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- PDB-6x5t: Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A3-Asn -

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Open data


ID or keywords:

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Basic information

Entry
Database: PDB / ID: 6x5t
TitleHuman Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A3-Asn
ComponentsAlpha-(1,6)-fucosyltransferase
KeywordsTRANSFERASE / Glycosyl transferase / Fucosyl transferase / GT-B fold / Inverting
Function / homology
Function and homology information


glycoprotein 6-alpha-L-fucosyltransferase / glycoprotein 6-alpha-L-fucosyltransferase activity / receptor metabolic process / GDP-L-fucose metabolic process / alpha-(1->6)-fucosyltransferase activity / : / Reactions specific to the complex N-glycan synthesis pathway / oligosaccharide biosynthetic process / L-fucose catabolic process / N-glycan processing ...glycoprotein 6-alpha-L-fucosyltransferase / glycoprotein 6-alpha-L-fucosyltransferase activity / receptor metabolic process / GDP-L-fucose metabolic process / alpha-(1->6)-fucosyltransferase activity / : / Reactions specific to the complex N-glycan synthesis pathway / oligosaccharide biosynthetic process / L-fucose catabolic process / N-glycan processing / regulation of cellular response to oxidative stress / protein N-linked glycosylation via asparagine / respiratory gaseous exchange by respiratory system / fibroblast migration / protein N-linked glycosylation / Golgi cisterna membrane / transforming growth factor beta receptor signaling pathway / integrin-mediated signaling pathway / SH3 domain binding / regulation of gene expression / Maturation of spike protein / in utero embryonic development / viral protein processing / Golgi membrane / Golgi apparatus / extracellular exosome / membrane
Similarity search - Function
Alpha-(1,6)-fucosyltransferase / Alpha-(1,6)-fucosyltransferase, SH3 domain / Alpha-(1,6)-fucosyltransferase, N- and catalytic domain / Alpha-(1,6)-fucosyltransferase N- and catalytic domains / Glycosyltransferase family 23 (GT23) domain / Glycosyltransferase family 23 (GT23) domain profile. / Variant SH3 domain / Src homology 3 domains / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / Alpha-(1,6)-fucosyltransferase
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.47 Å
AuthorsKadirvelraj, R. / Wood, Z.A.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism (NIH/NIAAA)R01GM130915, P41GM103390 and P01GM107012 United States
CitationJournal: J.Biol.Chem. / Year: 2020
Title: Characterizing human alpha-1,6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases.
Authors: Boruah, B.M. / Kadirvelraj, R. / Liu, L. / Ramiah, A. / Li, C. / Zong, G. / Bosman, G.P. / Yang, J.Y. / Wang, L.X. / Boons, G.J. / Wood, Z.A. / Moremen, K.W.
History
DepositionMay 26, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 7, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 21, 2020Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Dec 23, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4Oct 30, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Alpha-(1,6)-fucosyltransferase
B: Alpha-(1,6)-fucosyltransferase
C: Alpha-(1,6)-fucosyltransferase
D: Alpha-(1,6)-fucosyltransferase
E: Alpha-(1,6)-fucosyltransferase
F: Alpha-(1,6)-fucosyltransferase
G: Alpha-(1,6)-fucosyltransferase
H: Alpha-(1,6)-fucosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)502,29922
Polymers494,7628
Non-polymers7,53714
Water13,619756
1
A: Alpha-(1,6)-fucosyltransferase
B: Alpha-(1,6)-fucosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,6216
Polymers123,6902
Non-polymers1,9304
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10210 Å2
ΔGint-25 kcal/mol
Surface area38600 Å2
MethodPISA
2
C: Alpha-(1,6)-fucosyltransferase
D: Alpha-(1,6)-fucosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,5295
Polymers123,6902
Non-polymers1,8383
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10010 Å2
ΔGint-22 kcal/mol
Surface area38620 Å2
MethodPISA
3
E: Alpha-(1,6)-fucosyltransferase
F: Alpha-(1,6)-fucosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,6216
Polymers123,6902
Non-polymers1,9304
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10060 Å2
ΔGint-25 kcal/mol
Surface area38560 Å2
MethodPISA
4
G: Alpha-(1,6)-fucosyltransferase
H: Alpha-(1,6)-fucosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,5295
Polymers123,6902
Non-polymers1,8383
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9690 Å2
ΔGint-22 kcal/mol
Surface area38710 Å2
MethodPISA
Unit cell
Length a, b, c (Å)151.690, 151.690, 474.070
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number170
Space group name H-MP65

