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- PDB-6zbu: Crystal structure of an NCoR1BBD2-BCL6BTB chimera in complex with... -

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Basic information

Entry
Database: PDB / ID: 6zbu
TitleCrystal structure of an NCoR1BBD2-BCL6BTB chimera in complex with the NcoR1 BBD1 corepressor peptide
Components
  • Nuclear receptor corepressor 1
  • Nuclear receptor corepressor 1,B-cell lymphoma 6 protein
KeywordsTRANSCRIPTION / BCL6 / NCoR1.
Function / homology
Function and homology information


Loss of MECP2 binding ability to the NCoR/SMRT complex / negative regulation of transcription initiation by RNA polymerase II / negative regulation of androgen receptor signaling pathway / negative regulation of mitotic cell cycle DNA replication / intronic transcription regulatory region sequence-specific DNA binding / regulation of germinal center formation / paraspeckles / negative regulation of B cell apoptotic process / regulation of immune system process / type 2 immune response ...Loss of MECP2 binding ability to the NCoR/SMRT complex / negative regulation of transcription initiation by RNA polymerase II / negative regulation of androgen receptor signaling pathway / negative regulation of mitotic cell cycle DNA replication / intronic transcription regulatory region sequence-specific DNA binding / regulation of germinal center formation / paraspeckles / negative regulation of B cell apoptotic process / regulation of immune system process / type 2 immune response / negative regulation of JNK cascade / positive regulation of regulatory T cell differentiation / nuclear thyroid hormone receptor binding / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / negative regulation of fatty acid metabolic process / negative regulation of glycolytic process / Notch-HLH transcription pathway / FOXO-mediated transcription of cell death genes / locomotor rhythm / histone deacetylase complex / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / regulation of cell differentiation / Differentiation of naive CD4+ T cells to T helper 2 cells (Th2 cells) / regulation of immune response / Regulation of MECP2 expression and activity / transcription repressor complex / Nuclear signaling by ERBB4 / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / spindle assembly / Regulation of lipid metabolism by PPARalpha / RORA,B,C and NR1D1 (REV-ERBA) regulate gene expression / Expression of BMAL (ARNTL), CLOCK, and NPAS2 / regulation of cytokine production / negative regulation of miRNA transcription / nuclear receptor binding / transcription corepressor binding / Heme signaling / HDACs deacetylate histones / PPARA activates gene expression / Cytoprotection by HMOX1 / Transcriptional activation of mitochondrial biogenesis / Downregulation of SMAD2/3:SMAD4 transcriptional activity / chromatin DNA binding / Transcriptional regulation of white adipocyte differentiation / negative regulation of cell growth / Nuclear Receptor transcription pathway / DNA-binding transcription repressor activity, RNA polymerase II-specific / mitotic spindle / NOTCH1 Intracellular Domain Regulates Transcription / Activation of anterior HOX genes in hindbrain development during early embryogenesis / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / histone deacetylase binding / HCMV Early Events / sequence-specific double-stranded DNA binding / transcription corepressor activity / regulation of cell population proliferation / regulation of inflammatory response / MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis / Interleukin-4 and Interleukin-13 signaling / DNA-binding transcription factor binding / sequence-specific DNA binding / RNA polymerase II-specific DNA-binding transcription factor binding / inflammatory response / transcription cis-regulatory region binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / positive regulation of apoptotic process / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / chromatin binding / DNA damage response / chromatin / negative regulation of transcription by RNA polymerase II / DNA-templated transcription / zinc ion binding / nucleoplasm / membrane / identical protein binding / nucleus / cytoplasm
Similarity search - Function
Nuclear receptor corepressor 1 N-terminal domain / N-CoR, GPS2-interacting domain / : / G-protein pathway suppressor 2-interacting domain / SANT domain profile. / SANT domain / Myb domain / Myb-like DNA-binding domain / BTB/POZ domain / BTB domain profile. ...Nuclear receptor corepressor 1 N-terminal domain / N-CoR, GPS2-interacting domain / : / G-protein pathway suppressor 2-interacting domain / SANT domain profile. / SANT domain / Myb domain / Myb-like DNA-binding domain / BTB/POZ domain / BTB domain profile. / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / Zinc finger, C2H2 type / SANT/Myb domain / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / zinc finger / Zinc finger C2H2 type domain profile. / Zinc finger C2H2 superfamily / Zinc finger C2H2 type domain signature. / SKP1/BTB/POZ domain superfamily / Zinc finger C2H2-type / Homeobox-like domain superfamily
Similarity search - Domain/homology
Nuclear receptor corepressor 1 / B-cell lymphoma 6 protein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.46 Å
AuthorsZacharchenko, T. / Wright, S.C.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Kay Kendall Leukaemia FundKKLF1047 United Kingdom
CitationJournal: Iucrj / Year: 2021
Title: Functionalization of the BCL6 BTB domain into a noncovalent crystallization chaperone.
Authors: Zacharchenko, T. / Wright, S.
History
DepositionJun 9, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 2, 2020Provider: repository / Type: Initial release
Revision 1.1Feb 3, 2021Group: Database references / Category: citation
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.2Mar 24, 2021Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.3Jan 24, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Nuclear receptor corepressor 1,B-cell lymphoma 6 protein
B: Nuclear receptor corepressor 1,B-cell lymphoma 6 protein
C: Nuclear receptor corepressor 1
D: Nuclear receptor corepressor 1
E: Nuclear receptor corepressor 1,B-cell lymphoma 6 protein
F: Nuclear receptor corepressor 1,B-cell lymphoma 6 protein
G: Nuclear receptor corepressor 1
I: Nuclear receptor corepressor 1,B-cell lymphoma 6 protein
J: Nuclear receptor corepressor 1,B-cell lymphoma 6 protein
K: Nuclear receptor corepressor 1
L: Nuclear receptor corepressor 1
H: Nuclear receptor corepressor 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)108,33044
Polymers105,27212
Non-polymers3,05832
Water2,162120
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area38660 Å2
ΔGint-552 kcal/mol
Surface area38980 Å2
MethodPISA
Unit cell
Length a, b, c (Å)198.575, 198.575, 113.067
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number92
Space group name H-MP41212
Space group name HallP4abw2nw
Symmetry operation#1: x,y,z
#2: -y+1/2,x+1/2,z+1/4
#3: y+1/2,-x+1/2,z+3/4
#4: x+1/2,-y+1/2,-z+3/4
#5: -x+1/2,y+1/2,-z+1/4
#6: -x,-y,z+1/2
#7: y,x,-z
#8: -y,-x,-z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
51
61
12
22
32
42
52
62

