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- PDB-6xzw: Crystal structure of the meningococcal vaccine antigen fHbp in co... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6xzw | ||||||
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Title | Crystal structure of the meningococcal vaccine antigen fHbp in complex with a cross-reactive human Fab. | ||||||
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![]() | IMMUNE SYSTEM / Antigen-antibody complex / epitope mapping / lipoprotein / neisseria meningitidis | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Veggi, D. / Cozzi, R. | ||||||
![]() | ![]() Title: 4CMenB vaccine induces elite cross-protective human antibodies that compete with human factor H for binding to meningococcal fHbp. Authors: Veggi, D. / Bianchi, F. / Santini, L. / Lo Surdo, P. / Chesterman, C.C. / Pansegrau, W. / Bechi, N. / Huang, Y. / Masignani, V. / Pizza, M. / Rappuoli, R. / Bottomley, M.J. / Cozzi, R. / Maione, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 154.7 KB | Display | ![]() |
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PDB format | ![]() | 109.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.4 KB | Display | ![]() |
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Full document | ![]() | 459.1 KB | Display | |
Data in XML | ![]() | 25.8 KB | Display | |
Data in CIF | ![]() | 35.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 26896.045 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: NMB1870 / Production host: ![]() ![]() | ||||
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#2: Antibody | Mass: 23787.438 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() | ||||
#3: Antibody | Mass: 23642.670 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() | ||||
#4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.85 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / Details: 0.1M HEPES, 20% w/v jeff ED-2001, pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Jul 17, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.966 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→46.78 Å / Num. obs: 29802 / % possible obs: 93 % / Redundancy: 3.4 % / Biso Wilson estimate: 50.17 Å2 / CC1/2: 0.99 / Net I/σ(I): 16.5 |
Reflection shell | Resolution: 2.4→2.48 Å / Num. unique obs: 3202 / CC1/2: 0.82 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3KVD, 5TLK Resolution: 2.4→46.78 Å / SU ML: 0.31 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.48
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||
Displacement parameters | Biso mean: 55.68 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→46.78 Å
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Refine LS restraints | Type: f_chiral_restr / Dev ideal: 0.0735652181087 / Number: 779 | ||||||||||||||||||
LS refinement shell | Resolution: 2.4→2.48 Å
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