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Components

#1: Protein
Alpha-(1,6)-fucosyltransferase / Alpha1-6FucT / Fucosyltransferase 8 / GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1 / 6- ...Alpha1-6FucT / Fucosyltransferase 8 / GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1 / 6-fucosyltransferase / GDP-fucose--glycoprotein fucosyltransferase / Glycoprotein 6-alpha-L-fucosyltransferase


Mass: 61845.219 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FUT8 / Cell (production host): HEK293 / Production host: Homo sapiens (human)
References: UniProt: Q9BYC5, glycoprotein 6-alpha-L-fucosyltransferase
#2: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 951.875 Da / Num. of mol.: 4 / Source method: isolated from a natural source
DescriptorTypeProgram
DGlcpNAcb1-2DManpa1-3DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-1/a4-b1_b4-c1_c3-d1_d2-e1WURCSPDB2Glycan 1.1.0
[][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][b-D-GlcpNAc]{}}}}}LINUCSPDB-CARE
#3: Chemical
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: GDP, energy-carrying molecule*YM
#4: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 756 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY
Has protein modificationY
Nonpolymer detailsA3-Asn was purified as a glycosylated peptide and proteolyzed to an asparagine-linked ...A3-Asn was purified as a glycosylated peptide and proteolyzed to an asparagine-linked oligosaccharide. The asparagine is not visible in the electron density
Source detailsA3-Asn was purified from egg yolk powder

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.3 Å3/Da / Density % sol: 62 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 / Details: 10% PEG 3350, 0.2 M L-proline, 100 mM Hepes pH 7.2 / Temp details: Room temperature

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 1, 2020
RadiationMonochromator: Si (111) Rosenbaum-Rock double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.47→87.998 Å / Num. obs: 219502 / % possible obs: 99.8 % / Redundancy: 5.1 % / Biso Wilson estimate: 54.6 Å2 / CC1/2: 0.997 / Rrim(I) all: 0.12 / Net I/σ(I): 10.7
Reflection shellResolution: 2.47→2.53 Å / Redundancy: 5.3 % / Mean I/σ(I) obs: 1.4 / Num. unique obs: 16316 / CC1/2: 0.505 / Rrim(I) all: 1.05 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX1.13_2998refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6X5H