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111SERSERARGARG(chain 'A' and (resid -4 through 1 or resid 6...AA-4 - 14 - 9
121ASPASPILEILE(chain 'A' and (resid -4 through 1 or resid 6...AA6 - 3014 - 38
131THRTHRTHRTHR(chain 'A' and (resid -4 through 1 or resid 6...AA32 - 9240 - 100
141LEULEUPHEPHE(chain 'A' and (resid -4 through 1 or resid 6...AA95 - 124103 - 132
251SERSERARGARG(chain 'B' and (resid -4 through 1 or resid 6...BB-4 - 14 - 9
261ASPASPILEILE(chain 'B' and (resid -4 through 1 or resid 6...BB6 - 3014 - 38
271THRTHRTHRTHR(chain 'B' and (resid -4 through 1 or resid 6...BB32 - 9240 - 100
281LEULEUPHEPHE(chain 'B' and (resid -4 through 1 or resid 6...BB95 - 124103 - 132
391SERSERARGARG(chain 'E' and (resid -4 through 1 or resid 6...EE-4 - 14 - 9
3101ASPASPILEILE(chain 'E' and (resid -4 through 1 or resid 6...EE6 - 3014 - 38
3111THRTHRTHRTHR(chain 'E' and (resid -4 through 1 or resid 6...EE32 - 9240 - 100
3121LEULEUPHEPHE(chain 'E' and (resid -4 through 1 or resid 6...EE95 - 124103 - 132
4131SERSERARGARG(chain 'F' and (resid -4 through 1 or resid 6...FF-4 - 14 - 9
4141ASPASPILEILE(chain 'F' and (resid -4 through 1 or resid 6...FF6 - 3014 - 38
4151THRTHRTHRTHR(chain 'F' and (resid -4 through 1 or resid 6...FF32 - 9240 - 100
4161LEULEUPHEPHE(chain 'F' and (resid -4 through 1 or resid 6...FF95 - 124103 - 132
5171SERSERARGARG(chain 'I' and (resid -4 through 1 or resid 6...IH-4 - 14 - 9
5181ASPASPILEILE(chain 'I' and (resid -4 through 1 or resid 6...IH6 - 3014 - 38
5191THRTHRTHRTHR(chain 'I' and (resid -4 through 1 or resid 6...IH32 - 9240 - 100
5201LEULEUPHEPHE(chain 'I' and (resid -4 through 1 or resid 6...IH95 - 124103 - 132
6211SERSERARGARG(chain 'J' and (resid -4 through 1 or resid 6...JI-4 - 14 - 9
6221ASPASPILEILE(chain 'J' and (resid -4 through 1 or resid 6...JI6 - 3014 - 38
6231THRTHRTHRTHR(chain 'J' and (resid -4 through 1 or resid 6...JI32 - 9240 - 100
6241LEULEUPHEPHE(chain 'J' and (resid -4 through 1 or resid 6...JI95 - 124103 - 132
1252THRTHRARGARGchain 'C'CC1342 - 13563 - 17
2262THRTHRARGARG(chain 'D' and resid 1342 through 1356)DD1342 - 13563 - 17
3272THRTHRARGARGchain 'G'GG1342 - 13563 - 17
4282THRTHRARGARG(chain 'H' and resid 1342 through 1356)HL1342 - 13563 - 17
5292THRTHRARGARGchain 'K'KJ1342 - 13563 - 17
6302THRTHRARGARG(chain 'L' and resid 1342 through 1356)LK1342 - 13563 - 17

