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- PDB-6ybo: RT structure of Glucose Isomerase obtained at 1.06 A resolution f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ybo | ||||||
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Title | RT structure of Glucose Isomerase obtained at 1.06 A resolution from crystal grown in a Kapton microchip. | ||||||
![]() | Xylose isomerase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gavira, J.A. / Martinez-Rodriguez, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips. Authors: Gavira, J.A. / Rodriguez-Ruiz, I. / Martinez-Rodriguez, S. / Basu, S. / Teychene, S. / McCarthy, A.A. / Mueller-Dieckman, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 228.8 KB | Display | ![]() |
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PDB format | ![]() | 154.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6ybfC ![]() 6ybiC ![]() 6ybrC ![]() 6ybxC ![]() 6yc5C ![]() 4a8iS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | ![]() Mass: 43283.297 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | ChemComp-NA / | #4: Water | ChemComp-HOH / | ![]() Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54 % |
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Crystal grow![]() | Temperature: 293.5 K / Method: counter-diffusion / pH: 7 / Details: 0.1 M MgCl2, 10% w/v PEG 1K, 0.1 M Hepes pH 7.0 / Temp details: Room temperature |
-Data collection
Diffraction | Mean temperature: 293.5 K / Ambient temp details: Room Temperature / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 6, 2017 Details: Be CRL lenses for vertical focusing and Rh/Pt/Si coated ellipitcal mirror for horizontal focusing |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.06→41.12 Å / Num. obs: 200000 / % possible obs: 92.32 % / Redundancy: 7.93 % / Biso Wilson estimate: 13.05 Å2 / CC1/2: 0.987 / Net I/σ(I): 8.4 |
Reflection shell | Resolution: 1.06→1.09 Å / Num. unique obs: 8688 / CC1/2: 0.614 / % possible all: 40.2 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4A8I Resolution: 1.06→41.12 Å / SU ML: 0.091 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 13.0028
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.18 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.06→41.12 Å
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Refine LS restraints |
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LS refinement shell |
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