[English] 日本語
![](img/lk-miru.gif)
- PDB-6y9n: Crystal structure of Whirlin PDZ3_C-ter in complex with Myosin 15... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 6y9n | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of Whirlin PDZ3_C-ter in complex with Myosin 15a C-terminal PDZ binding motif peptide | ||||||
![]() |
| ||||||
![]() | STRUCTURAL PROTEIN / Whirlin / PDZ / Myosin 15a / complex | ||||||
Function / homology | ![]() axon collateral / paranodal junction maintenance / periciliary membrane compartment / USH2 complex / stereocilia ankle link / actin-based cell projection / inner ear receptor cell differentiation / stereocilia ankle link complex / sensory perception of light stimulus / cerebellar Purkinje cell layer formation ...axon collateral / paranodal junction maintenance / periciliary membrane compartment / USH2 complex / stereocilia ankle link / actin-based cell projection / inner ear receptor cell differentiation / stereocilia ankle link complex / sensory perception of light stimulus / cerebellar Purkinje cell layer formation / photoreceptor connecting cilium / stereocilium tip / inner ear receptor cell stereocilium organization / detection of mechanical stimulus involved in sensory perception of sound / stereocilium bundle / stereocilium / apical dendrite / retina homeostasis / myosin complex / auditory receptor cell stereocilium organization / inner ear morphogenesis / microfilament motor activity / response to light stimulus / photoreceptor inner segment / ciliary basal body / dendritic shaft / locomotory behavior / establishment of localization in cell / actin filament / actin filament organization / sensory perception of sound / establishment of protein localization / cilium / endocytosis / actin filament binding / actin cytoskeleton / presynapse / growth cone / postsynapse / neuron projection / protein domain specific binding / neuronal cell body / positive regulation of gene expression / perinuclear region of cytoplasm / ATP binding / identical protein binding / membrane / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhu, Y. / Delhommel, F. / Haouz, A. / Caillet-Saguy, C. / Vaney, M. / Mechaly, A.E. / Wolff, N. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Deciphering the Unexpected Binding Capacity of the Third PDZ Domain of Whirlin to Various Cochlear Hair Cell Partners. Authors: Zhu, Y. / Delhommel, F. / Cordier, F. / Luchow, S. / Mechaly, A. / Colcombet-Cazenave, B. / Girault, V. / Pepermans, E. / Bahloul, A. / Gautier, C. / Brule, S. / Raynal, B. / Hoos, S. / ...Authors: Zhu, Y. / Delhommel, F. / Cordier, F. / Luchow, S. / Mechaly, A. / Colcombet-Cazenave, B. / Girault, V. / Pepermans, E. / Bahloul, A. / Gautier, C. / Brule, S. / Raynal, B. / Hoos, S. / Haouz, A. / Caillet-Saguy, C. / Ivarsson, Y. / Wolff, N. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 59.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 40.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 430.8 KB | Display | |
Data in XML | ![]() | 7 KB | Display | |
Data in CIF | ![]() | 8.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6y38C ![]() 6y9oC ![]() 6y9pC ![]() 6y9qC ![]() 1ufxS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 11359.070 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() References: UniProt: Q80VW5 |
---|---|
#2: Protein/peptide | Mass: 1482.741 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.8 Å3/Da / Density % sol: 67.6 % |
---|---|
Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, sitting drop Details: 0.1M HEPES pH 7.5,10% v/v isopropanol,20% w/v PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 300K / Detector: PIXEL / Date: Sep 29, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9786 Å / Relative weight: 1 |
Reflection | Resolution: 1.93→15.7 Å / Num. obs: 14149 / % possible obs: 99.38 % / Redundancy: 7.2 % / CC1/2: 0.998 / CC star: 1 / Net I/σ(I): 13.01 |
Reflection shell | Resolution: 1.93→1.999 Å / Num. unique obs: 1403 / CC1/2: 0.659 / CC star: 0.891 |
-
Processing
Software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1UFX Resolution: 1.93→15.7 Å / Cross valid method: FREE R-VALUE
| ||||||||||||||||||||||||
Displacement parameters | Biso mean: 48.37 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.93→15.7 Å
| ||||||||||||||||||||||||
Refine LS restraints |
|