NCS ensembles :
ID
1
2

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Components

#1: Protein
Nuclear receptor corepressor 1,B-cell lymphoma 6 protein / N-CoR1 / BCL-6 / B-cell lymphoma 5 protein / BCL-5 / Protein LAZ-3 / Zinc finger and BTB domain- ...N-CoR1 / BCL-6 / B-cell lymphoma 5 protein / BCL-5 / Protein LAZ-3 / Zinc finger and BTB domain-containing protein 27 / Zinc finger protein 51


Mass: 15602.981 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NCOR1, KIAA1047, BCL6, BCL5, LAZ3, ZBTB27, ZNF51 / Production host: Escherichia coli (E. coli) / References: UniProt: O75376, UniProt: P41182
#2: Protein/peptide
Nuclear receptor corepressor 1 / N-CoR1


Mass: 1942.288 Da / Num. of mol.: 6 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: O75376
#3: Chemical...
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 28 / Source method: obtained synthetically / Formula: SO4
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 120 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 5.29 Å3/Da
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: 1.34M Ammonium sulfate 0.67%(v/v) MPD 0.1M HEPES/ Sodium hydroxide pH 7.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID30B / Wavelength: 0.966 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jul 27, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.966 Å / Relative weight: 1
ReflectionResolution: 2.46→98.32 Å / Num. obs: 82349 / % possible obs: 99.9 % / Redundancy: 5.8 % / Biso Wilson estimate: 48.21 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.15 / Net I/σ(I): 6.9
Reflection shellResolution: 2.46→2.52 Å / Redundancy: 5.9 % / Num. unique obs: 6006 / CC1/2: 0.345 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
PHENIX1.17.1_3660refinement
XDSdata reduction
XDSdata scaling
REFMACphasing
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6XYX

