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- PDB-6vm8: SILv44 T cell receptor bound to HLA-A2 presenting gp100T2M peptid... -

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Open data


ID or keywords:

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Basic information

Entry
Database: PDB / ID: 6vm8
TitleSILv44 T cell receptor bound to HLA-A2 presenting gp100T2M peptide (IMDQVPFSV)
Components
  • (SILv44 T cell receptor ...) x 2
  • Beta-2-microglobulin
  • MHC class I antigen, A-2 alpha chain
  • Melanocyte protein PMEL
KeywordsIMMUNE SYSTEM / TCR / MHC class I / HLA A2 / autoimmune / vitiligo / melanoma
Function / homology
Function and homology information


positive regulation of melanin biosynthetic process / melanin biosynthetic process / melanosome membrane / multivesicular body, internal vesicle / cis-Golgi network membrane / multivesicular body membrane / melanosome organization / Regulation of MITF-M-dependent genes involved in pigmentation / antigen processing and presentation of peptide antigen via MHC class I / early endosome lumen ...positive regulation of melanin biosynthetic process / melanin biosynthetic process / melanosome membrane / multivesicular body, internal vesicle / cis-Golgi network membrane / multivesicular body membrane / melanosome organization / Regulation of MITF-M-dependent genes involved in pigmentation / antigen processing and presentation of peptide antigen via MHC class I / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / Endosomal/Vacuolar pathway / T cell mediated cytotoxicity / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / lumenal side of endoplasmic reticulum membrane / regulation of iron ion transport / cellular response to iron(III) ion / negative regulation of iron ion transport / negative regulation of forebrain neuron differentiation / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of erythrocyte differentiation / response to molecule of bacterial origin / HFE-transferrin receptor complex / transferrin transport / MHC class I peptide loading complex / cellular response to iron ion / negative regulation of receptor-mediated endocytosis / positive regulation of T cell cytokine production / antigen processing and presentation of endogenous peptide antigen via MHC class I / MHC class I protein complex / peptide antigen assembly with MHC class II protein complex / negative regulation of neurogenesis / cellular response to nicotine / positive regulation of receptor-mediated endocytosis / MHC class II protein complex / multicellular organismal-level iron ion homeostasis / positive regulation of T cell mediated cytotoxicity / specific granule lumen / positive regulation of immune response / antigen processing and presentation of exogenous peptide antigen via MHC class II / peptide antigen binding / phagocytic vesicle membrane / recycling endosome membrane / positive regulation of T cell activation / negative regulation of epithelial cell proliferation / Interferon gamma signaling / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Modulation by Mtb of host immune system / sensory perception of smell / positive regulation of cellular senescence / tertiary granule lumen / melanosome / DAP12 signaling / MHC class II protein complex binding / T cell differentiation in thymus / late endosome membrane / negative regulation of neuron projection development / ER-Phagosome pathway / protein refolding / early endosome membrane / amyloid fibril formation / protein homotetramerization / intracellular iron ion homeostasis / learning or memory / immune response / endoplasmic reticulum lumen / Amyloid fiber formation / Golgi membrane / external side of plasma membrane / lysosomal membrane / focal adhesion / Neutrophil degranulation / endoplasmic reticulum membrane / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / cell surface / endoplasmic reticulum / Golgi apparatus / protein homodimerization activity / : / extracellular exosome / extracellular region / membrane / identical protein binding / plasma membrane / cytosol
Similarity search - Function
PKD- and KLD-Associated Transmembrane / PKAT, KLD domain / : / PKAT, KLD domain / PMEL/NMB N-terminal domain / PKD domain / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain ...PKD- and KLD-Associated Transmembrane / PKAT, KLD domain / : / PKAT, KLD domain / PMEL/NMB N-terminal domain / PKD domain / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
CITRATE ANION / Major histocompatibility complex, class I, A / MHC class I antigen / Melanocyte protein PMEL / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.41 Å
AuthorsSmith, A.R. / Baker, B.M.
Funding support1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2021
Title: Structurally silent peptide anchor modifications allosterically modulate T cell recognition in a receptor-dependent manner.
Authors: Smith, A.R. / Alonso, J.A. / Ayres, C.M. / Singh, N.K. / Hellman, L.M. / Baker, B.M.
History
DepositionJan 27, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 25, 2020Provider: repository / Type: Initial release
Revision 1.1Jun 9, 2021Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 30, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: MHC class I antigen, A-2 alpha chain
E: SILv44 T cell receptor beta chain
D: SILv44 T cell receptor alpha chain
B: Beta-2-microglobulin
C: Melanocyte protein PMEL
hetero molecules


