metal ion binding
Similarity search - Function
Malic enzyme, NAD-binding domain, bacterial type / : / Phosphate acetyltransferase, domain 2 / Phosphate acetyltransferase, domain 1 / Phosphate acetyl/butaryl transferase / Phosphate acetyl/butaryl transferase / Malic enzyme, N-terminal domain / Malic enzyme, NAD-binding / Malic enzyme, N-terminal domain superfamily / Malic enzyme, N-terminal domain ...Malic enzyme, NAD-binding domain, bacterial type / : / Phosphate acetyltransferase, domain 2 / Phosphate acetyltransferase, domain 1 / Phosphate acetyl/butaryl transferase / Phosphate acetyl/butaryl transferase / Malic enzyme, N-terminal domain / Malic enzyme, NAD-binding / Malic enzyme, N-terminal domain superfamily / Malic enzyme, N-terminal domain / Malic enzyme, NAD binding domain / Malic enzyme, NAD binding domain / Aminoacid dehydrogenase-like, N-terminal domain superfamily / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
Malate dehydrogenase
Similarity search - Component
Biological speciesBdellovibrio bacteriovorus (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.039 Å
AuthorsLovering, A.L. / Harding, C.J.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Biotechnology and Biological Sciences Research Council (BBSRC)mibtp studentship United Kingdom
CitationJournal: Nat Commun / Year: 2021
Title: A rotary mechanism for allostery in bacterial hybrid malic enzymes.
Authors: Harding, C.J. / Cadby, I.T. / Moynihan, P.J. / Lovering, A.L.
History
DepositionJul 6, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 17, 2021Provider: repository / Type: Initial release
Revision 2.0Mar 17, 2021Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Database references / Derived calculations / Experimental preparation / Non-polymer description / Other / Polymer sequence / Source and taxonomy / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / cell / chem_comp / citation / citation_author / entity / entity_poly / entity_poly_seq / entity_src_gen / exptl_crystal / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_poly_seq_scheme / pdbx_struct_sheet_hbond / pdbx_unobs_or_zero_occ_atoms / pdbx_unobs_or_zero_occ_residues / struct_asym / struct_conf / struct_mon_prot_cis / struct_ref / struct_ref_seq / struct_ref_seq_dif / struct_sheet_range / struct_site_gen
Item: _atom_site.label_entity_id / _atom_site.label_seq_id ..._atom_site.label_entity_id / _atom_site.label_seq_id / _atom_site_anisotrop.pdbx_label_seq_id / _cell.Z_PDB / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.name / _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _exptl_crystal.density_Matthews / _exptl_crystal.density_percent_sol / _pdbx_entity_nonpoly.entity_id / _pdbx_nonpoly_scheme.entity_id / _pdbx_struct_sheet_hbond.range_1_label_seq_id / _pdbx_struct_sheet_hbond.range_2_label_seq_id / _pdbx_unobs_or_zero_occ_atoms.label_seq_id / _struct_asym.entity_id / _struct_conf.beg_label_seq_id / _struct_conf.end_label_seq_id / _struct_mon_prot_cis.label_seq_id / _struct_mon_prot_cis.pdbx_label_seq_id_2 / _struct_ref_seq.db_align_beg / _struct_ref_seq.pdbx_auth_seq_align_beg / _struct_ref_seq.ref_id / _struct_ref_seq.seq_align_beg / _struct_ref_seq.seq_align_end / _struct_sheet_range.beg_label_seq_id / _struct_sheet_range.end_label_seq_id / _struct_site_gen.label_seq_id
Revision 2.1Jan 31, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Malate dehydrogenase
B: Malate dehydrogenase
D: Malate dehydrogenase
F: Malate dehydrogenase
E: Malate dehydrogenase
C: Malate dehydrogenase
G: Malate dehydrogenase
H: Malate dehydrogenase
I: Malate dehydrogenase
J: Malate dehydrogenase
K: Malate dehydrogenase
L: Malate dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)476,99636
Polymers474,69012
Non-polymers2,30624
Water00
1
A: Malate dehydrogenase
B: Malate dehydrogenase
D: Malate dehydrogenase
F: Malate dehydrogenase
E: Malate dehydrogenase
C: Malate dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)238,49818
Polymers237,3456
Non-polymers1,15312
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
G: Malate dehydrogenase
H: Malate dehydrogenase
I: Malate dehydrogenase
J: Malate dehydrogenase
K: Malate dehydrogenase
L: Malate dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)238,49818
Polymers237,3456
Non-polymers1,15312
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)139.607, 150.807, 282.059
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11chain A
21chain B
31(chain C and resid 439 through 776)
41(chain D and resid 439 through 776)
51chain E
61chain F
71chain G
81chain H
91chain I
101chain J
111chain K
121chain L