6x5h


Resolution: 2.47→87.998 Å / Cross valid method: FREE R-VALUE / σ(F): 2.05 / Phase error: 29.98 / Stereochemistry target values: TWIN_LSQ_F
RfactorNum. reflection% reflection
Rfree0.2003 10983 5.01 %
Rwork0.1655 --
obs0.1684 219384 99.85 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.47→87.998 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms30384 0 496 757 31637
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00231742
X-RAY DIFFRACTIONf_angle_d0.53443133
X-RAY DIFFRACTIONf_dihedral_angle_d17.33618925
X-RAY DIFFRACTIONf_chiral_restr0.044614
X-RAY DIFFRACTIONf_plane_restr0.0035489
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.4702-2.51280.34485630.290210462X-RAY DIFFRACTION95
2.5128-2.55850.32375530.28510404X-RAY DIFFRACTION95
2.5585-2.60770.31525530.27510440X-RAY DIFFRACTION95
2.6077-2.66090.30365520.255210438X-RAY DIFFRACTION95
2.6609-2.71880.2775470.249110349X-RAY DIFFRACTION95
2.7188-2.7820.29745540.246610402X-RAY DIFFRACTION95
2.782-2.85160.26885450.228810461X-RAY DIFFRACTION95
2.8516-2.92870.25325500.219210438X-RAY DIFFRACTION95
2.9287-3.01490.23145470.20310439X-RAY DIFFRACTION95
3.0149-3.11220.24395440.193910393X-RAY DIFFRACTION95
3.1122-3.22340.24955500.193210430X-RAY DIFFRACTION95
3.2234-3.35250.2265470.184610425X-RAY DIFFRACTION95
3.3525-3.50510.2025360.176710438X-RAY DIFFRACTION95
3.5051-3.68990.20275500.166910425X-RAY DIFFRACTION95
3.6899-3.92110.18715460.157810342X-RAY DIFFRACTION94
3.9211-4.22380.16645450.140410346X-RAY DIFFRACTION94
4.2238-4.64880.15385460.122810402X-RAY DIFFRACTION95
4.6488-5.32150.15535490.120410440X-RAY DIFFRACTION95
5.3215-6.70410.19155490.142110451X-RAY DIFFRACTION95
6.7041-87.9980.15045440.134510471X-RAY DIFFRACTION95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.87643.86191.99695.02111.12021.83210.16590.34170.45060.4513-0.05290.12210.32410.2047-0.2270.41190.05450.01430.40490.05570.3175136.3303150.933165.7727
23.51750.79040.43872.78590.65442.49250.19470.17550.36090.19-0.1432-0.19840.12160.2101-0.07740.3708-0.0054-0.02620.38030.08940.3027142.0864158.955758.9281
31.592-0.79640.56722.6458-0.2820.8069-0.1037-0.0435-0.17490.04880.25270.58-0.0201-0.3454-0.1530.32820.05220.0180.47150.07470.3645105.1769170.776341.4
42.2068-0.4339-1.00491.6673-0.24721.13820.06640.1271-0.0154-0.087-0.02210.2567-0.4685-0.4166-0.11530.4670.0783-0.06820.50920.11950.3531104.0439181.642132.0215
51.7151-0.95850.12762.67210.59660.9225-0.0625-0.3552-0.01790.20770.04950.4528-0.1639-0.26910.00470.46950.10040.08550.66510.0840.4093101.2874177.810250.4322
62.3109-0.21861.73290.7963-0.7311.7526-0.0181-0.01630.0124-0.02810.02110.0491-0.19320.08930.00010.35520.00830.01290.30650.04380.2672122.4822172.903336.8663
71.658-0.81951.28511.5649-0.47881.9975-0.08650.1160.1501-0.06970.116-0.1451-0.03790.084-0.04980.3604-0.03350.00770.4130.05210.2495134.1084172.516637.3107
81.1487-0.22390.75520.8824-0.43711.4348-0.1078-0.20390.07040.2550.06170.1304-0.3064-0.09980.06620.470.0810.04880.41420.03840.2597119.7559172.336858.8035
92.20651.151-0.76832.9943-0.93170.72380.22040.10660.13860.5951-0.0324-0.0514-0.15850.008-0.24240.42440.0578-0.00230.3390.03710.2346132.3268162.338867.2935
101.3055-0.64580.19391.4049-0.28970.7560.021-0.019-0.1885-0.01850.00090.12430.0513-0.0452-0.04590.3192-0.02940.01550.3360.03750.3184131.7244117.783174.4563
110.47390.06610.17960.9566-0.09450.67230.0332-0.0275-0.05160.0651-0.01780.0035-0.1285-0.0766-0.00590.3370.02070.02820.36910.03430.2518137.3656139.121979.4367