6xyx


Resolution: 2.46→88.81 Å / SU ML: 0.3787 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 31.3545
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2593 4013 4.89 %
Rwork0.2243 78060 -
obs0.226 82073 99.61 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 70.33 Å2
Refinement stepCycle: LAST / Resolution: 2.46→88.81 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7112 0 164 120 7396
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.017371
X-RAY DIFFRACTIONf_angle_d1.15819941
X-RAY DIFFRACTIONf_chiral_restr0.06491140
X-RAY DIFFRACTIONf_plane_restr0.00731248
X-RAY DIFFRACTIONf_dihedral_angle_d19.32442769
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.46-2.490.37471300.3812630X-RAY DIFFRACTION98.92
2.49-2.520.42331240.36762676X-RAY DIFFRACTION99.86
2.52-2.550.37121320.36252659X-RAY DIFFRACTION100
2.55-2.580.38711450.34692641X-RAY DIFFRACTION99.75
2.58-2.620.40551430.34722667X-RAY DIFFRACTION99.93
2.62-2.650.3691400.34032652X-RAY DIFFRACTION99.71
2.65-2.690.3471420.32132675X-RAY DIFFRACTION99.68
2.69-2.740.31181300.31572649X-RAY DIFFRACTION99.96
2.74-2.780.27321320.29362683X-RAY DIFFRACTION100
2.78-2.830.34011470.29052684X-RAY DIFFRACTION99.86
2.83-2.880.35841420.28972652X-RAY DIFFRACTION99.86
2.88-2.940.33371330.26312676X-RAY DIFFRACTION99.61
2.94-30.30811470.25412658X-RAY DIFFRACTION99.75
3-3.060.28931280.24832697X-RAY DIFFRACTION99.72
3.06-3.130.2651490.23482655X-RAY DIFFRACTION99.82
3.13-3.210.25481330.24012684X-RAY DIFFRACTION99.93
3.21-3.30.27181250.24112692X-RAY DIFFRACTION99.96
3.3-3.390.26391250.23742702X-RAY DIFFRACTION99.86
3.39-3.50.26371430.2262688X-RAY DIFFRACTION99.72
3.5-3.630.23441290.23732718X-RAY DIFFRACTION99.72
3.63-3.770.33361490.25662684X-RAY DIFFRACTION99.86
3.77-3.950.2371450.20072692X-RAY DIFFRACTION99.96
3.95-4.150.21521310.18342730X-RAY DIFFRACTION99.62
4.15-4.410.18921500.1612689X-RAY DIFFRACTION99.96
4.41-4.760.24381350.16362749X-RAY DIFFRACTION100
4.76-5.230.1761390.16132733X-RAY DIFFRACTION99.62
5.23-5.990.23611500.19542758X-RAY DIFFRACTION99.9
5.99-7.550.22991480.20242797X-RAY DIFFRACTION99.93
7.55-88.810.18511470.17012790X-RAY DIFFRACTION94.65
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.807020620821.07745288725-2.081807439841.62460250228-1.884002072733.999907447110.0601782782448-0.0659927822838-0.07603659441590.366379608270.01171933333150.146369057647-0.0529374659143-0.101026705095-0.04890852959460.504691636452-0.05687909579990.003592138013150.4831217665940.0261985500160.2270006460797.4044817330485.2172034772-50.1398670816
20.9910589963370.889964372592-1.391666416471.86616081771-1.594521718884.03003132015-0.08511976384260.2135416044360.0232571772394-0.09651959184340.0647155537819-0.072280987416-0.2104390146860.03223879514150.01017618376550.475956849167-0.03383772880370.03840666135860.5038555146950.005630219082210.21553766916213.836311089790.8737520026-64.1509637503
36.85217145945-3.589581982924.771287924888.356767677-4.416595912128.18693437595-0.131195225920.214608552244-0.893007411853-0.1024257751070.2036566769570.05092416062840.95873249036-0.009719649627090.1105665353520.60364610326-0.02287926257290.08276650902040.6061538950650.02336055675720.29534742052810.471814515474.8942199347-52.989554651
48.10374438495-3.75383030992-3.650644454866.585793559353.493816795648.748162505250.1263504804280.298721098337-0.3661726130760.286153969362-0.265361060664-0.3762665293320.08884849675050.8402499330620.2646724580340.517321839006-0.016034999604-0.001726845166550.7278447501560.06896710806080.36026010885423.842314414688.0466977479-61.5560278916