NCS domain segments:

Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: SER / Beg label comp-ID: SER / End auth comp-ID: SER / End label comp-ID: SER / Auth seq-ID: 439 - 776 / Label seq-ID: 21 - 358

Dom-IDSelection detailsAuth asym-IDLabel asym-ID
1chain AAA
2chain BBB
3(chain C and resid 439 through 776)CF
4(chain D and resid 439 through 776)DC
5chain EEE
6chain FFD
7chain GGG
8chain HHH
9chain III
10chain JJJ
11chain KKK
12chain LLL

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Components

#1: Protein
Malate dehydrogenase


Mass: 39557.539 Da / Num. of mol.: 12 / Mutation: N718D
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100) (bacteria)
Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100 / Gene: mdh, Bd1833 / Variant: N718D mutant / Production host: Escherichia coli (E. coli)
References: UniProt: Q6MM15, malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
#2: Chemical...
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 24 / Source method: obtained synthetically / Formula: SO4 / References: UniProt: Q6MM15*PLUS
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.13 Å3/Da / Density % sol: 60.67 %
Crystal growTemperature: 289 K / Method: vapor diffusion / pH: 7 / Details: 0.1M Hepes pH 7.0 20% PEG 8000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Dec 7, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.039→102.46 Å / Num. obs: 115105 / % possible obs: 100 % / Redundancy: 12.7 % / CC1/2: 0.99 / Net I/σ(I): 10.5
Reflection shellResolution: 3.04→3.1 Å / Mean I/σ(I) obs: 1.14 / Num. unique obs: 8421 / CC1/2: 0.63

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Processing

Software
NameVersionClassification
Aimlessdata scaling
PHENIX1.14_3260refinement
PDB_EXTRACT3.25data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1TD9

