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- PDB-6vvj: Cap1G-TPUA -

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Basic information

Entry
Database: PDB / ID: 6vvj
TitleCap1G-TPUA
ComponentsRNA (130-MER)
KeywordsRNA / capped RNA / viral / MAL strain / MAL / 5' leader / Leader RNA / covered cap
Function / homologyRNA / RNA (> 10) / RNA (> 100)
Function and homology information
Biological speciesHuman immunodeficiency virus 1
MethodSOLUTION NMR / simulated annealing
AuthorsSummers, M.F. / Brown, J.D.
Funding support United States, 5items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)8R01 AI50498 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)U54 AI150470 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)F31 GM123803 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)MARC U*STAR 2T34 GM008663 United States
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Science / Year: 2020
Title: Structural basis for transcriptional start site control of HIV-1 RNA fate.
Authors: Brown, J.D. / Kharytonchyk, S. / Chaudry, I. / Iyer, A.S. / Carter, H. / Becker, G. / Desai, Y. / Glang, L. / Choi, S.H. / Singh, K. / Lopresti, M.W. / Orellana, M. / Rodriguez, T. / Oboh, U. ...Authors: Brown, J.D. / Kharytonchyk, S. / Chaudry, I. / Iyer, A.S. / Carter, H. / Becker, G. / Desai, Y. / Glang, L. / Choi, S.H. / Singh, K. / Lopresti, M.W. / Orellana, M. / Rodriguez, T. / Oboh, U. / Hijji, J. / Ghinger, F.G. / Stewart, K. / Francis, D. / Edwards, B. / Chen, P. / Case, D.A. / Telesnitsky, A. / Summers, M.F.
History
DepositionFeb 18, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 29, 2020Provider: repository / Type: Initial release
Revision 1.1May 20, 2020Group: Database references / Structure summary / Category: citation / citation_author / struct
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _struct.title
Revision 1.2Jun 14, 2023Group: Database references / Other / Category: database_2 / pdbx_database_status
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_nmr_data
Revision 2.0Mar 6, 2024Group: Data collection / Non-polymer description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / entity
Item: _chem_comp.formula / _chem_comp.formula_weight / _entity.formula_weight
Revision 2.1May 15, 2024Group: Database references / Category: database_2 / Item: _database_2.pdbx_DOI

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: RNA (130-MER)


Theoretical massNumber of molelcules
Total (without water)41,9231
Polymers41,9231
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area540 Å2
ΔGint1 kcal/mol
Surface area23750 Å2
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 20target function
RepresentativeModel #1lowest energy

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Components

#1: RNA chain RNA (130-MER)


Mass: 41922.785 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Human immunodeficiency virus 1
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDSample stateSpectrometer-IDType
111isotropic12D 1H-1H NOESY
122isotropic12D 1H-1H NOESY
133isotropic12D 1H-1H NOESY
144isotropic12D 1H-1H NOESY
155isotropic12D 1H-1H NOESY
166isotropic12D 1H-1H NOESY
177isotropic12D 1H-1H NOESY
188isotropic12D 1H-1H NOESY
299isotropic12D 1H-1H NOESY
21010isotropic12D 1H-1H NOESY
21111isotropic12D 1H-1H NOESY
11212isotropic12D 1H-1H NOESY
11313isotropic12D 1H-1H NOESY
11414isotropic12D 1H-1H NOESY
11515isotropic12D 1H-1H NOESY
11616isotropic12D 1H-1H NOESY
11717isotropic12D 1H-1H NOESY
11818isotropic12D 1H-1H NOESY
11919isotropic12D 1H-1H NOESY
12020isotropic12D 1H-1H NOESY
12121isotropic12D 1H-1H NOESY
12222isotropic12D 1H-1H NOESY
12323isotropic12D 1H-1H NOESY
12424isotropic12D 1H-1H NOESY

