National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
HHSN272201700060C
United States
Citation
Journal: To Be Published Title: Crystal structure of gamma-aminobutyrate aminotransferase PuuE from Klebsiella pneumoniae in complex with PLP Authors: Stogios, P.J.
Mass: 18.015 Da / Num. of mol.: 178 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
N
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.16 Å3/Da / Density % sol: 43.1 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.2 M sodium chloride, 0.1 M Bis-Tris pH 5.5, 25% PEG3350, 5 mM PLP, GABA. Cryoprotectant 7% gycerol 7% Ethylene glycol 7% Sucrose followed by Paratone followed by annealing in 25% Ethylene Glycol
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N