120.7238-1.0205-0.12941.93350.68871.01540.03940.1809-0.03860.37930.0718-0.05370.28170.1416-0.11790.3026-0.02510.0480.37810.00260.2239177.767797.448669.6188
131.1301-0.4153-0.03991.56660.29231.1720.0074-0.00920.190.02480.0594-0.1455-0.0834-0.0114-0.080.3375-0.02310.00710.2945-0.01080.2919168.4946129.670485.1633
140.4983-0.2178-0.02910.7316-0.07010.53950.0070.08410.05940.0028-0.0040.04240.0218-0.03030.02010.3233-0.0183-0.00150.3474-0.00980.2485164.3091107.684678.7481
152.1763-0.2623-1.40151.3588-0.64261.46820.07850.2241-0.0958-0.05480.01330.1660.0264-0.1278-0.12770.29840.0033-0.02460.4084-0.06520.2557169.72296.781160.0894
164.0814-0.5435-3.10145.39062.83.49170.2674-0.1910.945-0.33940.2178-0.5274-0.28310.3225-0.44390.3557-0.0232-0.05010.3441-0.03560.2624206.542496.674454.4872
171.6399-0.44220.13011.1378-0.03791.0543-0.0199-0.087-0.0243-0.01780.0019-0.11690.1440.11040.00120.36320.03210.04270.4307-0.03420.2622208.443278.946446.8272
180.8328-0.1774-0.60242.7370.33561.5042-0.06910.21660.0015-0.22340.01810.1610.0767-0.35170.09780.3671-0.0168-0.03230.5599-0.05350.3273179.914386.487738.0656
190.8902-0.1775-0.29120.6551-0.23960.39740.03590.0866-0.0792-0.05910.034-0.0448-0.0509-0.1457-0.06630.36120.0204-0.00140.4156-0.04310.2398192.397680.981945.7202
206.5459-1.2051-1.00213.3768-0.66312.0526-0.4606-0.2584-0.44690.26720.14790.13720.00150.0990.27110.42190.00580.05480.3669-0.02130.283181.888273.862867.6779
213.16720.2683-1.44870.1448-0.05190.70080.0897-0.2478-0.00750.14030.0506-0.0650.03210.2209-0.10140.41880.0066-0.02660.5371-0.03560.2965198.042681.910864.8628
221.1763-0.0028-0.06833.95320.06880.7003-0.11970.04450.0667-0.23390.2544-0.1716-0.0826-0.0367-0.16750.43880.0067-0.11390.35330.0150.2987151.662566.8599100.4961
234.24762.24041.89912.55331.39062.0780.3382-0.1882-1.09090.00850.0551-0.51530.6083-0.3497-0.43820.5943-0.0649-0.17560.35750.11340.51133.45534.713994.6025
242.28570.5026-0.17661.9413-0.13021.77710.3479-0.2659-0.48850.0427-0.10590.30680.2861-0.7357-0.18340.4878-0.158-0.17070.64120.05170.4942109.951538.298186.7488
252.88290.7599-0.19081.28230.94470.940.1295-0.5452-0.43760.2993-0.19080.1050.0572-0.54980.03040.6384-0.124-0.14320.76610.08530.4132118.257243.964499.3703
262.91331.45180.58372.36390.380.12170.06130.163-0.4188-0.0322-0.0223-0.06540.16-0.3381-0.20340.50590.0559-0.17490.3688-0.01320.3466127.532541.758279.5611
272.36040.03690.72371.40740.65821.6579-0.080.0310.0878-0.2423-0.00370.0434-0.1981-0.1570.07770.57520.0296-0.07660.2851-0.0230.2724136.199561.412580.658
281.15190.38150.71831.11460.32790.978-0.0027-0.2273-0.01920.0293-0.02540.1066-0.1182-0.30480.04960.46720.0505-0.0690.45330.00790.2668127.590357.466992.1588
291.6929-0.50830.36052.0099-1.05761.4144-0.0563-0.23940.0820.00540.2077-0.1383-0.1502-0.3604-0.20250.46140.0469-0.08730.4193-0.03820.2574141.678866.4146105.8663
303.7567-2.31263.07591.5751-1.8342.68180.3481-0.7276-1.013-0.04970.13950.16750.5035-0.2263-0.5050.5309-0.0561-0.08530.34840.01640.5088163.821840.8746120.1164
311.79450.4087-0.24761.20471.33952.28340.1695-0.168-0.32320.11980.0846-0.19970.08210.0971-0.19380.41220.0315-0.10110.33160.0560.3466183.850951.155129.5264
325.295-0.19611.53263.671-0.08683.1332-0.01250.0891-0.1066-0.2940.0282-0.4832-0.01390.0442-0.02110.33960.0223-0.02580.312-0.05650.2999190.820750.3453118.0811
331.2466-0.50170.43730.766-0.19370.61970.0718-0.1248-0.1281-0.0913-0.02280.09080.02-0.0962-0.02870.44930.0079-0.06330.37210.02920.2786161.603161.0273126.3698
