+Open data
-Basic information
Entry | Database: PDB / ID: 6uru | ||||||
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Title | iAChSnFR Fluorescent Acetylcholine Sensor precursor | ||||||
Components | iAChSnFR precursor | ||||||
Keywords | FLUORESCENT PROTEIN / Biosensor / sensor / neurotransmitter / acetylcholine / circularly permuted | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Borden, P.M. / Marvin, J.S. / Looger, L.L. | ||||||
Citation | Journal: To Be Published Title: iAChSnFR Fluorescent Acetylcholine Sensor precursor Authors: Borden, P.M. / Marvin, J.S. / Looger, L.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6uru.cif.gz | 207.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6uru.ent.gz | 165.1 KB | Display | PDB format |
PDBx/mmJSON format | 6uru.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ur/6uru ftp://data.pdbj.org/pub/pdb/validation_reports/ur/6uru | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 62896.988 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.55 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: Phosphate buffer |
-Data collection
Diffraction | Mean temperature: 298 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 25, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→92.9 Å / Num. obs: 36373 / % possible obs: 99.3 % / Redundancy: 5.1 % / CC1/2: 0.989 / Rmerge(I) obs: 0.141 / Net I/σ(I): 8 |
Reflection shell | Resolution: 2.6→2.74 Å / Rmerge(I) obs: 1.228 / Num. unique obs: 5167 / CC1/2: 0.299 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5f9g, 3ppn Resolution: 2.6→92.89 Å / Cor.coef. Fo:Fc: 0.936 / Cor.coef. Fo:Fc free: 0.902 / SU B: 15.025 / SU ML: 0.306 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.976 / ESU R Free: 0.337 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 177.77 Å2 / Biso mean: 53.719 Å2 / Biso min: 26.68 Å2
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Refinement step | Cycle: final / Resolution: 2.6→92.89 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.667 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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