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TitleA homodimeric bacterial exo-beta-1,3-glucanase derived from moose rumen microbiome shows a structural framework similar to yeast exo-beta-1,3-glucanases.
Journal, issue, pagesEnzyme. Microb. Technol., Vol. 143, Page 109723-109723, Year 2021
Publish dateJun 8, 2020 (structure data deposition date)
AuthorsKalyani, D.C. / Reichenbach, T. / Aspeborg, H. / Divne, C.
External linksEnzyme. Microb. Technol. / PubMed:33375982
MethodsX-ray diffraction
Resolution1.35 - 2.5 Å
Structure data

PDB-6zb8:
Exo-beta-1,3-glucanase from moose rumen microbiome, active site mutant E167Q/E295Q
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-6zb9:
Exo-beta-1,3-glucanase from moose rumen microbiome, wild type
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-15P:
POLYETHYLENE GLYCOL (N=34) / precipitant*YM

ChemComp-HOH:
WATER

Source
  • uncultured bacterium (environmental samples)
KeywordsHYDROLASE / Glycoside hydrolase / Exo-beta-1 / 3-glucanase / family GH5_44 / active-site mutant

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