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Yorodumi- PDB-6v8u: Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a m... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6v8u | ||||||
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Title | Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a modified Kaiso binding sequence (KBS) | ||||||
Components |
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Keywords | DNA BINDING PROTEIN / DNA methylation / Zinc finger / Transcriptional regulator | ||||||
Function / homology | Function and homology information regulation of immune system process / methyl-CpG binding / regulation of cytokine production / Wnt signaling pathway / DNA-binding transcription repressor activity, RNA polymerase II-specific / sequence-specific double-stranded DNA binding / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding ...regulation of immune system process / methyl-CpG binding / regulation of cytokine production / Wnt signaling pathway / DNA-binding transcription repressor activity, RNA polymerase II-specific / sequence-specific double-stranded DNA binding / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / chromatin / nucleolus / negative regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.103 Å | ||||||
Authors | Nikolova, E.N. / Stanfield, R.L. / Dyson, H.J. / Wright, P.E. | ||||||
Funding support | United States, 1items
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Citation | Journal: Biochemistry / Year: 2020 Title: A Conformational Switch in the Zinc Finger Protein Kaiso Mediates Differential Readout of Specific and Methylated DNA Sequences. Authors: Nikolova, E.N. / Stanfield, R.L. / Dyson, H.J. / Wright, P.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6v8u.cif.gz | 116.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6v8u.ent.gz | 84.9 KB | Display | PDB format |
PDBx/mmJSON format | 6v8u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6v8u_validation.pdf.gz | 249.8 KB | Display | wwPDB validaton report |
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Full document | 6v8u_full_validation.pdf.gz | 249.8 KB | Display | |
Data in XML | 6v8u_validation.xml.gz | 1 KB | Display | |
Data in CIF | 6v8u_validation.cif.gz | 3.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v8/6v8u ftp://data.pdbj.org/pub/pdb/validation_reports/v8/6v8u | HTTPS FTP |
-Related structure data
Related structure data | 6df5C 6df8C 6df9C 6dfaC 6dfbC 6dfcC 4f6mS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 16217.771 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ZBTB33, KAISO, ZNF348 / Plasmid: PET21D / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): [DNAY] / References: UniProt: Q86T24 |
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-DNA chain , 2 types, 2 molecules DE
#2: DNA chain | Mass: 5491.566 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) |
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#3: DNA chain | Mass: 5540.604 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) |
-Non-polymers , 4 types, 112 molecules
#4: Chemical | #5: Chemical | ChemComp-CL / | #6: Chemical | ChemComp-NA / | #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.62 Å3/Da / Density % sol: 65.98 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 5 Details: 0.02 M Calcium Chloride, 0.1 M Sodium Acetate, 30 % 2-methyl-2,4-pentanediol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 18, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.103→42.92 Å / Num. obs: 23073 / % possible obs: 97.56 % / Redundancy: 11.1 % / Biso Wilson estimate: 38.8250910295 Å2 / CC1/2: 0.996 / CC star: 0.999 / Rmerge(I) obs: 0.1071 / Rrim(I) all: 0.1123 / Net I/σ(I): 17.6 |
Reflection shell | Resolution: 2.103→2.178 Å / Redundancy: 6.6 % / Rmerge(I) obs: 0.7772 / Mean I/σ(I) obs: 2.83 / Num. unique obs: 1986 / CC1/2: 0.86 / CC star: 0.961 / % possible all: 86.23 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4F6M Resolution: 2.103→42.919 Å / SU ML: 0.21 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 22.07 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 137.62 Å2 / Biso mean: 56.4997 Å2 / Biso min: 22.26 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.103→42.919 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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