52.02882781021.40490778001-0.458173839933.52323790504-1.309649092910.996105513772-0.1102515047390.3298452231170.0626910659352-0.3051414936610.3716996331610.174442125143-0.0699474163956-0.434916721679-0.2474499568810.591925946619-0.1008706092870.04565338419110.6120359933660.0326277194950.258732590044-8.6089808281257.7569988916-30.9435311285
61.915037126261.49359218622-0.6108861992574.66868066949-0.878511677281.8107179904-0.2322732189460.32389195966-0.34247268395-0.2076430866980.4420927322330.02693953475290.53654431182-0.846010410228-0.2030040034440.676624908506-0.321398756423-0.003578573781950.781333379358-0.003330881091680.383577708351-14.84326363541.9757046487-29.7415799419
75.88104996111-6.331175964012.911295635139.22688327609-3.344503934715.08963609075-0.1247804569541.79544819045-0.131766539952-1.03705402534-0.3301455568250.2445916585770.483738092519-0.2006576752130.307932571220.841102998578-0.328983419707-0.03225058084110.8401801065340.045486216610.410530519893-10.197177613556.812885267-42.2077006735
81.6550548881-1.1393818154-0.5029649423343.681325707510.9068818321491.57577262027-0.0896359920607-0.3217697917170.007241217131360.3694332420060.36966222911-0.1966723397570.1756970393850.662889380342-0.2319450154130.6289861768430.1431162871480.0168362115730.630732999266-0.04653378616620.2676661352638.4006328924658.20600103742.14084633955
91.61808408886-1.68041999522-0.5712428709894.412389549380.7171810132592.38261057907-0.263148932103-0.250988641436-0.332376063170.1842875669520.5247207580390.02466024340250.9270306083830.845355038623-0.2689941175340.9973862381950.415788584414-0.02948240557160.868221073564-0.03086447822530.43643436313214.883145370943.32668194961.2314884854
105.183481847944.616020326114.348600861154.451150241954.128717186033.844709205050.0265138042906-1.13186128429-0.2312162313280.7768496903570.0273090250464-0.4533647192370.628893762888-0.0943653764821-0.02393192520690.8933954718260.337857227242-0.02515263430.925573733379-0.08391319963010.49090982367810.141122152857.536963902113.3388249323
112.848477554611.72249341-2.191806389333.16605989899-2.422361803088.499353352080.143626140623-0.390141941996-0.1734766089960.6795364635290.0722495203832-0.8709827372150.4360355787581.49456831683-0.373850461091.09726213990.671064361344-0.115746334381.46026014889-0.2469749774520.60157069852524.723360191945.44094971955.9334838161
127.11101163547-0.663368209126-3.893219719225.312546307093.470370525874.115042349970.07270369924080.6910979003360.407944960014-0.480360298367-0.2157677461980.6912446801310.384734576001-1.78469549339-0.02534753569060.833617428444-0.456656704429-0.08157002241051.139948707970.3071603601910.477652848203-24.44323344.3348554042-34.687800861
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain 'A' and resid -7 through 129)
2X-RAY DIFFRACTION2(chain 'B' and resid -7 through 129)
3X-RAY DIFFRACTION3(chain 'C' and resid 1342 through 1356)
4X-RAY DIFFRACTION4(chain 'D' and resid 1341 through 1356)
5X-RAY DIFFRACTION5(chain 'E' and resid -4 through 127)
6X-RAY DIFFRACTION6(chain 'F' and resid -5 through 125)
7X-RAY DIFFRACTION7(chain 'G' and resid 1342 through 1356)
8X-RAY DIFFRACTION8(chain 'I' and resid -4 through 127)
9X-RAY DIFFRACTION9(chain 'J' and resid -7 through 124)
10X-RAY DIFFRACTION10(chain 'K' and resid 1342 through 1356)
11X-RAY DIFFRACTION11(chain 'L' and resid 1341 through 1356)
12X-RAY DIFFRACTION12(chain 'H' and resid 1341 through 1356)

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