Theoretical massNumber of molelcules
Total (without water)95,9386
Polymers95,7495
Non-polymers1891
Water1,60389
1
A: MHC class I antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: Melanocyte protein PMEL

E: SILv44 T cell receptor beta chain
D: SILv44 T cell receptor alpha chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)95,9386
Polymers95,7495
Non-polymers1891
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation4_545x+1/2,-y-1/2,-z1
Buried area11290 Å2
ΔGint-57 kcal/mol
Surface area37630 Å2
MethodPISA
Unit cell
Length a, b, c (Å)68.269, 94.445, 143.824
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 2 types, 2 molecules AB

#1: Protein MHC class I antigen, A-2 alpha chain


Mass: 31854.203 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-A / Plasmid: pHN1 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-Blue / References: UniProt: A0A5B8RNS7, UniProt: A0A140T913*PLUS
#4: Protein Beta-2-microglobulin


Mass: 11879.356 Da / Num. of mol.: 1 / Mutation: 0
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Plasmid: pHN1 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-Blue / References: UniProt: P61769

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SILv44 T cell receptor ... , 2 types, 2 molecules ED

#2: Protein SILv44 T cell receptor beta chain


Mass: 27692.809 Da / Num. of mol.: 1 / Mutation: 0
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRBV19, TRBC2 / Plasmid: pGMT7 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS
#3: Protein SILv44 T cell receptor alpha chain


Mass: 23287.006 Da / Num. of mol.: 1 / Mutation: 0
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRAV8-1, TRAC / Plasmid: pGMT7 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS

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Protein/peptide , 1 types, 1 molecules C

#5: Protein/peptide Melanocyte protein PMEL / ME20-M / ME20M / Melanocyte protein Pmel 17 / Melanocytes lineage-specific antigen GP100 / Melanoma- ...ME20-M / ME20M / Melanocyte protein Pmel 17 / Melanocytes lineage-specific antigen GP100 / Melanoma-associated ME20 antigen / P1 / P100 / Premelanosome protein / Silver locus protein homolog


Mass: 1035.213 Da / Num. of mol.: 1 / Fragment: epitope (UNP residues 209-217) / Mutation: T210M / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P40967

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Non-polymers , 2 types, 90 molecules

#6: Chemical ChemComp-FLC / CITRATE ANION


Mass: 189.100 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H5O7
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 89 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.42 Å3/Da / Density % sol: 49.2 %
Crystal growTemperature: 296 K / Method: vapor diffusion, hanging drop / Details: 20% v/v PEG3350, 236 mM ammonium citrate dibasic

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 12, 2017
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.4→50 Å / Num. obs: 36792 / % possible obs: 99.7 % / Redundancy: 8.2 % / Biso Wilson estimate: 36.2 Å2 / Rmerge(I) obs: 0.149 / Rpim(I) all: 0.053 / Rrim(I) all: 0.159 / Χ2: 1.756 / Net I/σ(I): 23.2
Reflection shellResolution: 2.4→2.44 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.76 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 1759 / CC1/2: 0.568 / CC star: 0.851 / Rpim(I) all: 0.379 / Rrim(I) all: 0.854 / Χ2: 1.135 / % possible all: 95.8

