- PDB-6xzc: CryoEM structure of the ring-shaped virulence factor EspB from My... -
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基本情報
登録情報
データベース: PDB / ID: 6xzc
タイトル
CryoEM structure of the ring-shaped virulence factor EspB from Mycobacterium tuberculosis
要素
ESX-1 secretion-associated protein EspB
キーワード
TRANSPORT PROTEIN / M. tuberculosis / ESX-1 / Type VII secretion system / EspB / Rv3881c / EsxA / CFP10 / ESAT-6
機能・相同性
ESX-1 secretion-associated protein EspB, PE domain / ESX-1 secreted protein B PE domain / protein secretion by the type VII secretion system / PPE superfamily / biological process involved in interaction with host / extracellular region / identical protein binding / ESX-1 secretion-associated protein EspB
ジャーナル: J Struct Biol X / 年: 2020 タイトル: High resolution CryoEM structure of the ring-shaped virulence factor EspB from . 著者: Jérémie Piton / Florence Pojer / Soichi Wakatsuki / Cornelius Gati / Stewart T Cole / 要旨: The EspB protein of is a 60 kDa virulence factor, implicated in conjugation and exported by the ESX-1 system of which it may also be a component. Previous attempts to obtain high-resolution maps of ...The EspB protein of is a 60 kDa virulence factor, implicated in conjugation and exported by the ESX-1 system of which it may also be a component. Previous attempts to obtain high-resolution maps of EspB by cryo-electron microscopic examination of single particles have been thwarted by severe orientation bias of the particles. This was overcome by using detergent as a surfactant thereby allowing reconstruction of the EspB structure at 3.37 Å resolution. The final structure revealed the N-terminal domain of EspB to be organized as a cylindrical heptamer with dimensions of 90 Å x 90 Å and a central channel of 45 Å diameter whereas the C-terminal domain was unstructured. New atomic insight was obtained into the helical packing required for protomer interactions and the overall electrostatic potential. The external surface is electronegatively charged while the channel is lined with electropositive patches. EspB thus has many features of a pore-like transport protein that might allow the passage of an ESX-1 substrate such as the 35 Å diameter EsxA-EsxB heterodimer or B-form DNA consistent with its proposed role in DNA uptake.
A: ESX-1 secretion-associated protein EspB B: ESX-1 secretion-associated protein EspB C: ESX-1 secretion-associated protein EspB D: ESX-1 secretion-associated protein EspB E: ESX-1 secretion-associated protein EspB F: ESX-1 secretion-associated protein EspB G: ESX-1 secretion-associated protein EspB
装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE 詳細: 0.05% fluorinated octylmaltoside was add into protein solution and immediately applied to a freshly glow-discharged holey carbon grid. ). Excess liquid was blotted for 2.3 s using an FEI ...詳細: 0.05% fluorinated octylmaltoside was add into protein solution and immediately applied to a freshly glow-discharged holey carbon grid. ). Excess liquid was blotted for 2.3 s using an FEI Vitrobot Mark IV and the sample was plunge frozen in liquid ethane.