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Open data
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Basic information
Entry | Database: PDB / ID: 6zn9 | |||||||||
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Title | MaeB PTA domain apoprotein | |||||||||
![]() | Malate dehydrogenase | |||||||||
![]() | OXIDOREDUCTASE / malic enzyme | |||||||||
Function / homology | ![]() malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) / malate dehydrogenase (decarboxylating) (NADP+) activity / oxaloacetate decarboxylase activity / acyltransferase activity / NAD binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Lovering, A.L. / Harding, C.J. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: A rotary mechanism for allostery in bacterial hybrid malic enzymes. Authors: Harding, C.J. / Cadby, I.T. / Moynihan, P.J. / Lovering, A.L. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 751.7 KB | Display | ![]() |
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PDB format | ![]() | 628.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 554.1 KB | Display | ![]() |
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Full document | ![]() | 630.3 KB | Display | |
Data in XML | ![]() | 135.3 KB | Display | |
Data in CIF | ![]() | 183.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6zn4C ![]() 6zn7C ![]() 6zneC ![]() 6zngC ![]() 6znjC ![]() 6znkC ![]() 6znrC ![]() 6zntC ![]() 6znuC ![]() 1td9S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39556.555 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15356 / DSM 50701 / NCIB 9529 / HD100 / Gene: mdh, Bd1833 / Production host: ![]() ![]() References: UniProt: Q6MM15, malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.13 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion / pH: 4.5 Details: 0.1M Sodium Acetate pH 4.5 0.1M Magnesium Acetate 8% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Mar 1, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 2.72→142.66 Å / Num. obs: 162146 / % possible obs: 99.9 % / Redundancy: 12.84 % / CC1/2: 0.99 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 2.72→2.76 Å / Num. unique obs: 7836 / CC1/2: 0.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1TD9 Resolution: 2.72→99.637 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 274.3 Å2 / Biso mean: 85.7841 Å2 / Biso min: 30.67 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.72→99.637 Å
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