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Processing

Software
NameVersionClassification
PHENIX1.13_2998refinement
PHENIX1.13_2998refinement
HKL-2000data reduction
HKL-2000data scaling
MoRDaphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.41→34.17 Å / SU ML: 0.2644 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 22.9221
RfactorNum. reflection% reflection
Rfree0.2214 1904 5.45 %
Rwork0.1865 --
obs0.1885 34958 94.77 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 45.19 Å2
Refinement stepCycle: LAST / Resolution: 2.41→34.17 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6645 0 13 89 6747
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00186845
X-RAY DIFFRACTIONf_angle_d0.48089295
X-RAY DIFFRACTIONf_chiral_restr0.0413965
X-RAY DIFFRACTIONf_plane_restr0.00271214
X-RAY DIFFRACTIONf_dihedral_angle_d17.01072505
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.41-2.470.33361110.26561973X-RAY DIFFRACTION80.71
2.47-2.530.30221290.25342187X-RAY DIFFRACTION88.09
2.53-2.610.25631200.25162198X-RAY DIFFRACTION89.67
2.61-2.690.37571300.23212219X-RAY DIFFRACTION91.33
2.69-2.790.24311340.22292313X-RAY DIFFRACTION93.79
2.79-2.90.27241380.21642311X-RAY DIFFRACTION94.56
2.9-3.030.2641340.20582358X-RAY DIFFRACTION95.59
3.03-3.190.21841400.20772434X-RAY DIFFRACTION97.5
3.19-3.390.25781420.20142432X-RAY DIFFRACTION98.13
3.39-3.650.2321420.18522476X-RAY DIFFRACTION99.32
3.65-4.020.19111440.16922483X-RAY DIFFRACTION99.21
4.02-4.60.1591430.14532489X-RAY DIFFRACTION99.73
4.6-5.790.1881470.15212544X-RAY DIFFRACTION99.52
5.79-34.170.20081500.1762637X-RAY DIFFRACTION98.79
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.767415746770.108026043248-0.2093824446772.10944371366-0.3016322214992.970718011980.01612245131650.04122894235710.03367931981320.00511492315280.03663190896130.00859778372517-0.1104508069610.000347198279192-0.0551844428520.133139436120.03147399596250.006287940112190.220263371972-0.04654845267280.312003924768-23.025810283-13.8565444601-13.1165975682
25.06695860425-3.58124841668-0.9744061703424.91021141191.396718348911.935475675090.2216829366020.199415636288-0.181335219271-0.225770571278-0.2205225850780.141456995045-0.437546142871-0.37190836704-0.0304551504330.3808375675350.0766420059518-0.08317890557160.291998175995-0.05461204929090.349185679038-39.525570892815.037788899-12.9836014589
35.49098949365-2.01309820624-2.137511534824.903846018632.150959491685.52634289065-0.196672613928-0.4199523614650.2137488479580.7146947485230.07160882507880.157249461819-0.0263399729419-0.1461753232110.1064578430990.3726526034430.0398439893142-0.04437469114050.241167403528-0.02879117530740.328349401487-40.711747107916.0134744978-4.64816448787
43.214773928870.7573844742650.4638720045315.42800516791.165796219352.65989315532-0.280340904398-0.4539780565311.05561522722-0.219794594533-0.09804440792890.506397476806-0.9965278656230.2082115050230.2050186796140.492995661476-0.028016338719-0.1181731101130.337752871077-0.09396799518730.586918840718-42.96600731442.0876249989720.3289170665
52.182333474090.932136168899-0.0593019293224.877644143422.145372977153.76370998419-0.108699870465-0.2326116007780.248426658018-0.0566403527584-0.07255286773120.0909953038848-0.39264482387-0.1783866563850.1643425620270.1765072754710.0099078576849-0.03788289661870.294830745039-0.04588077461090.34102652028-45.6223898786-10.389238584822.041190917