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Sample preparation

Details
TypeSolution-IDContentsLabelSolvent system
solution1150 uM 1H Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_1H100% D2O
solution2150 uM Gh Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_Gh100% D2O
solution3150 uM A2rGr Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_A2rGr100% D2O
solution4150 uM GhU6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_GhU6r100% D2O
solution5150 uM U6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_U6r100% D2O
solution6150 uM C6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_C6r100% D2O
solution7150 uM C6rU6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_C6rU6r100% D2O
solution8150 uM AhCh Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2OCap1G-TPUA_AhCh100% D2O
solution9150 uM U6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 90% H2O/10% D2OCap1G-TPUA_U6r-H2O90% H2O/10% D2O
solution10150 uM 1H Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 90% H2O/10% D2OCap1G-TPUA_1H-H2O90% H2O/10% D2O
solution11150 uM Gh Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 90% H2O/10% D2OCap1G-TPUA_Gh-H2O90% H2O/10% D2O
solution12150 uM 1H 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_1H100% D2O
solution13150 uM Ah 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_Ah100% D2O
solution14150 uM Ch 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_Ch100% D2O
solution15150 uM Uh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_Uh100% D2O
solution16150 uM A2rCr 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_A2rCr100% D2O
solution17150 uM A2rUr 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_A2rUr100% D2O
solution18150 uM C6rUr 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_C6rUr100% D2O
solution19150 uM CrU6r 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_CrU6r100% D2O
solution20150 uM AhCh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_AhCh100% D2O
solution21150 uM ChGh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_ChGh100% D2O
solution22150 uM GhUh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_GhUh100% D2O
solution23150 uM AhUh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O2G-TPUA_AhUh100% D2O
solution24150 uM AhGh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 M KCl, 100% D2O2G-TPUA_AhGh100% D2O
Sample
Conc. (mg/ml)ComponentIsotopic labelingSolution-ID
150 uMCap1G-TPUA1H1
10 mMKH2PO4Deuterated1
1 mMMgCl2natural abundance1
122 mMKClnatural abundance1
150 uMCap1G-TPUAGh2
10 mMKH2PO4Deuterated2
1 mMMgCl2natural abundance2
122 mMKClnatural abundance2
150 uMCap1G-TPUAA2rGr3
10 mMKH2PO4Deuterated3
1 mMMgCl2natural abundance3
122 mMKClnatural abundance3
150 uMCap1G-TPUAGhU6r4
10 mMKH2PO4Deuterated4
1 mMMgCl2natural abundance4
122 mMKClnatural abundance4
150 uMCap1G-TPUAU6r5
10 mMKH2PO4Deuterated5
1 mMMgCl2natural abundance5
122 mMKClnatural abundance5
150 uMCap1G-TPUAC6r6
10 mMKH2PO4Deuterated6
1 mMMgCl2natural abundance6
122 mMKClnatural abundance6
150 uMCap1G-TPUAC6rU6r7
10 mMKH2PO4Deuterated7
1 mMMgCl2natural abundance7
122 mMKClnatural abundance7
150 uMCap1G-TPUAAhCh8
10 mMKH2PO4Deuterated8
1 mMMgCl2natural abundance8
122 mMKClnatural abundance8
150 uMCap1G-TPUAU6r9
10 mMKH2PO4Deuterated9
1 mMMgCl2natural abundance9
122 mMKClnatural abundance9
150 uMCap1G-TPUA1H10
10 mMKH2PO4Deuterated10
1 mMMgCl2natural abundance10
122 mMKClnatural abundance10
150 uMCap1G-TPUAGh11
10 mMKH2PO4Deuterated11
1 mMMgCl2natural abundance11
122 mMKClnatural abundance11
150 uM2G-TPUA1H12
10 mMKH2PO4Deuterated12
1 mMMgCl2natural abundance12
122 mMKClnatural abundance12
150 uM2G-TPUAAh13
10 mMKH2PO4Deuterated13
1 mMMgCl2natural abundance13
122 mMKClnatural abundance13
150 uM2G-TPUACh14
10 mMKH2PO4Deuterated14
1 mMMgCl2natural abundance14
122 mMKClnatural abundance14
150 uM2G-TPUAUh15
10 mMKH2PO4Deuterated15
1 mMMgCl2natural abundance15
122 mMKClnatural abundance15
150 uM2G-TPUAA2rCr16
10 mMKH2PO4Deuterated16
1 mMMgCl2natural abundance16
122 mMKClnatural abundance16
150 uM2G-TPUAA2rUr17
10 mMKH2PO4Deuterated17
1 mMMgCl2natural abundance17
122 mMKClnatural abundance17
150 uM2G-TPUAC6rUr18
10 mMKH2PO4Deuterated18
1 mMMgCl2natural abundance18
122 mMKClnatural abundance18
150 uM2G-TPUACrU6r19
10 mMKH2PO4Deuterated19
1 mMMgCl2natural abundance19
122 mMKClnatural abundance19
150 uM2G-TPUAAhCh20
10 mMKH2PO4Deuterated20
1 mMMgCl2natural abundance20
122 mMKClnatural abundance20
150 uM2G-TPUAChGh21
10 mMKH2PO4Deuterated21
1 mMMgCl2natural abundance21
122 mMKClnatural abundance21
150 uM2G-TPUAGhUh22
10 mMKH2PO4Deuterated22
1 mMMgCl2natural abundance22
122 mMKClnatural abundance22
150 uM2G-TPUAAhUh23
10 mMKH2PO4Deuterated23
1 mMMgCl2natural abundance23
122 mMKClnatural abundance23
150 uM2G-TPUAAhGh24
10 mMKH2PO4Deuterated24
1 mMMgCl2natural abundance24
122 MKClnatural abundance24
Sample conditionsIonic strength: 10 mM KH2PO4, 1 mM MgCl2, 122 mM KCl mM / Label: PI-D2O / pH: 7.4 Not defined / Pressure: 1 atm / Temperature: 308 K

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NMR measurement

NMR spectrometerType: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz

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Processing

NMR software
NameDeveloperClassification
NMRFxJohnson, One Moon Scientificprocessing
NMRViewJohnson, One Moon Scientificdata analysis
NMRViewJohnson, One Moon Scientificpeak picking
NMRViewJohnson, One Moon Scientificchemical shift assignment
CYANAGuntert, Mumenthaler and Wuthrichgeometry optimization
CYANAGuntert, Mumenthaler and Wuthrichstructure calculation
AmberCase, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollmanstructure calculation
AmberCase, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollmanrefinement
CYANAGuntert, Mumenthaler and Wuthrichrefinement
RefinementMethod: simulated annealing / Software ordinal: 9 / Details: distance geometry, molecular dynamics
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 20 / Conformers submitted total number: 20

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