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Open data
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Basic information
Entry | Database: PDB / ID: 6wu7 | ||||||
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Title | Human Calcium and Integrin Binding Protein 3 E150Q K151H | ||||||
![]() | Calcium and integrin-binding family member 3 | ||||||
![]() | METAL BINDING PROTEIN / EF-hand / Mechanotransduction / hearing | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shapiro, L. / Dionne, G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: CIB2 and CIB3 are auxiliary subunits of the mechanotransduction channel of hair cells. Authors: Liang, X. / Qiu, X. / Dionne, G. / Cunningham, C.L. / Pucak, M.L. / Peng, G. / Kim, Y.H. / Lauer, A. / Shapiro, L. / Muller, U. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 242 KB | Display | ![]() |
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PDB format | ![]() | 188.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 5.6 MB | Display | ![]() |
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Full document | ![]() | 5.7 MB | Display | |
Data in XML | ![]() | 19.2 KB | Display | |
Data in CIF | ![]() | 28.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6wu5SC ![]() 6wudC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 22317.143 Da / Num. of mol.: 2 / Mutation: E150Q, K151H Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-ACT / | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.55 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 10% PEG 8000 0.2M Calcium Acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Aug 7, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.46 Å / Relative weight: 1 |
Reflection | Resolution: 1.837→73.08 Å / Num. obs: 44453 / % possible obs: 96.93 % / Redundancy: 18 % / Biso Wilson estimate: 28.59 Å2 / CC1/2: 0.998 / Net I/σ(I): 42.15 |
Reflection shell | Resolution: 1.837→1.902 Å / Mean I/σ(I) obs: 7.12 / Num. unique obs: 3797 / CC1/2: 0.983 / % possible all: 84.12 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6WU5 Resolution: 1.84→73.08 Å / SU ML: 0.1693 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.1254 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.59 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.84→73.08 Å
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Refine LS restraints |
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LS refinement shell |
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