61.362500849821.835862988310.3509922159725.41185869779-0.4723903731520.3946079305690.359163021748-0.03114039013030.1189925755810.711642145731-0.77388167281-0.0674061599258-0.0148625592817-0.142976927120.461240589570.55598471328-0.0637013974882-0.09131965404610.397185012339-0.07836850186330.541986087721-35.617746785412.699229723926.1489222938
73.50294054108-1.0109706657-2.005723036162.383924945771.560581405843.57959431527-0.0226318371359-0.0307430378142-0.10124582681-0.4411337121090.0419773317273-0.00385080082027-0.1178567068520.0756737946059-0.01969380744280.335980903704-0.04341920505620.01362051158660.184898494461-0.05255605105030.2521962753-16.04855409047.9399297939314.4773684103
88.52329160736-2.86640341593-4.451515086173.717589603542.233345781686.259895243290.2712689308560.5816752949850.510266539329-0.433821734584-0.1845944348980.238690492817-0.625331047451-0.900681632723-0.04316558165290.5027029690450.0214194624736-0.1336810677950.333239554604-0.01207847763940.346592823902-31.070364250517.268230635214.0438775249
97.928819479924.69712297793-2.816315698483.75965168604-1.268257877891.94284629130.367016704809-1.026894907680.007286130771030.231593583331-0.454249333445-0.163274800245-0.2810947930260.7382250015880.07320773495250.242327762225-0.0266765306053-0.09480536850610.453327961211-0.04561872551270.333611363433-22.0051412305-20.296331027129.3778752439
107.907279605041.76501142605-0.5124341419233.64107503033-0.7721485928482.80288861354-0.2448598063870.07071282006670.109728052289-0.1949148017240.142355004972-0.1473076434960.06386026947090.04960813142570.08385529110020.163228449889-0.01820935075150.0194898984840.267459465669-0.03205586298650.210641802291-30.1961280046-23.917162462421.9341999942
110.5760943570061.28631926948-0.8004425323072.8673350538-1.788366927371.2479090870.247534207645-0.7470110871530.162643782665-0.05958811444-0.241468327901-0.519633300950.05970246711790.401170648017-0.1383145163680.279543808704-0.06332116651220.004193113759760.423063581639-0.07917078242830.720953084191-8.21614810163-9.8121206996822.9284210865
124.50139340624-1.56610918482-0.3218499333783.027298317550.2576792832225.35275049742-0.146469960625-0.01103772360280.115009860283-0.1658467460280.0303252101573-0.583689770366-0.2222372432930.3659316681510.08283324159940.294930274919-0.1246376191830.05866781730680.316193449277-0.102780206180.381591529617-7.099048524129.6355826412323.1171438979
136.28278309252-1.73504998984-1.156206721471.934883688411.936464304158.08270629547-0.1363550760550.443017952529-0.872395864070.0358236592118-0.0381121997324-0.2564375368761.15409800286-0.08700858826860.2597136544680.387469868443-0.07841884249760.0423621873250.335321266112-0.03889800794470.353903539053-10.7433493683-0.56581609503418.7770242937
145.22662022513-1.78975816748-0.898363610466.15217239534-0.3443143249473.216128594790.0432787391232-0.2101165599670.5307659210790.2932954672520.213123336329-1.08149805409-0.4331272168120.709432733829-0.242274535640.368408285234-0.1127568462790.02215058643710.55066031091-0.08770146831460.48589381007-4.365479508219.7630192437625.1748295077
155.64443285983-2.002025561682.608894226878.4255788846-6.697999487529.71514707935-0.281426774702-0.319731086887-0.08636477546190.4864086366380.3122121029090.157183064756-0.337704355354-0.0531694651752-0.03680856627380.2131062650010.03068703657180.04280052542140.249776552501-0.0696227882240.220380430895-44.2923333047-6.22322690428-6.83669991226