1td9


Resolution: 3.039→79.784 Å / SU ML: 0.42 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 27.86 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2528 5575 4.86 %
Rwork0.2036 109063 -
obs0.206 114638 99.6 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 259.66 Å2 / Biso mean: 100.2243 Å2 / Biso min: 47.31 Å2
Refinement stepCycle: final / Resolution: 3.039→79.784 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms31197 0 120 0 31317
Biso mean--109.81 --
Num. residues----4059
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A19878X-RAY DIFFRACTION11.745TORSIONAL
12B19878X-RAY DIFFRACTION11.745TORSIONAL
13C19878X-RAY DIFFRACTION11.745TORSIONAL
14D19878X-RAY DIFFRACTION11.745TORSIONAL
15E19878X-RAY DIFFRACTION11.745TORSIONAL
16F19878X-RAY DIFFRACTION11.745TORSIONAL
17G19878X-RAY DIFFRACTION11.745TORSIONAL
18H19878X-RAY DIFFRACTION11.745TORSIONAL
19I19878X-RAY DIFFRACTION11.745TORSIONAL
110J19878X-RAY DIFFRACTION11.745TORSIONAL
111K19878X-RAY DIFFRACTION11.745TORSIONAL
112L19878X-RAY DIFFRACTION11.745TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.0391-3.07370.3621550.3023354898
3.0737-3.10980.35991890.30953622100
3.1098-3.14780.31021900.30513577100
3.1478-3.18760.34971910.2973570100
3.1876-3.22960.32451860.27553656100
3.2296-3.27380.29871750.2613622100
3.2738-3.32060.30921840.26753611100
3.3206-3.37010.28141790.26273610100
3.3701-3.42280.32471860.26373652100
3.4228-3.47890.29812030.27023562100
3.4789-3.53890.29821980.28083595100
3.5389-3.60330.36191690.28663640100
3.6033-3.67260.38521500.3667361899
3.6726-3.74750.46141840.3631349697
3.7475-3.8290.321740.25353606100
3.829-3.91810.26571750.21263639100
3.9181-4.01610.2851790.22053633100
4.0161-4.12460.25211630.21043647100
4.1246-4.2460.24682000.19963613100
4.246-4.3830.22251980.17013636100
4.383-4.53970.19211840.16033634100
4.5397-4.72140.19571990.1533627100
4.7214-4.93630.21851760.15653656100
4.9363-5.19650.2381800.16413697100
5.1965-5.5220.26552030.19223641100
5.522-5.94820.27531950.2073669100
5.9482-6.54660.26052330.18173670100
6.5466-7.49330.21722080.16553684100
7.4933-9.43830.16341730.12743798100
9.4383-79.7840.20481960.1721383498
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.20381.19660.53564.49610.84834.01840.4186-0.2959-0.16241.0016-0.3375-0.37690.18190.4899-0.07040.6933-0.2705-0.18161.12940.06220.690114.763728.882658.5536
22.99320.3779-0.5652.73560.66864.44760.0585-0.0243-0.20220.0270.1205-0.46230.34481.1706-0.16670.49810.0982-0.01311.2691-0.09250.734916.455713.437832.0444
30.4498-0.5525-0.64733.724-1.02363.5998-0.2431.0497-0.25760.0220.4182-0.91320.85140.6323-0.14240.5754-0.1087-0.15241.2697-0.15210.925719.137321.900151.6267
42.1512-0.51452.16283.0708-0.18185.45670.28870.284-0.3843-0.1949-0.03870.12451.40910.3759-0.17620.9626-0.02790.030.7502-0.17220.8645-6.8556-5.00637.9107
53.9291-1.3042-0.29894.65561.03614.4154-0.0917-0.2094-0.23930.40120.09440.42790.34380.0825-0.00070.5768-0.01910.09840.69090.00940.6936-7.315511.079834.17
61.40380.2939-0.4664.0888-0.98043.847-0.14460.4115-0.01860.38240.1106-0.07080.7724-0.2832-0.01870.8029-0.03840.05050.8371-0.16390.7891-9.9241-2.838617.9374
71.45420.4593-2.09772.91551.4615.29520.25760.40970.1744-0.80140.1190.1107-0.7789-0.5107-0.34090.88790.071-0.05471.01420.23050.8666-16.0964.4465-11.6656
82.62150.1148-0.72083.95190.20761.1663-0.04390.3596-0.3008-0.55150.11040.64510.4377-0.3875-0.07870.841-0.2213-0.17510.81150.00050.7598-11.547933.9554-11.1184
