National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35GM118093
米国
引用
ジャーナル: Mol Cell / 年: 2020 タイトル: Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery. 著者: Yixiao Zhang / Yadong Sun / Yongsheng Shi / Thomas Walz / Liang Tong / 要旨: The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall ...The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core.
履歴
登録
2019年10月23日
登録サイト: RCSB / 処理サイト: RCSB
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2019年11月27日
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2019年11月27日
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2019年11月27日
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2019年11月27日
Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
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2019年11月27日
Data content type: EM metadata / Data content type: EM metadata / Provider: repository / タイプ: Initial release
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2019年11月27日
Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.0
2019年11月27日
Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
改定 1.0
2019年11月27日
Data content type: EM metadata / Data content type: EM metadata / Provider: repository / タイプ: Initial release
改定 1.0
2019年11月27日
Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.0
2019年11月27日
Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
改定 1.0
2019年11月27日
Data content type: EM metadata / Data content type: EM metadata / Provider: repository / タイプ: Initial release
改定 1.0
2019年11月27日
Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.0
2019年11月27日
Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
Data content type: EM metadata / Data content type: EM metadata / EM metadata / Group: Data processing / Experimental summary / Data content type: EM metadata / EM metadata / カテゴリ: em_admin / em_software / Data content type: EM metadata / EM metadata / Item: _em_admin.last_update / _em_software.name