165.96290410132-1.642893679441.138068016542.340599266790.151012298523.2206863534-0.02255234210670.7006467131920.655814890057-0.0940907702534-0.384330768395-0.2264832733-0.4453551618150.3114731442890.3380237384430.2039717699090.0406976543778-0.05304138973850.2948881836960.00714290398190.421830238967-46.43050980294.56332809313-19.7838153817
177.67558883936-3.429326202372.617132540484.5334720781-2.818089380623.784090834220.0180310412364-0.0218572174618-0.458963954553-0.4339547124580.1711026469840.3646117805010.194283358084-0.3264264478-0.2054130999370.263568917626-0.0191170482148-0.003200813461940.221183466839-0.09745576074860.354757220362-44.7538602534-8.52701475076-16.4383257984
183.15899685541-4.427917632972.441665041247.02825757545-3.171016010784.19063611076-0.05034575171570.5382008433680.156800942413-0.513637022864-0.09498065035960.406074145862-0.350640291402-0.3621419006150.1920924802260.3186927205440.0175362367932-0.1139835180620.410384942988-0.017469009170.464588398249-49.71704197180.924018491563-20.7286080515
193.57554344715-1.16887616078-0.05675047934661.919191521010.6129683003720.230914153628-0.439594761087-0.16265099325-0.0436520318038-0.08727121169770.6710256223060.990061567534-0.0350546315843-0.881965240601-0.2480770416180.208232544574-0.01164941435-0.0206822833970.3968987403590.06838421239490.492884641669-52.930061521-9.74258797771-10.5453318452
206.83297764063-0.00763929128444-0.1609878025179.50431307211-1.628571513444.03258251684-0.190114295523-0.3226319751050.4727331896410.6520972069890.1964582142330.40424158656-0.391268698865-0.229641796505-0.07696335663980.2491670056860.1001500977440.0516647115360.363479391258-0.02180948003630.414426382559-53.96161960984.3567591673-12.5216983555
211.57004173096-1.694261836380.09419230926414.51128653328-2.049432314511.438447619430.0542744905111-0.07407073979570.0319554113593-0.02658972694290.2167976064890.391798768541-0.1631630278090.0360535336885-0.2677534618340.137945307052-0.0136306953093-0.02882222134180.242004141299-0.05086757240770.278257682116-17.9683774377-19.1626478254-14.2036719604
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 174 )
2X-RAY DIFFRACTION2chain 'A' and (resid 175 through 197 )
3X-RAY DIFFRACTION3chain 'A' and (resid 198 through 275 )
4X-RAY DIFFRACTION4chain 'E' and (resid 2 through 24 )
5X-RAY DIFFRACTION5chain 'E' and (resid 25 through 107 )
6X-RAY DIFFRACTION6chain 'E' and (resid 108 through 122 )
7X-RAY DIFFRACTION7chain 'E' and (resid 123 through 213 )
8X-RAY DIFFRACTION8chain 'E' and (resid 214 through 242 )
9X-RAY DIFFRACTION9chain 'D' and (resid 3 through 22 )
10X-RAY DIFFRACTION10chain 'D' and (resid 23 through 107 )
11X-RAY DIFFRACTION11chain 'D' and (resid 108 through 121 )
12X-RAY DIFFRACTION12chain 'D' and (resid 122 through 155 )
13X-RAY DIFFRACTION13chain 'D' and (resid 156 through 170 )
14X-RAY DIFFRACTION14chain 'D' and (resid 171 through 203 )
15X-RAY DIFFRACTION15chain 'B' and (resid 1 through 12 )
16X-RAY DIFFRACTION16chain 'B' and (resid 13 through 31 )
17X-RAY DIFFRACTION17chain 'B' and (resid 32 through 62 )
18X-RAY DIFFRACTION18chain 'B' and (resid 63 through 78 )
19X-RAY DIFFRACTION19chain 'B' and (resid 79 through 91 )
20X-RAY DIFFRACTION20chain 'B' and (resid 92 through 100 )
21X-RAY DIFFRACTION21chain 'C' and (resid 1 through 9 )

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