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Open data
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Basic information
Entry | Database: PDB / ID: 6zb9 | ||||||
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Title | Exo-beta-1,3-glucanase from moose rumen microbiome, wild type | ||||||
![]() | Exo-beta-1,3-glucanase | ||||||
![]() | HYDROLASE / Glycoside hydrolase / exo-beta-1 / 3-glucanase / family GH5_44 | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kalyani, D.C. / Reichenbach, T. / Aspeborg, H. / Divne, C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A homodimeric bacterial exo-beta-1,3-glucanase derived from moose rumen microbiome shows a structural framework similar to yeast exo-beta-1,3-glucanases. Authors: Kalyani, D.C. / Reichenbach, T. / Aspeborg, H. / Divne, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 159 KB | Display | ![]() |
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PDB format | ![]() | 126.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.8 KB | Display | ![]() |
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Full document | ![]() | 436.4 KB | Display | |
Data in XML | ![]() | 25.9 KB | Display | |
Data in CIF | ![]() | 35.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 45381.680 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: An active-site loop is disordered in the model and has therefore not been modeled. Source: (gene. exp.) ![]() Plasmid: pET-11a / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.5 / Details: 0.1 M Tris-HCl, 0.2 M MgCl2, 30% PEG 4000 |
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-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 26, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.072897 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→47.55 Å / Num. obs: 44159 / % possible obs: 96.8 % / Redundancy: 3.4 % / CC1/2: 0.983 / Net I/σ(I): 5.8 |
Reflection shell | Resolution: 2.5→2.6 Å / Num. unique obs: 4164 / CC1/2: 0.359 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: SAD-generated model Resolution: 2.5→47.547 Å / SU ML: 0.41 / Cross valid method: FREE R-VALUE / σ(F): 1.08 / Phase error: 31.3 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→47.547 Å
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Refine LS restraints |
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LS refinement shell |
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