90.83490.1130.35582.86080.39893.91110.3296-0.6419-0.7656-0.7425-0.2770.5707-0.0096-0.357-0.03950.7087-0.0518-0.16681.06210.16880.8444-17.93254.2-14.5487
100.58090.9-0.17055.1108-0.63123.78080.2985-0.53840.1830.8822-0.10680.1086-0.57230.1437-0.20580.6908-0.15950.1721.0813-0.09980.7115-14.034146.58858.6014
113.3510.07420.56092.6826-0.78685.19030.1350.05930.25240.2451-0.02620.3723-0.4677-0.4917-0.10830.5921-0.03540.07630.74320.0150.7754-16.204160.766231.4938
120.5819-1.4203-0.47694.2731-0.06644.06260.4707-0.4940.65830.0971-0.16560.6457-0.99890.2708-0.36090.7769-0.06360.19430.9736-0.05080.7758-18.55453.169451.4745
131.794-0.0263-2.45442.47150.00115.62860.65990.2310.581-0.0098-0.0741-0.0417-1.7707-0.1027-0.4341.086-0.02550.19360.71560.13680.87266.790478.5426.2922
143.9429-0.665-0.46384.0873-0.41173.73490.1759-0.3870.24960.5556-0.1976-0.4072-0.49520.66330.06830.6328-0.2463-0.12060.77340.01880.62537.612963.418833.0564
155.71531.9711.06393.80341.33544.08280.8255-0.337-0.49180.7718-0.0476-0.1667-1.65430.8436-0.62911.0257-0.17950.22870.9890.17270.60610.01776.535316.4075
161.9976-1.68211.88123.9068-2.6783.79920.38510.1234-0.1563-1.0903-0.15820.00340.90380.3896-0.23060.92930.01110.04350.8158-0.20040.765416.7388.6581-11.0916
173.55110.02280.74574.0752-0.01792.41350.11110.42780.1658-0.6312-0.0264-0.5511-0.1510.2832-0.11210.7865-0.11760.18980.7569-0.05450.69111.971839.051-11.4152
182.5850.8544-0.22034.4039-1.11514.93330.23370.29591.1921-1.2762-0.393-0.1516-0.12570.54880.1860.960.14020.09890.9124-0.09140.904518.547918.7035-14.4557
194.1855-0.1253-0.29273.35381.75483.5584-0.17080.3957-0.3104-0.26530.5997-0.44290.43461.0267-0.38080.82560.04860.0550.7565-0.10660.6848-22.5637-24.555850.878
202.89621.18570.20544.8647-0.23235.6155-0.0614-0.2621-0.15950.5650.2131-0.1140.52930.6448-0.10130.54150.2515-0.02670.661-0.00720.4965-22.7294-9.620377.7376
213.9967-1.45010.57143.28890.37623.3568-0.3686-0.13390.20610.76721.0383-0.17630.19780.8115-0.59831.01820.1542-0.0740.8081-0.19690.7286-19.4356-22.827261.0841
224.4102-1.0548-0.4132.97391.43473.5536-0.15-1.0450.64440.53440.5162-0.3976-0.02741.0412-0.280.8393-0.1107-0.07281.1206-0.17210.774-20.378624.69891.4392
234.4151-0.77320.27114.8421-0.08574.3760.00680.53730.0396-0.2829-0.01690.0187-0.25870.52190.04440.4501-0.1258-0.00570.7450.04090.4694-22.16610.197764.5044
245.1382-0.5544-0.21543.17430.20783.3795-0.31580.0592-0.08420.4040.69460.1426-0.69631.1184-0.34960.9007-0.1512-0.050.895-0.1660.7497-17.737922.842281.0176
251.34221.0217-0.71350.96041.11784.5405-0.0507-0.15890.33330.3529-0.81891.44760.3987-1.60440.64640.8261-0.19830.12211.2359-0.47232.0163-92.5628-13.321467.5372
264.7199-0.244-0.95674.3823-0.54454.758-0.09230.2449-0.35180.0594-0.03280.24550.4241-0.34980.09410.6681-0.1944-0.0770.43350.02990.8152-65.9619-20.626954.1967
274.314-0.23410.52942.92412.88843.15420.32740.3632-0.24170.2056-1.8951.46570.9431-0.71.10540.9632-0.24550.19061.1247-0.41031.5272-85.6586-19.828262.6308
284.65231.23781.002-0.0761.11922.72710.41430.5315-0.67810.0562-0.0291-0.00060.47160.3578-0.3611.01930.0994-0.2060.56320.04810.819-41.1547-6.618629.0696
293.49210.3628-0.93693.4450.56935.1404-0.13480.20550.438-0.3441-0.04390.2866-0.4484-0.46670.14950.73270.0042-0.23650.45970.1090.8234-67.16610.951243.6266
302.65480.41631.23341.80371.16412.92180.5170.11440.06290.368-0.43850.26840.2738-0.0711-0.05320.8613-0.0156-0.12460.66350.0760.7993-50.9924-2.385229.7806