341.49790.1834-0.55681.55680.36680.52640.1609-0.2877-0.0552-0.0391-0.0870.1398-0.0362-0.0874-0.03570.4680.009-0.08530.4480.00330.2523164.048270135.973
351.4898-0.2910.66040.65380.01591.0936-0.00030.0596-0.0645-0.1617-0.0425-0.0866-0.0530.10240.04560.4772-0.0223-0.02360.31990.00380.2545167.608361.7736108.4059
361.14290.6298-0.58771.0521-0.28070.42630.175-0.04490.1032-0.0127-0.12940.0026-0.08980.0671-0.0560.47130.08750.00290.42820.00350.2278116.486992.765131.5586
370.72740.2527-0.14280.4518-0.1420.46170.06990.01810.0125-0.0256-0.05170.0004-0.0886-0.01590.00410.41230.05620.01020.4043-0.02430.2617108.159679.626139.8567
380.60020.5942-0.16831.5973-1.05251.24330.0386-0.03250.09690.10.01460.0838-0.2050.0612-0.04550.3940.0750.05040.3728-0.03220.2449129.8955117.559510.0934
390.7152-0.02650.20971.8412-1.09171.2001-0.0827-0.1176-0.04540.11670.08110.07210.13010.1007-0.00420.39330.05080.04170.3892-0.01010.2282134.5347108.44232.6999
400.68890.1097-0.10290.7465-0.50450.94520.01-0.1280.07190.1327-0.0658-0.0936-0.07340.16210.09070.47680.04740.0270.46340.00350.2925137.5333112.621712.9695
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 108 through 139 )
2X-RAY DIFFRACTION2chain 'A' and (resid 140 through 172 )
3X-RAY DIFFRACTION3chain 'A' and (resid 173 through 237 )
4X-RAY DIFFRACTION4chain 'A' and (resid 238 through 272 )
5X-RAY DIFFRACTION5chain 'A' and (resid 273 through 322 )
6X-RAY DIFFRACTION6chain 'A' and (resid 323 through 378 )
7X-RAY DIFFRACTION7chain 'A' and (resid 379 through 459 )
8X-RAY DIFFRACTION8chain 'A' and (resid 460 through 574 )
9X-RAY DIFFRACTION9chain 'B' and (resid 108 through 172 )
10X-RAY DIFFRACTION10chain 'B' and (resid 173 through 339 )
11X-RAY DIFFRACTION11chain 'B' and (resid 340 through 574 )
12X-RAY DIFFRACTION12chain 'C' and (resid 108 through 199 )
13X-RAY DIFFRACTION13chain 'C' and (resid 200 through 353 )
14X-RAY DIFFRACTION14chain 'C' and (resid 354 through 574 )
15X-RAY DIFFRACTION15chain 'D' and (resid 108 through 172 )
16X-RAY DIFFRACTION16chain 'D' and (resid 173 through 199 )
17X-RAY DIFFRACTION17chain 'D' and (resid 200 through 369 )
18X-RAY DIFFRACTION18chain 'D' and (resid 370 through 429 )
19X-RAY DIFFRACTION19chain 'D' and (resid 430 through 514 )
20X-RAY DIFFRACTION20chain 'D' and (resid 515 through 548 )
21X-RAY DIFFRACTION21chain 'D' and (resid 549 through 574 )
22X-RAY DIFFRACTION22chain 'E' and (resid 109 through 172 )
23X-RAY DIFFRACTION23chain 'E' and (resid 173 through 199 )
24X-RAY DIFFRACTION24chain 'E' and (resid 200 through 287 )
25X-RAY DIFFRACTION25chain 'E' and (resid 288 through 322 )
26X-RAY DIFFRACTION26chain 'E' and (resid 323 through 353 )
27X-RAY DIFFRACTION27chain 'E' and (resid 354 through 412 )
28X-RAY DIFFRACTION28chain 'E' and (resid 413 through 574 )
29X-RAY DIFFRACTION29chain 'F' and (resid 108 through 172 )
30X-RAY DIFFRACTION30chain 'F' and (resid 173 through 198 )
31X-RAY DIFFRACTION31chain 'F' and (resid 199 through 267 )
32X-RAY DIFFRACTION32chain 'F' and (resid 268 through 309 )
33X-RAY DIFFRACTION33chain 'F' and (resid 310 through 410 )
34X-RAY DIFFRACTION34chain 'F' and (resid 411 through 459 )
35X-RAY DIFFRACTION35chain 'F' and (resid 460 through 574 )
36X-RAY DIFFRACTION36chain 'G' and (resid 109 through 219 )
37X-RAY DIFFRACTION37chain 'G' and (resid 220 through 574 )
38X-RAY DIFFRACTION38chain 'H' and (resid 109 through 309 )
39X-RAY DIFFRACTION39chain 'H' and (resid 310 through 412 )
40X-RAY DIFFRACTION40chain 'H' and (resid 413 through 574 )

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