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Open data
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Basic information
Entry | Database: PDB / ID: 6wem | ||||||
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Title | Crimson 0.9 | ||||||
![]() | mCrimson 0.9 | ||||||
![]() | FLUORESCENT PROTEIN / engineered protein | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ataie, N. / Tran Tang, C. / Sens, A. / Lin, M.Z. / Chu, J. / Ng, H.L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crimson 0.9 Authors: Ataie, N. / Tran Tang, C. / Sens, A. / Lin, M.Z. / Chu, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 334 KB | Display | ![]() |
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PDB format | ![]() | 272.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 454.3 KB | Display | ![]() |
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Full document | ![]() | 467.5 KB | Display | |
Data in XML | ![]() | 36.6 KB | Display | |
Data in CIF | ![]() | 49.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3ip2S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25567.150 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.48 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.2 M MgCl2, 0.1 M Bis-Tris HCl pH 6.5, and 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 77 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 31, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.04→40 Å / Num. obs: 53213 / % possible obs: 99.1 % / Redundancy: 7.05 % / Rsym value: 0.108 / Net I/σ(I): 12.12 |
Reflection shell | Resolution: 2.04→2.16 Å / Mean I/σ(I) obs: 2.2 / Num. unique obs: 8162 / Rsym value: 0.874 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3IP2 Resolution: 2.04→40 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.933 / SU B: 11.626 / SU ML: 0.146 / Cross valid method: FREE R-VALUE / ESU R: 0.24 / ESU R Free: 0.188 Details: HYDROGENS HAVE BEEN ADDED IN THEIR RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.78 Å2
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Refinement step | Cycle: LAST / Resolution: 2.04→40 Å
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Refine LS restraints |
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