311.0796-1.2109-0.12962.13660.3363.6836-0.333-0.19820.1602-0.2785-0.84011.2768-0.635-2.07860.76520.820.3804-0.27721.7621-0.70151.8293-90.929819.657875.0701
324.5866-0.3646-0.64654.87210.81644.22940.1096-0.07980.34740.1333-0.18940.2669-0.415-0.07970.08340.66390.12670.07540.40840.01390.8528-63.848524.438788.9939
331.5168-0.23361.20583.0036-1.36926.0623-0.7609-1.88751.3171-0.4274-0.16390.78740.3634-2.0520.26190.69410.5542-0.39831.4181-0.55861.7523-83.574825.868480.2841
344.7444-0.7542-0.7713-0.05631.09743.73980.4568-0.66570.5322-0.0083-0.0351-0.0091-0.27230.2726-0.33181.03810.04350.21940.45820.04060.7441-40.82178.0862113.3548
353.5351-0.2833-0.38233.10750.38643.9661-0.3905-0.1784-0.40380.59420.05020.45730.8472-0.24530.30090.87910.04150.25590.48530.09150.8292-67.22482.813198.979
363.43220.2768-1.44060.64650.54022.84370.3038-0.36810.33440.43220.02050.6388-0.4629-0.4395-0.32661.09680.20940.07510.67810.0240.7856-50.88734.5277112.6929
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 439 through 595 )A439 - 595
2X-RAY DIFFRACTION2chain 'A' and (resid 596 through 736 )A596 - 736
3X-RAY DIFFRACTION3chain 'A' and (resid 737 through 776 )A737 - 776
4X-RAY DIFFRACTION4chain 'B' and (resid 439 through 595 )B439 - 595
5X-RAY DIFFRACTION5chain 'B' and (resid 596 through 736 )B596 - 736
6X-RAY DIFFRACTION6chain 'B' and (resid 737 through 776 )B737 - 776
7X-RAY DIFFRACTION7chain 'D' and (resid 439 through 595 )D439 - 595
8X-RAY DIFFRACTION8chain 'D' and (resid 596 through 736 )D596 - 736
9X-RAY DIFFRACTION9chain 'D' and (resid 737 through 776 )D737 - 776
10X-RAY DIFFRACTION10chain 'F' and (resid 439 through 595 )F439 - 595
11X-RAY DIFFRACTION11chain 'F' and (resid 596 through 736 )F596 - 736
12X-RAY DIFFRACTION12chain 'F' and (resid 737 through 776 )F737 - 776
13X-RAY DIFFRACTION13chain 'E' and (resid 439 through 595 )E439 - 595
14X-RAY DIFFRACTION14chain 'E' and (resid 596 through 736 )E596 - 736
15X-RAY DIFFRACTION15chain 'E' and (resid 737 through 776 )E737 - 776
16X-RAY DIFFRACTION16chain 'C' and (resid 439 through 595 )C439 - 595
17X-RAY DIFFRACTION17chain 'C' and (resid 596 through 736 )C596 - 736
18X-RAY DIFFRACTION18chain 'C' and (resid 737 through 776 )C737 - 776
19X-RAY DIFFRACTION19chain 'G' and (resid 439 through 595 )G439 - 595
20X-RAY DIFFRACTION20chain 'G' and (resid 596 through 736 )G596 - 736
21X-RAY DIFFRACTION21chain 'G' and (resid 737 through 776 )G737 - 776
22X-RAY DIFFRACTION22chain 'H' and (resid 439 through 595 )H439 - 595
23X-RAY DIFFRACTION23chain 'H' and (resid 596 through 736 )H596 - 736
24X-RAY DIFFRACTION24chain 'H' and (resid 737 through 776 )H737 - 776
25X-RAY DIFFRACTION25chain 'I' and (resid 439 through 595 )I439 - 595
26X-RAY DIFFRACTION26chain 'I' and (resid 596 through 736 )I596 - 736
27X-RAY DIFFRACTION27chain 'I' and (resid 737 through 776 )I737 - 776
28X-RAY DIFFRACTION28chain 'J' and (resid 439 through 595 )J439 - 595
29X-RAY DIFFRACTION29chain 'J' and (resid 596 through 736 )J596 - 736
30X-RAY DIFFRACTION30chain 'J' and (resid 737 through 776 )J737 - 776
31X-RAY DIFFRACTION31chain 'K' and (resid 439 through 595 )K439 - 595
32X-RAY DIFFRACTION32chain 'K' and (resid 596 through 736 )K596 - 736
33X-RAY DIFFRACTION33chain 'K' and (resid 737 through 776 )K737 - 776
34X-RAY DIFFRACTION34chain 'L' and (resid 439 through 595 )L439 - 595
35X-RAY DIFFRACTION35chain 'L' and (resid 596 through 736 )L596 - 736
36X-RAY DIFFRACTION36chain 'L' and (resid 737 through 776 )L737 - 776

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